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  1. Lab Manual: Atomic Force Microscopy (AFM)‏‎ (422,715 views)
  2. 20.309 Main Page‏‎ (327,236 views)
  3. DNA Melting Part 1: Measuring Temperature and Fluorescence‏‎ (293,755 views)
  4. Main Page‏‎ (291,867 views)
  5. Electronics primer‏‎ (248,212 views)
  6. Electronics Mini-Lab‏‎ (243,336 views)
  7. Geometrical optics and ray tracing‏‎ (206,114 views)
  8. Lab Manual: Optical Microscopy‏‎ (148,587 views)
  9. Assignment 2 Part 1: Noise in images‏‎ (145,479 views)
  10. 20.345:Course Information‏‎ (145,072 views)
  11. Optics Bootcamp‏‎ (124,643 views)
  12. 20.309:Course Information‏‎ (120,601 views)
  13. DNA Melting: Simulating DNA Melting - Intermediate Topics‏‎ (115,046 views)
  14. DNA Melting Part 2: Lock-in Amplifier and Temperature Control‏‎ (111,179 views)
  15. Error analysis‏‎ (110,135 views)
  16. Assignment 4 part 2: Measure resolution‏‎ (108,323 views)
  17. Assignment 1, Part 3: Building your transillumination microscope‏‎ (106,857 views)
  18. Optical Microscopy Part 1: Brightfield Microscopy‏‎ (104,546 views)
  19. Assignment 2: fluorescence microscopy‏‎ (97,425 views)
  20. Optical detectors, noise, and the limit of detection‏‎ (90,337 views)
  21. Assignment 1, Part 2: Optics bootcamp‏‎ (89,746 views)
  22. Impedance Analysis‏‎ (89,174 views)
  23. 20.109 Main Page‏‎ (86,578 views)
  24. Assignment 3, Part 1: visualizing actin with fluorescence contrast‏‎ (85,275 views)
  25. Bode plots‏‎ (84,768 views)
  26. Limits of Detection: Data Sessions‏‎ (83,953 views)
  27. Estimating second order system parameters from noise power spectra using nonlinear regression‏‎ (83,480 views)
  28. Lab Manual: Limits of Detection‏‎ (83,476 views)
  29. DNA Melting Report Requirements‏‎ (82,693 views)
  30. Assignment 1 Overview: Transillumination microscopy‏‎ (82,674 views)
  31. Understanding log plots‏‎ (78,615 views)
  32. DNA Melting: Model function and parameter estimation by nonlinear regression‏‎ (78,334 views)
  33. Assignment 4 part 1: Make a fake image‏‎ (77,373 views)
  34. Todo List‏‎ (76,902 views)
  35. Electronics written problems‏‎ (75,575 views)
  36. Assignment 1, Part 4: Measuring magnification and bead size‏‎ (74,614 views)
  37. Spring 2020 Assignment 9‏‎ (72,165 views)
  38. DNA Melting: Simulating DNA Melting - Basics‏‎ (71,898 views)
  39. Optical Microscopy Part 3: Resolution and Stability‏‎ (71,566 views)
  40. 20.109(F18): Fall 2018 schedule‏‎ (70,801 views)
  41. Exam 2 study guide‏‎ (68,115 views)
  42. Assignment 10, Part 1: Measuring the osmotic shock response of yeast‏‎ (66,244 views)
  43. Physical optics and resolution‏‎ (65,461 views)
  44. Assignment 8, Part 3: add flow control and test your device‏‎ (64,684 views)
  45. Lab Manual: Measuring DNA Melting Curves‏‎ (62,976 views)
  46. Optical Microscopy Part 2: Fluorescence Microscopy‏‎ (62,692 views)
  47. 20.109(S19): Spring 2019 schedule‏‎ (60,634 views)
  48. Assignment 2 Part 2: Fluorescence microscopy‏‎ (60,324 views)
  49. Protocols for cell culture‏‎ (59,172 views)
  50. Optical Microscopy Part 4: Particle Tracking‏‎ (58,447 views)
  51. Assignment 2 Part 3: Build an epi-illuminator for your microscope‏‎ (57,747 views)
  52. 20.109(S17): Spring 2017 schedule‏‎ (57,619 views)
  53. Electronics boot camp lab part 1‏‎ (54,503 views)
  54. 20.109(F16): Fall 2016 schedule‏‎ (54,477 views)
  55. Microscopy report outline‏‎ (54,169 views)
  56. 20.109(S20): Spring 2020 schedule‏‎ (53,878 views)
  57. 20.109(S18): Spring 2018 schedule‏‎ (52,895 views)
  58. Cell Reservations‏‎ (52,613 views)
  59. Assignment 1, Part 1: Pre-lab questions‏‎ (51,923 views)
  60. 20.109(F20): Fall 2020 schedule‏‎ (51,034 views)
  61. 20.109(F19):Class data‏‎ (50,408 views)
  62. Input and output impedance‏‎ (50,281 views)
  63. 20.109(S19):Class data‏‎ (50,161 views)
  64. 20.109(S18):Class data‏‎ (49,444 views)
  65. Assignment 9, Part 1: Analyze two-color yeast images‏‎ (49,341 views)
  66. Manta G032 camera measurements‏‎ (48,810 views)
  67. 20.109(S21):Spring 2021 schedule‏‎ (48,212 views)
  68. MATLAB: Estimating resolution from a PSF slide image‏‎ (48,198 views)
  69. DNA Melting Thermodynamics‏‎ (47,569 views)
  70. Complex Number Review‏‎ (46,438 views)
  71. Assignment 4 part 3: Track microspheres over time‏‎ (46,071 views)
  72. 20.109(F18):Class data‏‎ (45,796 views)
  73. 20.109(F17): Fall 2017 Schedule‏‎ (43,156 views)
  74. Assignment 8, Part 2: fabricate a microfluidic device‏‎ (42,598 views)
  75. 20.109(S21):M1D1‏‎ (41,485 views)
  76. 20.109(F16):Communication‏‎ (39,823 views)
  77. 20.109(F19): Fall 2019 schedule‏‎ (39,460 views)
  78. 20.109(F15):Homework‏‎ (38,257 views)
  79. DNA Melting Report Requirements for Part 1‏‎ (38,218 views)
  80. 20.309:Lab Report Guidelines‏‎ (36,200 views)
  81. 20.109(S11):Journal club (Day8)‏‎ (36,171 views)
  82. DNA melting: Identifying the unknown sample‏‎ (35,731 views)
  83. 20.109(S16):Homework‏‎ (35,456 views)
  84. Assignment 8, Part 0: convolution practice‏‎ (35,364 views)
  85. Spring 2012:Leanna Morinishi Lab 1‏‎ (35,194 views)
  86. Flat-field correction‏‎ (34,934 views)
  87. Converting Gaussian fit to Rayleigh resolution‏‎ (34,931 views)
  88. 20.109(F18):Homework‏‎ (34,558 views)
  89. 20.109(F16):Test comet chip loading variables (Day2)‏‎ (34,248 views)
  90. Fall 2012: Journal Presentations‏‎ (33,950 views)
  91. ThorLabs OTKB optical trapping kit‏‎ (33,868 views)
  92. 20.109(S16):In situ cloning (Day1)‏‎ (33,808 views)
  93. Assignment 8, Part 2: lock-in amplifier and temperature control‏‎ (33,141 views)
  94. Laser cutter safe operating procedure‏‎ (32,563 views)
  95. 20.109(S18):Journal club presentations (Day6 and 7)‏‎ (32,458 views)
  96. 20.109‏‎ (32,063 views)
  97. Optical Microscopy: Part 4 Report Outline‏‎ (31,407 views)
  98. 20.109(S16): Spring 2016 schedule‏‎ (31,327 views)
  99. 20.109(S21):M1D2‏‎ (30,819 views)
  100. Assignment 5 Overview‏‎ (30,524 views)
  101. 20.109(S20):Journal club presentations (Day4 and 5)‏‎ (29,723 views)
  102. 20.109(S19):Journal club presentations (Day7 and 8)‏‎ (29,386 views)
  103. Real electronics‏‎ (29,324 views)
  104. Assignment 7, Part 2: measure temperature and fluorescence‏‎ (27,814 views)
  105. 20.109(S09): Module 2 oral presentations‏‎ (27,341 views)
  106. 20.109(F16):Homework‏‎ (27,328 views)
  107. 20.109(S21):M3D3‏‎ (27,155 views)
  108. Assignment 3 Overview‏‎ (27,148 views)
  109. 20.109:People‏‎ (27,019 views)
  110. 20.109(S20):Homework‏‎ (26,672 views)
  111. Spring 11:QRT-PCR‏‎ (26,625 views)
  112. Calculating MSD and Diffusion Coefficients‏‎ (26,380 views)
  113. 20.109(S21):Homework‏‎ (26,284 views)
  114. 20.109(S08):Data analysis (Day7)‏‎ (26,110 views)
  115. 20.109(F16):Lab tour‏‎ (25,912 views)
  116. 20.109(F12)‏‎ (25,076 views)
  117. 20.109(S13):Journal club II (Day8)‏‎ (24,955 views)
  118. 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)‏‎ (24,879 views)
  119. 20.109(S17):Homework‏‎ (24,878 views)
  120. 20.109(F15): Fall 2015 schedule‏‎ (24,740 views)
  121. 20.109(F21):Homework‏‎ (24,633 views)
  122. Problem Set 1‏‎ (24,068 views)
  123. 20.109(S21):M1D7‏‎ (24,038 views)
  124. Electronics bootcamp II: feedback systems‏‎ (23,708 views)
  125. 20.109(S21):M2D6‏‎ (23,262 views)
  126. 20.109(S18):Homework‏‎ (22,939 views)
  127. Aperture and field stops‏‎ (22,870 views)
  128. 20.109(F21):Fall 2021 schedule‏‎ (22,763 views)
  129. 20.109(S14):Journal club II (Day8)‏‎ (22,702 views)
  130. Optical aberrations‏‎ (22,650 views)
  131. 20.109(F17):Journal club II (Day 6)‏‎ (22,583 views)
  132. 20.109(S16):Journal Club II (Day8)‏‎ (22,413 views)
  133. Spring 2011:Optical Trapping Lab‏‎ (22,396 views)
  134. Spring 2011:3D PSF lab‏‎ (22,353 views)
  135. 20.109(S07):Start-up genome engineering‏‎ (22,085 views)
  136. 20.109(F20):Lab tour‏‎ (22,035 views)
  137. 20.109(S22):Class data‏‎ (22,030 views)
  138. 20.109(S22):Spring 2022 schedule‏‎ (21,688 views)
  139. 20.109(F20):M3D4‏‎ (21,679 views)
  140. Assignment 10 Overview‏‎ (21,268 views)
  141. 20.109(S21):M1D4‏‎ (21,252 views)
  142. 20.109(S17):Journal Club II (Day8)‏‎ (21,084 views)
  143. Understanding the lock-in amplifier‏‎ (21,033 views)
  144. Optical microscopy lab wiki pages‏‎ (20,870 views)
  145. DNA melting lab wiki pages‏‎ (20,721 views)
  146. 20.109(F16):Journal club II (Day 6)‏‎ (20,709 views)
  147. Sandbox‏‎ (20,550 views)
  148. Spring 2020 Assignment 8‏‎ (20,510 views)
  149. Assignment 5, Part 1: MSD difference tracking and microscope stability‏‎ (20,341 views)
  150. 20.109(F20):M1D2‏‎ (20,175 views)
  151. Assignment 4: finding and measuring things‏‎ (20,019 views)
  152. 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)‏‎ (19,898 views)
  153. Spring 2012: Ajoke Jumoke Williams‏‎ (19,842 views)
  154. Spring 2012:LFM Documentation‏‎ (19,632 views)
  155. Confocal Wiki‏‎ (19,627 views)
  156. 20.109(F07):People‏‎ (19,536 views)
  157. 20.109(S21):M3D2‏‎ (19,294 views)
  158. Protocols‏‎ (19,279 views)
  159. Williams: Optical Trapping Lab 1‏‎ (19,208 views)
  160. Optical trap‏‎ (19,076 views)
  161. 20.109(F18):Journal club presentation (Day4 and 6)‏‎ (19,008 views)
  162. 20.109(F20):M2D1‏‎ (18,995 views)
  163. 20.109(F19):Journal club presentation (Day4 and 6)‏‎ (18,614 views)
  164. 20.109(S10):People‏‎ (18,463 views)
  165. Assignment 5, Part 2: live cell particle tracking of endocytosed beads‏‎ (18,453 views)
  166. 20.109(S07): Measuring calcium in vitro‏‎ (18,437 views)
  167. 20.109(F15):Journal Club II (Day8)‏‎ (18,413 views)
  168. Photobleaching Model‏‎ (18,373 views)
  169. 20.109(S21):Journal club presentation‏‎ (18,301 views)
  170. 20.109(F11)‏‎ (18,219 views)
  171. Assignment 8 Overview: flow channel & two-color microscope‏‎ (18,143 views)
  172. 20.109(S21):M3D1‏‎ (18,130 views)
  173. 20.109(F12) Pre-Proposal: Engineering Viral Magnetic Nanoparticles for Magnetic Hyperthermic Cancer Therapy‏‎ (18,129 views)
  174. 20.109(S21):M1D6‏‎ (18,072 views)
  175. 20.109(S07): Student presentations, module 2‏‎ (18,035 views)
  176. 20.109(S21):M1D3‏‎ (18,031 views)
  177. In silico cloning of pdCas9 construct‏‎ (18,028 views)
  178. Assignment 3, Part 2: experimental design with fluorescence‏‎ (18,020 views)
  179. Assignment 8 Overview‏‎ (17,755 views)
  180. Assignment 9 Overview‏‎ (17,747 views)
  181. 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)‏‎ (17,505 views)
  182. 20.109: Spring 2007 schedule‏‎ (17,504 views)
  183. Two-color Yeast Protocols‏‎ (17,445 views)
  184. 20.109(S10):Journal club (Day8)‏‎ (17,443 views)
  185. 20.345:Main page‏‎ (17,325 views)
  186. 20.109(S19):Purify protein for secondary assays(Day2)‏‎ (17,323 views)
  187. Assignment 7 Overview‏‎ (17,266 views)
  188. 20.109(F20):M1D5‏‎ (17,073 views)
  189. Assignment 6, Part 2: Electronics bootcamp‏‎ (16,844 views)
  190. 20.109(S14): Spring 2014 schedule‏‎ (16,696 views)
  191. 20.109(F12): Fall 2012 schedule‏‎ (16,570 views)
  192. 20.345:Final project proposal‏‎ (16,558 views)
  193. 20.109(S21):M3D4‏‎ (16,491 views)
  194. 20.109(S13): Spring 2013 schedule‏‎ (16,407 views)
  195. Assignment 9, Part 1: model function‏‎ (16,382 views)
  196. 20.109(F19):Homework‏‎ (16,372 views)
  197. Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking‏‎ (16,320 views)
  198. Matlab:Image Processing for Fluorescent Microscopy‏‎ (16,206 views)
  199. Assignment 8, Part 2: build a two-color microscope‏‎ (16,142 views)
  200. 20.109(F19):Treat cells for gamma-H2AX (Day2)‏‎ (16,048 views)
  201. 20.309:Safety‏‎ (16,004 views)
  202. 20.109(S07): Start-up expression engineering‏‎ (15,957 views)
  203. Temporary Optics Bootcamp‏‎ (15,951 views)
  204. 20.109(S23):Spring 2023 schedule‏‎ (15,872 views)
  205. 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)‏‎ (15,829 views)
  206. Matlab: Scalebars‏‎ (15,741 views)
  207. 20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)‏‎ (15,637 views)
  208. Spring 11:Chemotaxis Assay‏‎ (15,542 views)
  209. Assignment 5, Part 1: viscosity and diffusivity in glycerol-water mixtures‏‎ (15,432 views)
  210. 20.109(F15)‏‎ (15,393 views)
  211. 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)‏‎ (15,378 views)
  212. 20.109(F16):Module 1‏‎ (15,361 views)
  213. 20.109(F20):M2D3‏‎ (15,296 views)
  214. 20.109(S18):Analyze small microarray data and induce protein expression (Day2)‏‎ (15,234 views)
  215. 20.109(S09):People‏‎ (15,088 views)
  216. 20.109(S21):Module 1‏‎ (15,050 views)
  217. 20.109(S20):Identify clones to characterize (Day3)‏‎ (15,030 views)
  218. 20.109(F08): Mod 2 Day 1 Protein engineering with PCR‏‎ (14,970 views)
  219. 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)‏‎ (14,827 views)
  220. 20.109(F16):Prepare comet chips (Day1)‏‎ (14,794 views)
  221. 20.109(F22):Fall 2022 schedule‏‎ (14,766 views)
  222. 20.109(S19):Evaluate protein purity and concentration (Day4)‏‎ (14,754 views)
  223. 20.109(S19):Homework‏‎ (14,648 views)
  224. Assignment 7, Part 1: op amp golden rules questions‏‎ (14,608 views)
  225. 20.109(S07): Agarose gel electrophoresis‏‎ (14,538 views)
  226. 20.109(F15):Prepare expression system (Day2)‏‎ (14,481 views)
  227. 20.109(F16): Assignments‏‎ (14,291 views)
  228. 20.109(F20):M1D6‏‎ (14,171 views)
  229. Assignment 11: Tell us about your lab visit‏‎ (14,014 views)
  230. 20.109(S16):Design mutation primers (Day2)‏‎ (13,970 views)
  231. 20.109(S11):People‏‎ (13,947 views)
  232. Assignment 6 Overview‏‎ (13,885 views)
  233. 20.109(F20):Homework‏‎ (13,883 views)
  234. 20.109(S20):Purify TDP43 protein (Day2)‏‎ (13,867 views)
  235. Procedure: Diffusion in biological gels‏‎ (13,823 views)
  236. 20.109(F15):Induce protein and evaluate DNA (Day3)‏‎ (13,821 views)
  237. Spring 2012:Leanna Morinishi‏‎ (13,749 views)
  238. 20.109(F16):Confirm gRNA sequence (Day5)‏‎ (13,743 views)
  239. 20.109(S17):Complete data analysis (Day6)‏‎ (13,742 views)
  240. Assignment 9 Overview: Analyzing yeast images‏‎ (13,676 views)
  241. Using tfest to find a system model‏‎ (13,674 views)
  242. 20.109(S21):M2D7‏‎ (13,619 views)
  243. 20.109(F09): Fall 2009 schedule‏‎ (13,596 views)
  244. 20.109(S20):Analyze titration curves (Day5)‏‎ (13,595 views)
  245. 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)‏‎ (13,594 views)
  246. Spring 2020 Assignment 6‏‎ (13,590 views)
  247. 20.109(F09)‏‎ (13,554 views)
  248. 20.109(F18): Assignments‏‎ (13,442 views)
  249. Theory‏‎ (13,437 views)
  250. BE Classroom Computer Documentation‏‎ (13,430 views)

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