Assignment 4: finding and measuring things

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20.309: Biological Instrumentation and Measurement

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Overview

Congratulations on completing assignment 3. Now you can say you made an epifluorescence microscope. How cool is that?

The in-lab part of this assignment is straightforward. You will make a fluorescent image of tiny microspheres for measuring resolution and then you will record a three-minute movie of larger microspheres for measuring the noise in position measurements made with your microscope. Easy. If your microscope is in shape, you ought to be able to get the lab part done in well under an hour. (The tiny microspheres do not produce very much light. Sometimes people struggle a bit to get them in focus. Hint: make sure your microscope is well-adjusted before you look at really tiny, dim things.)

You will also develop image processing code to analyze the images. Using the image of tiny microspheres, you will measure the resolution of your microscope. You will use the movie of larger microspheres to assess how precisely position can be measured. Developing the code will take longer than the in-lab part of this assignment.

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