20.109(F22):Fall 2022 schedule

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20.109(F22): Laboratory Fundamentals of Biological Engineering

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Fall 2022 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Genomic instability        M2: Drug discovery        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sep 8/9 NLL
Lecture slides
Orientation and laboratory tour
T/R prelab
W/F prelab
M1D1 T/W Sep 13/14 BPE
Lecture slides
Learn best practices for mammalian cell culture
T/R prelab
W/F prelab corrected
Orientation quiz
Homework due
M1D2 R/F Sep 15/16 BPE
Lecture slides
Prepare and treat cells for γ-H2AX experiment
T/R prelab
W/F prelab
Homework due
M1D3 T/W Sep 20/21 BPE
Lecture slides
Use immunoflourescence staining to assess γ-H2AX experiment
T/R prelab
W/F prelab
Homework due
R/F Sep 22/23 BE Comm Lab Career Day holiday
M1D4 T/W Sep 27/28 BPE
Lecture slides
Complete data analysis for γH2AX experiment
T/R prelab
Laboratory quiz
Homework due
M1D5 R/F Sep 29/30 BPE
Treat cells for CometChip assay
Homework due
Research talk due Sat, Oct 1 at 10 pm
M1D6 T/W Oct 4/5 BPE
Image and analyze data for CometChip assay
Homework due
M1D7 R/F Oct 6/7 BPE
Examine experimental data using statistical methods
Laboratory quiz
Homework due
T/W Oct 11/12 Indigenous Peoples' Day holiday Data Summary draft due Wed, Oct 12 at 10 pm
Blog post due Thu, Oct 13 at 10 pm
M2D1 R/F Oct 13/14 JCN
Complete in-silico cloning of protein expression plasmid
Homework due
M2D2 T/W Oct 18/19 JCN
Perform protein purification protocol
Homework due
M2D3 R/F Oct 20/21 JCN
Assess purity and concentration of purified protein
Homework due
Data Summary revision due Sat, Oct 22 at 10 pm
M2D4 T/W Oct 25/26 JCN
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Laboratory quiz
Homework due
M2D5 R/F Oct 27/28 JCN
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Homework due
T/W Nov 1/2 Journal article presentations
R/F Nov 3/4 Journal article presentations Blog post due Sat, Nov 5 at 10 pm
M2D6 T/W Nov 8/9 JCN
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Homework due
R/F Nov 10/11 Veterans' Day holiday
M2D7 T/W Nov 15/16 JCN
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Laboratory quiz
Homework due
M3D1 R/F Nov 17/18 NLL
Brainstorm ideas for Research proposal presentation
Homework due
T/W Nov 22/23 Thanksgiving Day holiday Research article due Mon, Nov 21 at 10 pm
Blog post due Tue, Nov 22 at 10 pm
R/F Nov 24/25 Thanksgiving Day holiday
T/W Nov 29/30 AB
Research proposal pitches
M3D2 R/F Dec 1/2 BE Comm Lab Develop ideas for Research proposal presentation
Homework due
M3D3 T/W Dec 6/7 NLL
Participate in Research proposal peer reviews
Homework due
R/F Dec 8/9 Research proposal presentations Blog post due Sat, Dec 10 at 10 pm
T Dec 13 Celebration lunch!