20.109(F19):Class data

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20.109(F19): Laboratory Fundamentals of Biological Engineering

Fa19 20109 Banner image.png

Fall 2019 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Testing chemical probes              

Module 1: Measuring genomic instability

M1D5

gamma-H2AX analysis

Team Upload, then link the completed excel spreadsheet from the wiki here!
TR Orange Orange Team Data
TR Yellow Yellow Team Data
TR Green Green Team Data
TR Blue Blue Team Data
TR Pink Pink Team Data
TR Purple Purple Team Data
WF Cyan WF Team Data

M1D6

Comet Chip analysis

Team
TR Orange Orange Team Data
TR Yellow Yellow Team Data
TR Green Green Team Data
TR Blue Blue Team Data
TR Pink Pink Team Data
TR Purple Purple Team Data
WF Cyan WF Team Data

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand Colorimetric Assay Results
TR orange E ldhA GTACTGTTTTGTGCTATAAA Coding region Non-template Orange Team Data
TR yellow E ldhA AAATTCCAGCTCAAAGCCAAAGG Coding region Non-template Yellow Team Data
TR green E ack TTAGCCACGTATCAATTATAGG Promoter Region Template Green Team Data
TR blue A adhE ccagagcggcggcgcggaag Coding region Non-template Blue Team data
TR pink E ppc AGCATACTGACATTACTACGCAATG Coding region Non-Template Pink Team data
TR purple E ldhA CTTAAATGTGATTCAACATCACTGG Promoter region Template Purple Team Data

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA (DNA) sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand Colorimetric Assay Results
WF Cyan E frd GTGGGATAAAAACAATCTGG promoter Template WF FRD DATA ATHENA
WF Cyan A ppc ACTGACATTACTACGCAATG beginning of gene Non-template WF PPC Data
WF Cyan E pta TTTGTAACCCGCCAAATCGG promoter Template WF pta data
File:TR Yeloow Western.xlsx