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• DNA Melting Part 1: Measuring Temperature and Fluorescence
  ...he black lead of the top device should be connected to the red lead of the bottom device. ...to the red lead of the top TEC and the black wire to the black lead on the bottom TEC using wire nuts.
  27 KB (4,497 words) - 17:49, 19 April 2017
• DNA Melting Part 2: Lock-in Amplifier and Temperature Control
  ...to the red lead of the top TEC and the black wire to the black lead on the bottom TEC using wire nuts. # Connect the fan itself to the Fan +/- connections at the bottom of the PCB (red to +, black to -, innermost row. See figure).
  20 KB (3,227 words) - 20:05, 7 November 2017
• DNA Melting: Simulating DNA Melting - Intermediate Topics
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  9 KB (1,281 words) - 21:46, 17 August 2017
• Lab Manual: Optical Microscopy
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  23 KB (3,767 words) - 15:19, 10 January 2017
• Electronics primer
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  22 KB (3,534 words) - 13:50, 19 October 2018
• Lab Manual: Atomic Force Microscopy (AFM)
  ...12 &mu;m area, at a rate of one line per second, and is currently near the bottom of the image.</figure>]] ... the Y-scan direction tells the scanner whether to start at the top or the bottom of an image, and the trace/retrace selector determines whether each line is
  56 KB (9,320 words) - 20:42, 13 December 2017
• 20.109(S16):Induce protein expression (Day5)
  #*At the bottom right should be a link to download your sequencing results.
  16 KB (2,609 words) - 21:55, 23 February 2016
• Fall 2010 Syllabus
  |+ align="bottom" style="color:#e76700;" |''Food complements''
  266 B (28 words) - 00:57, 16 September 2010
• Procedure: Diffusion in biological gels
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• 20.345:Course Information
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  11 KB (1,722 words) - 17:22, 6 February 2018
• Spring 2011:Optical Trapping Lab
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  10 KB (1,595 words) - 16:29, 28 February 2012
• Confocal Wiki
  ...e the desired name of your image file (.tif) in the imwrite section at the bottom of the code.
  21 KB (3,664 words) - 21:38, 4 June 2015
• Theory
  ... (B, in electron/(pixel second)). The number of cycles is indicated at the bottom of each table. It has to be considered though that the number of cycles is
  9 KB (1,442 words) - 20:08, 19 May 2011
• Results
  If not stated at the bottom right corner of each image, the parameters used for the experiment were SNR
  2 KB (338 words) - 20:30, 19 May 2011
• DNA Melting: Processing DNA Melting Data
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  7 KB (1,108 words) - 17:58, 8 October 2011
• DNA Melting Report Requirements for Part 1
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  2 KB (393 words) - 21:34, 23 March 2017
• Status Report Interrogatories (Spring 2012)
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  4 KB (610 words) - 18:44, 13 August 2013
• Spring 2012:Leanna Morinishi
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  2 KB (249 words) - 17:50, 18 November 2013
• Spring 2012:Leanna Morinishi Lab 1
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  13 KB (2,020 words) - 19:56, 9 March 2012
• Spring 2012:Leanna Morinishi Journal
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  14 KB (2,366 words) - 04:50, 17 May 2012
• Spring 2012:Vincent Lee Journal
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  8 KB (1,373 words) - 18:43, 13 August 2013
• Spring 2012: Jonathan Gootenberg Journal
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  11 KB (1,937 words) - 22:57, 22 May 2012
• 20.345:Key Factors Table
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  2 KB (306 words) - 01:05, 21 March 2012
• Spring 2012:LFM
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  4 KB (576 words) - 05:19, 18 May 2012
• Understanding the lock-in amplifier
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  15 KB (1,965 words) - 16:36, 13 November 2017
• Optical Microscopy Part 4: Particle Tracking
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  9 KB (1,469 words) - 01:01, 10 March 2016
• Protocols for cell culture
  * Water at the bottom: * Blower always on, vented at the bottom (20.109 students, beware: this type of hood is different from regular ones
  18 KB (2,896 words) - 18:17, 25 February 2024
• Protocols
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  4 KB (644 words) - 18:24, 15 August 2013
• Optical Microscopy Part 1: Brightfield Microscopy
  .... Also, note that the stages are very expensive; always lift them from the bottom. ... Week 1 report: (top) Air Force target, (center) Silica spheres and dust, (bottom) Ronchi Ruling]]
  23 KB (3,758 words) - 22:45, 12 January 2017
• Aligning the optical trap
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  4 KB (645 words) - 22:09, 3 May 2013
• Optical Microscopy Part 2: Fluorescence Microscopy
  ...he day prior to the fluorescence imaging, cells were plated on 35 mm glass-bottom cell culture dishes (MatTek, equipped with coverslip suited for optical mic ...ple images and histogram. Top: fluorescent beads; center: reference slide, bottom: image histogram.]]
  20 KB (3,262 words) - 22:06, 14 August 2017
• Optical detectors, noise, and the limit of detection
  ===Bottom line on optical detector noise=== {{Template:20.309 bottom}}
  14 KB (2,102 words) - 13:42, 3 March 2021
• Error analysis
  ...ial configuration to be as insensitive as possible to such effects" </ref> Bottom line: systematic errors are much harder to characterize than random errors. ...g the subject of measurement error seem more mysterious than it really is. Bottom line: Precision quantifies the variability of a measurement. Accuracy speci
  24 KB (3,757 words) - 14:57, 3 September 2018
• Optics Bootcamp
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  28 KB (4,857 words) - 20:09, 31 July 2017
• 20.109(S08):Testing cell viability (Day3)
  ... over (to the right). This is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis. #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl
  18 KB (3,087 words) - 16:04, 15 June 2015
• 20.109(S08):Initiate cell culture (Day2)
  ...n body inside this sterile area. (By back wall I mean what will become the bottom inside wall when you lay the flask down, which is the surface on which your
  12 KB (2,006 words) - 16:04, 15 June 2015
• 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  27 KB (3,752 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Clean and cut DNA (Day 2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  10 KB (1,692 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,806 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,704 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  10 KB (1,634 words) - 14:24, 5 June 2015
• 20.109(S08):Site-directed mutagenesis (Day2)
  (*)<small>In a 14 mL polypropylene round-bottom tube, 1 &mu;L of DNA will be added to 50 &mu;L of competent cells, and the
  12 KB (1,954 words) - 14:24, 5 June 2015
• 20.109(S08):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  22 KB (3,631 words) - 14:24, 5 June 2015
• 20.109(S07):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  22 KB (3,724 words) - 15:32, 15 June 2015
• 20.109(S07): Start-up expression engineering
  ... sequence and whether you have the "top" strand as you did before, or the "bottom" strand as you will need for this reverse primer. [[Image:Macintosh HD-User
  28 KB (4,311 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 3
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  12 KB (2,079 words) - 15:32, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 2/Day 2
  7. Wipe the top and bottom sensor with a water soaked Chemwipe.<br> 8. Dry the top and bottom sensor with a dry Chemwipe.<br>
  19 KB (3,069 words) - 15:48, 15 June 2015
• 20.109(S07):Bio-material engineering/Screening library
  9. After panning for 30 minutes, move the gold slides to the bottom three wells of the six well dish with 2 ml fresh Gal Blocking/Binding Buffe
  9 KB (1,473 words) - 15:31, 15 June 2015
• Bryan Hernandez/20.109/Lab notebook/Module 1/Day 4
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  11 KB (1,751 words) - 15:31, 15 June 2015
• 20.109(S07):Start-up genome engineering
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example the recognition sequence for EcoRI is ...and give it a quick spin in the microfuge to bring any pellets down to the bottom of the eppendorf tube (be sure to balance your tube against another in the
  32 KB (5,419 words) - 15:31, 15 June 2015
• 20.109(S07): Start-up signal measurement
  ...e chosen a PDB ID (singular or plural), go back to the center panel at the bottom of the Protein Explorer front door and type this code into the box associat
  21 KB (3,477 words) - 15:31, 15 June 2015
• 20.109(S07): Measuring calcium in vitro
  7. Wipe the top and bottom sensor with a water soaked Chemwipe.<br> 8. Dry the top and bottom sensor with a dry Chemwipe.<br>
  20 KB (3,237 words) - 15:45, 15 June 2015
• 20.109(S07): Calcium signaling in vivo
  ...e, top plot), and the fractional saturation of the calcium indicator (red, bottom plot). If you were doing actual fluorescence experiments, the signal chang
  14 KB (2,275 words) - 15:31, 15 June 2015
• 20.109(F07): siRNA design
  ...equence from the MSWord document you started into the box that is near the bottom of the webpage. Delete any numbers once you’ve pasted the sequence. Choos ...u submit your query, several target sequences will appear as a list at the bottom of the webpage. The candidate sequences are listed according to where they
  21 KB (3,532 words) - 15:54, 15 June 2015
• 20.109(F07):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  22 KB (3,670 words) - 15:54, 15 June 2015
• 20.109(F07): DNA ligation and bacterial transformation
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  16 KB (2,748 words) - 15:54, 15 June 2015
• 20.109 (F07): Phage by design
  ...s being used for both bottom up and top down re-engineering of cells. In a bottom up approach, the genetic components for a “minimal cell” would be speci
  17 KB (2,797 words) - 15:54, 15 June 2015
• 20.109(F08):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  26 KB (4,345 words) - 19:05, 28 July 2015
• 20.109(F08): Phage by design
  ...s being used for both bottom up and top down re-engineering of cells. In a bottom up approach, the genetic components for a “minimal cell” would be speci
  17 KB (2,797 words) - 19:05, 28 July 2015
• 20.109(F08): Start-up expression engineering
  ... sequence and whether you have the "top" strand as you did before, or the "bottom" strand as you will need for this reverse primer. [[Image:Macintosh HD-User
  29 KB (4,451 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/Clean and cut DNA (Day 2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  10 KB (1,692 words) - 19:05, 28 July 2015
• 20.109(F08): Probe Western
  ...09" to login (Wed/Fri section: use "astachow" and "be109" instead). At the bottom of the left panel should be a link to download your sequencing results. Sel
  14 KB (2,285 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA engineering by PCR (Day 1)
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  23 KB (3,892 words) - 19:30, 28 July 2015
• 20.109(F08):DNA engineering/Agarose gel electrophoresis (Day 3)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,806 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/Examine candidate clones (Day 5)
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  10 KB (1,634 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,704 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  10 KB (1,702 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  22 KB (3,807 words) - 19:29, 28 July 2015
• 20.109(F08): Mod 1 Day 5 Examine candidate clones
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  11 KB (1,770 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,617 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  11 KB (1,817 words) - 19:05, 28 July 2015
• 20.109(F08): Mod 2 Day 1 Protein engineering with PCR
  ...e "landing" sequence that will annealing to the TAP-TRP fusion during PCR. Bottom line: limitations in the synthesis technology impose the 59 base limit, but ...Finally, add 39 bases downstream in the custom retrieval box that's on the bottom left of the page. Don't choose reverse complement if you plan to follow the
  30 KB (4,904 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 2 Day 2 Yeast transformation
  ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is ready to be checked on a gel and also transformed. ... to mix the contents. This will help keep the cells from settling to the bottom of the tube.
  18 KB (3,152 words) - 19:06, 28 July 2015
• 20.109(F08): Mod 2 Day 5 Probe western, isolate RNA
  ...ty. Pour the beads into the tube to a height of ~2.5 cm (~1 inch) from the bottom of the tube. See the picture here as a guide. Take the tubes and their caps
  20 KB (3,266 words) - 19:06, 28 July 2015
• 20.109(F09): Mod 2 Day 3 Tools for system engineering
  #Tap the cuvette on the bench so the cells rest in the bottom of the cuvette.
  24 KB (3,897 words) - 19:39, 28 July 2015
• 20.109(S09):Start-up protein engineering (Day1)
  ...atoms, click on ''More Views'' in the control panel, or on ''Jmol'' at the bottom right of the image panel. For example, you can highlight specific amino aci
  23 KB (3,877 words) - 19:33, 28 July 2015
• 20.109(S09):Prepare expression system (Day4)
  ...P1000, taking care not to disturb the pellet of plasmid DNA that is at the bottom of the tube, and put them in a 15 mL conical waste collection tube. Remove ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  21 KB (3,468 words) - 19:33, 28 July 2015
• 20.109(S09):Induce protein and evaluate DNA (Day5)
  ...ogin to dnaLIMS" link and then use "astachow" and "be109" to login. At the bottom of the left panel should be a link to download your sequencing results. Fir
  17 KB (2,822 words) - 19:33, 28 July 2015
• 20.109(S09):Assay protein behavior (Day7)
  ...ing fluid, pipet '''''slowly''''' while touching the pipet tip against the bottom or side of the well.
  7 KB (1,202 words) - 19:33, 28 July 2015
• 20.109(S09): siRNA design (Day1)
  ...equence from the MSWord document you started into the box that is near the bottom of the webpage. Delete any numbers once youve pasted the sequence. Choos ...u submit your query, several target sequences will appear as a list at the bottom of the webpage. The candidate sequences are listed according to where they
  22 KB (3,613 words) - 19:33, 28 July 2015
• 20.109(F09): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  22 KB (3,783 words) - 19:44, 28 July 2015
• 20.109(F09): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  11 KB (1,854 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,743 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,761 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 5 Examine candidate clones
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  11 KB (1,830 words) - 19:38, 28 July 2015
• 20.109(F09):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three eppendorfs. Using your P1000, add water to bring the final volume ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more eppendorfs. Using your P1000, add water to bring the final vo
  27 KB (4,508 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 2 Day 6 Readouts of DNA, Protein
  ...09" to login (Wed/Fri section: use "astachow" and "be109" instead). At the bottom of the left panel should be a link to download your sequencing results. Sel
  13 KB (2,115 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 2 Day 1 Testing an engineered biological system
  ...action to plastic cuvettes. '''Avoid the chloroform''' that will be at the bottom of your tubes. If you add the chloroform to the cuvettes, it will "etch" th
  13 KB (2,163 words) - 19:38, 28 July 2015
• 20.109(F10): Mod 2 Day 1 Testing an engineered biological system
  ...action to plastic cuvettes. '''Avoid the chloroform''' that will be at the bottom of your tubes. If you add the chloroform to the cuvettes, it will "etch" th
  14 KB (2,180 words) - 21:00, 28 July 2015
• 20.109(F10):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three eppendorfs. Using your P1000, add water to bring the final volume ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more eppendorfs. Using your P1000, add water to bring the final vo
  27 KB (4,538 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the D32N-fwd primer 5- at the bottom of your MSWord document. There are several things to consider as you design
  22 KB (3,783 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5 to 3 on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  12 KB (1,935 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,744 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  11 KB (1,791 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 5 Examine candidate clones
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  11 KB (1,830 words) - 21:00, 28 July 2015
• 20.109(S11):Purify aptamer-encoding DNA (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  11 KB (1,852 words) - 21:05, 28 July 2015
• 20.109(S11):Purify RNA and run affinity column (Day4)
  #Snap off the plastic tab at the bottom of the column (a little buffer may flow out), and place the column in the 2 #Snap the bottom off of each column. Save the top and bottom caps for later use.
  12 KB (2,130 words) - 21:05, 28 July 2015
• 20.109(S11):RNA to DNA by RT-PCR (Day5)
  ...d-curve.jpg|thumb|left|300px|'''Sample heme binding curves.''' From top to bottom, the curves are 8-12, a mixture of 6-5 and 8-12, 6-5 &mdash; each with heme
  8 KB (1,243 words) - 21:05, 28 July 2015
• 20.109(S11):Complete DNA design (Day2)
  ...g and palindromic, that is, they read the same 5 to 3 on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ...gn by specifying two synthetic oligonucleotides &mdash; a top strand and a bottom strand &mdash; that can be annealed together and then ligated directly into
  14 KB (2,340 words) - 21:05, 28 July 2015
• 20.109(S11):Prepare DNA for cloning (Day3)
  ...or the spectrophotometer, you can begin by picking up fourteen 14 mL round-bottom tubes and preparing sticky labels for your samples (described in step 6). #Distribute 2.5 mL of cells to each round-bottom tube with a 5 or 10 mL serological pipet.
  17 KB (2,967 words) - 21:05, 28 July 2015
• 20.109(F11): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,802 words) - 21:07, 28 July 2015
• 20.109(F11): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the D32N-fwd primer 5- at the bottom of your MSWord document. There are several things to consider as you design
  22 KB (3,809 words) - 21:08, 28 July 2015
• 20.109(F11):Guidelines for working in the tissue culture facility
  ...ter incubator. Swab them with 70% ethanol, especially at the neck and the bottom, and place them directly into the hood. Avoid shaking them vigorously duri ...pette from its wrapper at the center of the work area, tilt it so the tip (bottom end) is pointing away from the frontal non-sterile area and away from other
  10 KB (1,639 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5 to 3 on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  9 KB (1,486 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  12 KB (1,904 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 5 Examine candidate clones & tissue culture
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  13 KB (2,328 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 2 Day 1 Testing an engineered biological system
  ...action to plastic cuvettes. '''Avoid the chloroform''' that will be at the bottom of your tubes. If you add the chloroform to the cuvettes, it will "etch" th
  14 KB (2,184 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 2 Day 3 Tools for system engineering
  #Tap the cuvette on the bench so the cells rest in the bottom of the cuvette. ... at the top of the breadboard, and the ground wire to the blue rail at the bottom. (Power off the breadboard before you continue building, by disconnecting t
  30 KB (4,934 words) - 21:08, 28 July 2015
• 20.109(S13):Testing cell viability (Day3)
  #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl ...er to FITC (position 3). You should see a blue light coming from the bottom part of the microscope.
  15 KB (2,447 words) - 13:48, 29 July 2015
• 20.109(S13):Design mutant (Day1)
  ...atoms, click on ''More Views'' in the control panel, or on ''Jmol'' at the bottom right of the image panel. For example, you can highlight specific amino aci
  26 KB (4,387 words) - 13:48, 29 July 2015
• 20.109(S13):Prepare expression system (Day4)
  ...P1000, taking care not to disturb the pellet of plasmid DNA that is at the bottom of the tube, and put them in a 15 mL conical waste collection tube. Remove
  20 KB (3,344 words) - 13:48, 29 July 2015
• 20.109(S13):Induce protein and evaluate DNA (Day5)
  ...ogin" link and then use "astachow@mit.edu" and "be20109" to log in. At the bottom right should be a link to download your sequencing results.
  15 KB (2,584 words) - 13:48, 29 July 2015
• 20.109(S13):Purify protein (Day6)
  #Snap off the bottom of the column, place in a 15 mL conical tube, and loosen the column's cap.
  13 KB (2,134 words) - 13:48, 29 July 2015
• 20.109(S13):Preparing cells for analysis (Day4)
  ... plate. This transfer step is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis.
  25 KB (4,196 words) - 13:48, 29 July 2015
• 20.109(S13):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,582 words) - 13:48, 29 July 2015
• 20.109(S14):Preparing cells for analysis (Day4)
  ... plate. This transfer step is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis.
  24 KB (4,100 words) - 13:59, 29 July 2015
• 20.109(S14): TA notes for module 2
  ***Gel chambers set up &ndash; make sure to remove strip from bottom!
  8 KB (1,340 words) - 13:59, 29 July 2015
• 20.109(F14): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  13 KB (2,248 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  23 KB (3,882 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  11 KB (1,780 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  11 KB (1,836 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... pellets. After each vortexing step, the liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  20 KB (3,455 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 6 Lipofection and paper discussion
  #The next day gels were rinsed with PBS and then adhered to the bottom of a gel electrophoresis box using double sided tape.
  11 KB (1,850 words) - 14:02, 29 July 2015
• 20.109(F14):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.05% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.05% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  29 KB (4,864 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 2 Day 1 Testing an engineered biological system
  ...action to plastic cuvettes. '''Avoid the chloroform''' that will be at the bottom of your tubes. If you add the chloroform to the cuvettes, it will "etch" th
  15 KB (2,383 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 4 DNA ligations, bacterial transformations and CometChip
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  12 KB (2,060 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 2 Day 3 Tools for system engineering
  #Tap the cuvette on the bench so the cells rest in the bottom of the cuvette.
  28 KB (4,560 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 3 Day 2 Phage nanowires
  ... the last few drops but not the stir bar using a larger magnet held to the bottom of the inverted flask (see example image with jar) ...tes. At the end of this spin you should see dark material collected at the bottom of the tube. This is the material that will serve as the photoanode in our
  13 KB (2,165 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 4 DNA Ligation & Transformation
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  12 KB (2,028 words) - 14:02, 29 July 2015
• 20.109(S15): Complete Western and prepare damaged DNA (Day3)
  ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tubes is your purified, digested DNA.
  18 KB (2,985 words) - 17:15, 29 July 2015
• 20.109(S15): TA notes for module 2
  ***Gel chambers set up &ndash; make sure to remove strip from bottom!
  8 KB (1,328 words) - 17:15, 29 July 2015
• 20.109(S15):AIV detection assay and analysis (Day7)
  #At the bottom right should be a section called ''Recent Result''s. Click on ''More'' to e
  24 KB (3,931 words) - 17:15, 29 July 2015
• 20.109(S15):Begin Western protein analysis and choose system conditions (Day2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' (see als #Collect the cells to the bottom of the well by scraping each well with a '''fresh''' cell scraper.
  27 KB (4,480 words) - 17:15, 29 July 2015
• 20.109(S15):Biotemplating on phage nanowires (Day 2)
  ... the last few drops but not the stir bar using a larger magnet held to the bottom of the inverted flask (see example image with jar) ...tes. At the end of this spin you should see dark material collected at the bottom of the tube. This is the material that will serve as the photoanode in our
  14 KB (2,281 words) - 17:15, 29 July 2015
• 20.109(S15):Cell preparation for DNA repair assays (Day4)
  ...p half of a 24-well plate.)''' The top row will receive mixture A, and the bottom row mixture B. Each condition will be done in duplicate.]]
  18 KB (2,885 words) - 17:15, 29 July 2015
• 20.109(S15):DNA cloning (Day4)
  ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be loaded into a gel fo ...quick spin them in the microfuge to bring the contents of the tubes to the bottom.
  16 KB (2,696 words) - 17:15, 29 July 2015
• 20.109(S15):DNA repair assays (Day5)
  #*As you work, tip the plate down a little to pool the media at the bottom of each well. #*Place your aspirator at the bottom of a well, and suck up the media. Remove all the liquid, but remove the asp
  14 KB (2,396 words) - 17:15, 29 July 2015
• 20.109(S15):Introduction to cell strains and plating (Day1)
  ...r, as shown to you by the teaching faculty. Keep K1 on top and xrs6 on the bottom, so you don't forget which is where. [[Image:Be109cellcounting.jpg|thumb|ri ...e new 15 mL conical. Pay attention to see if the cells have settled to the bottom of the conical while you were counting.
  18 KB (3,046 words) - 17:15, 29 July 2015
• 20.109(S15):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.05% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.05% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  25 KB (4,176 words) - 17:15, 29 July 2015
• 20.109(S12):Purify aptamer-encoding DNA (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  11 KB (1,844 words) - 17:30, 29 July 2015
• 20.109(S12):Purify RNA and run affinity column (Day4)
  #Snap off the plastic tab at the bottom of the column (a little buffer may flow out), and place the column in the 2 #Snap the bottom off of each column. Save the top and bottom caps for later use.
  14 KB (2,442 words) - 17:30, 29 July 2015
• 20.109(S12):RNA to DNA by RT-PCR (Day5)
  ...d-curve.jpg|thumb|left|300px|'''Sample heme binding curves.''' From top to bottom, the curves are 8-12, a mixture of 6-5 and 8-12, 6-5 &mdash; each with heme
  8 KB (1,365 words) - 17:30, 29 July 2015
• 20.109(S12):Testing cell viability (Day3)
  #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl ...er to FITC (position 3). You should see a blue light coming from the bottom part of the microscope.
  17 KB (2,877 words) - 17:30, 29 July 2015
• 20.109(S12):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,554 words) - 17:30, 29 July 2015
• 20.109(S12):Start-up protein engineering (Day1)
  ...atoms, click on ''More Views'' in the control panel, or on ''Jmol'' at the bottom right of the image panel. For example, you can highlight specific amino aci
  26 KB (4,263 words) - 17:30, 29 July 2015
• 20.109(S12):Prepare expression system (Day4)
  ...P1000, taking care not to disturb the pellet of plasmid DNA that is at the bottom of the tube, and put them in a 15 mL conical waste collection tube. Remove
  19 KB (3,278 words) - 17:30, 29 July 2015
• 20.109(S12):Induce protein and evaluate DNA (Day5)
  ...ogin" link and then use "astachow@mit.edu" and "be20109" to log in. At the bottom right should be a link to download your sequencing results. Select order da
  17 KB (2,911 words) - 17:30, 29 July 2015
• 20.109(S12):Characterize protein expression (Day6)
  #Snap off the bottom of the column, place in a 15 mL conical tube, and loosen the column's cap.
  13 KB (2,041 words) - 17:30, 29 July 2015
• 20.109(S12):Transcript-level analysis (Day6)
  #*The top 1-3 samples in the standards may become bright yellow, while the bottom 1-2 samples may appear very pale yellow. As with the &beta;-gal assay, we h
  17 KB (2,752 words) - 17:30, 29 July 2015
• 20.109(S12): RNA computational analysis
  ...t the “Show as Phylogram Tree” option. You will need to scroll to the bottom of the page to see the results of this operation. You can always go back t
  12 KB (1,846 words) - 17:30, 29 July 2015
• 20.109(S12):Preparing cells for analysis (Day4)
  ... plate. This transfer step is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis.
  19 KB (3,261 words) - 17:30, 29 July 2015
• 20.109(F13)
  *Thank you, Tom, for adding previous/next day navigation links to the bottom of each Mod 2 wiki page! Folks, please let us know how these work out and i
  7 KB (1,152 words) - 17:33, 29 July 2015
• 20.109(F13):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.05% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.05% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  28 KB (4,787 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  21 KB (3,687 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  11 KB (1,881 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  11 KB (1,914 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  13 KB (2,222 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 1 Day 5 Examine candidate clones & tissue culture
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... pellets. After each vortexing step, the liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  17 KB (2,866 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 3 Day 2 Phage nanowires
  ... the last few drops but not the stir bar using a larger magnet held to the bottom of the inverted jar (see image) ...tes. At the end of this spin you should see dark material collected at the bottom of the tube. This is the material that will serve as the photoanode in our
  15 KB (2,422 words) - 17:33, 29 July 2015
• 20.109(F13): Mod 2 Day 4 Phosphotyrosine Western Blot Analysis
  # Collect the cells to the bottom of the well by scraping each well with the cell scraper. ...f tubes and keep on ice '''be careful not to disturb the pellet at the bottom!'''
  20 KB (3,082 words) - 17:33, 29 July 2015
• 20.109(S10):Purify aptamer-encoding DNA (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  11 KB (1,764 words) - 19:49, 28 July 2015
• 20.109(S10):Purify RNA and run affinity column (Day4)
  #Snap off the plastic tab at the bottom of the column (a little buffer may flow out), and place the column in the 2 #Snap the bottom off of each column. Save the top and bottom caps for later use.
  12 KB (2,127 words) - 19:49, 28 July 2015
• 20.109(S10):Start-up protein engineering (Day1)
  ...atoms, click on ''More Views'' in the control panel, or on ''Jmol'' at the bottom right of the image panel. For example, you can highlight specific amino aci
  23 KB (3,840 words) - 19:49, 28 July 2015
• 20.109(S10):Prepare expression system (Day4)
  ...P1000, taking care not to disturb the pellet of plasmid DNA that is at the bottom of the tube, and put them in a 15 mL conical waste collection tube. Remove ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
  22 KB (3,701 words) - 19:49, 28 July 2015
• 20.109(S10):Induce protein and evaluate DNA (Day5)
  ...ogin to dnaLIMS" link and then use "astachow" and "be109" to login. At the bottom of the left panel should be a link to download your sequencing results. Fir
  17 KB (2,849 words) - 19:49, 28 July 2015
• 20.109(S10):Assay protein behavior (Day7)
  ...ing fluid, pipet '''''slowly''''' while touching the pipet tip against the bottom or side of the well.
  7 KB (1,187 words) - 19:49, 28 July 2015
• 20.109(S10):Lab tour
  ...our P20, measure 10, 15 and 20 μl of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μl of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  26 KB (4,410 words) - 19:49, 28 July 2015
• 20.109(S10):Testing cell viability (Day3)
  #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl ...FITC (position 3). You should see a blue light coming from the bottom part of the microscope.
  16 KB (2,677 words) - 22:02, 28 August 2016
• 20.109(S10):Preparing cells for analysis (Day4)
  ... plate. This transfer step is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis.
  15 KB (2,534 words) - 19:49, 28 July 2015
• 20.109(S11):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  26 KB (4,424 words) - 21:05, 28 July 2015
• 20.109(S11): RNA computational analysis
  ...t the Show as Phylogram Tree option. You will need to scroll to the bottom of the page to see the results of this operation. You can always go back t .../mfold.bioinfo.rpi.edu/ Mfold homepage] and in the cited literature at the bottom of that page.
  12 KB (1,829 words) - 21:05, 28 July 2015
• 20.109(F12): Mod 1 Day 5 Examine candidate clones & tissue culture
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  13 KB (2,328 words) - 13:41, 29 July 2015
• 20.109(F12):Guidelines for working in the tissue culture facility
  ...ter incubator. Swab them with 70% ethanol, especially at the neck and the bottom, and place them directly into the hood. Avoid shaking them vigorously duri ...pette from its wrapper at the center of the work area, tilt it so the tip (bottom end) is pointing away from the frontal non-sterile area and away from other
  10 KB (1,639 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 1 DNA engineering using PCR
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  22 KB (3,714 words) - 13:41, 29 July 2015
• 20.109(F12):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three eppendorfs. Using your P1000, add water to bring the final volume ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more eppendorfs. Using your P1000, add water to bring the final vo
  27 KB (4,585 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 3 Day 2 Phage nanowires
  ... the last few drops but not the stir bar using a larger magnet held to the bottom of the inverted jar (see image) ...tes. At the end of this spin you should see dark material collected at the bottom of the tube. This is the material that will serve as the photoanode in our
  10 KB (1,590 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 2 Clean and cut DNA
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  9 KB (1,570 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,758 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 1 Day 4 DNA ligations and bacterial transformations
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  12 KB (1,926 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 2 Day 1 Testing an engineered biological system
  ...action to plastic cuvettes. '''Avoid the chloroform''' that will be at the bottom of your tubes. If you add the chloroform to the cuvettes, it will "etch" th ...up for a speaking slot on M2D4 or M2D8. A sign up list can be found at the bottom of [[20.109(F12):Guidelines for oral presentations| this page.]] Even if yo
  15 KB (2,366 words) - 13:41, 29 July 2015
• 20.109(F12): Mod 2 Day 3 Tools for system engineering
  #Tap the cuvette on the bench so the cells rest in the bottom of the cuvette. ... at the top of the breadboard, and the ground wire to the blue rail at the bottom. (Power off the breadboard before you continue building, by disconnecting t
  30 KB (4,879 words) - 13:41, 29 July 2015
• 20.109(S13):Transcript-level analysis (Day6)
  #*The top 1-3 samples in the standards may become bright yellow, while the bottom 1-2 samples may appear very pale yellow. Once again, we have a signal:noise
  17 KB (2,748 words) - 13:47, 29 July 2015
• 20.109(S13):DNA cloning (Day4)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  16 KB (2,679 words) - 13:47, 29 July 2015
• 20.109(S13):Phylogenetic analysis (Day7)
  #At the bottom right should be a section called ''Recent Result''s. Click on ''More'' to e ...ing a chunk of the vector) is returned. You should also scroll down to the bottom to check if any of your failed reactions were repeated; these are noted wit
  17 KB (2,753 words) - 13:48, 29 July 2015
• 20.109(S14):Phylogenetic and primer analyses (Day7)
  #At the bottom right should be a section called ''Recent Result''s. Click on ''More'' to e ...check whether you can locate an insert. You should also scroll down to the bottom of the Genewiz table to check if any of your failed reactions were repeated
  17 KB (2,730 words) - 13:58, 29 July 2015
• 20.109(S14):DNA cloning (Day4)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  15 KB (2,521 words) - 13:58, 29 July 2015
• 20.109(S14):Lab tour
  ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  28 KB (4,689 words) - 13:59, 29 July 2015
• 20.109(S14):Introduction to cell strains and plating (Day1)
  ...r, as shown to you by the teaching faculty. Keep K1 on top and xrs6 on the bottom, so you don't forget which is where. [[Image:Be109cellcounting.jpg|thumb|ri
  17 KB (2,764 words) - 13:59, 29 July 2015
• 20.109(S14):Begin Western protein analysis (Day2)
  #Collect the cells to the bottom of the well by scraping each well with a '''fresh''' cell scraper. ...go from top to bottom windshield wiper style to pool them down towards the bottom of the tilted dish.
  16 KB (2,680 words) - 13:59, 29 July 2015
• 20.109(S14):Complete Western and prepare damaged DNA (Day4)
  ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tubes is your purified, digested DNA.
  17 KB (2,797 words) - 13:59, 29 July 2015
• 20.109(S14):Cell preparation for DNA repair assays (Day5)
  ...p half of a 24-well plate.)''' The top row will receive mixture A, and the bottom row mixture B. Each condition will be done in duplicate.]]
  9 KB (1,535 words) - 13:59, 29 July 2015
• 20.109(S14):DNA repair assays(Day6)
  #*As you work, tip the plate down a little to pool the media at the bottom of each well. #*Place your aspirator at the bottom of a well, and suck up the media. Remove all the liquid, but remove the asp
  17 KB (2,912 words) - 13:59, 29 July 2015
• 20.109(S14):Testing cell viability (Day3)
  #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl ...er to FITC (position 3). You should see a blue light coming from the bottom part of the microscope.
  15 KB (2,507 words) - 13:59, 29 July 2015
• 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)
  ...atoms, click on ''More Views'' in the control panel, or on ''Jmol'' at the bottom right of the image panel. For example, you can highlight specific amino aci
  28 KB (4,525 words) - 14:45, 12 November 2015
• 20.109(F15):Agarose gel electrophoresis (Day3)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  11 KB (1,795 words) - 19:08, 23 September 2015
• 20.109(F15):Induce protein and evaluate DNA (Day3)
  #*At the bottom right should be a link to download your sequencing results.
  16 KB (2,649 words) - 20:22, 21 October 2015
• 20.109(F15):Purify protein (Day4)
  #For each of your two samples, snap off the bottom of a Zeba column, place in a 15 mL conical tube, and loosen the column's ca
  13 KB (2,001 words) - 18:01, 11 November 2015
• 20.109(F15):Lab tour
  ...our P20, measure 10, 15 and 20 μL of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μL of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  23 KB (3,828 words) - 19:49, 11 September 2015
• 20.109(F15):DNA engineering using PCR (Day1)
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  21 KB (3,638 words) - 13:47, 2 October 2015
• 20.109(F15):Clean and cut DNA (Day2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' (see als ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product (cleaned-up D32N-pCX-EGFP), ready
  11 KB (1,805 words) - 19:52, 18 September 2015
• 20.109(F15):DNA ligation & transformation (Day4)
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  11 KB (1,745 words) - 20:32, 24 September 2015
• 20.109(F15):Examine candidate clones & tissue culture (Day5)
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... pellets. After each vortexing step, the liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  21 KB (3,522 words) - 18:18, 30 September 2015
• 20.109(F15):Lipofection (Day6)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom.
  16 KB (2,572 words) - 17:58, 1 October 2015
• Manta G032 camera measurements
  {{Template:20.309 bottom}}
  5 KB (856 words) - 00:26, 22 October 2015
• SToNCalculator.m
  {{Template:20.309 bottom}}
  2 KB (271 words) - 18:45, 16 November 2015
• 20.109(S16):Lab tour
  ...our P20, measure 10, 15 and 20 μL of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μL of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  23 KB (3,828 words) - 13:50, 4 February 2016
• 20.109(S16):Introduction to cell strains and plating (Day1)
  #Retrieve your flasks from the incubator and firmly tap the bottom 5x to dislodge the cells. ...hown to you by the teaching faculty. Keep M059K on 'top' and M059J on the 'bottom', so you don't forget which is which. [[Image:Be109cellcounting.jpg|thumb|r
  16 KB (2,780 words) - 17:34, 19 September 2016
• 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)
  ...r pipet (be sure to cap the pasteur pipet with a clean yellow tip!) at the bottom of the well. ...is buffer to the top of each dish and allow it to run down the dish to the bottom.
  21 KB (3,393 words) - 15:00, 11 March 2016
• 20.109(S16):Complete Western and prepare damaged DNA (Day3)
  ... eppendorf tube to mix and use the centrifuge to collect the liquid at the bottom of the tube. ...ature for 5 min and then spin as before. The material that collects in the bottom of the eppendorf tube is your purified, digested DNA.
  16 KB (2,558 words) - 15:49, 1 April 2016
• 20.109(S16):Cell preparation for DNA repair assays (Day5)
  ... top row will receive mixture A (see below, intact pMAX_EGFP_MCS), and the bottom row mixture B (see below, damaged pMAX_EGFP_MCS). Each condition will be pr
  16 KB (2,542 words) - 18:34, 28 March 2016
• 20.109(S16):In situ cloning (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below ...ch tube. Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin down.
  22 KB (3,676 words) - 21:29, 4 February 2016
• 20.109(S16):Design mutation primers (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. [[Image:Mod1 2 agaro gel.jpg|thumb|right|200px|'''Illustration of proper g ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  23 KB (3,783 words) - 20:41, 9 February 2016
• Temporary Optics Bootcamp
  {{Template:20.309 bottom}}
  15 KB (2,559 words) - 21:32, 2 February 2016
• 20.109(S16):Purify protein (Day6)
  #For each of your two samples, snap off the bottom of a Zeba column, place in a 15 mL conical tube, and loosen the column's ca
  14 KB (2,249 words) - 20:15, 26 February 2016
• 20.109(S16): TA notes for module 2
  ***gel chambers set up &ndash; make sure to remove strip from bottom!
  7 KB (1,054 words) - 18:04, 16 March 2016
• 20.109(F16):Prepare comet chips (Day1)
  #*Note: you are writing on what will be the bottom of the CometChip and may want to write backwards so the labels are clear wh #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga
  12 KB (1,972 words) - 17:32, 19 September 2016
• 20.109(F16):DNA engineering using PCR (Day1)
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  21 KB (3,638 words) - 14:58, 18 May 2016
• 20.109(F16):Lab tour
  ...our P20, measure 10, 15 and 20 μL of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μL of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,439 words) - 21:13, 9 September 2016
• 20.109(F16):Clean and cut DNA (Day2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' (see als ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product (cleaned-up D32N-pCX-EGFP), ready
  11 KB (1,805 words) - 15:11, 18 May 2016
• 20.109(F16):Agarose gel electrophoresis (Day3)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  11 KB (1,791 words) - 15:16, 18 May 2016
• 20.109(F16):DNA ligation & transformation (Day4)
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  11 KB (1,745 words) - 15:25, 18 May 2016
• 20.109(F16):Examine candidate clones & tissue culture (Day5)
  ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... pellets. After each vortexing step, the liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds.
  21 KB (3,522 words) - 15:28, 18 May 2016
• 20.109(F16):Lipofection (Day6)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom.
  16 KB (2,572 words) - 15:33, 18 May 2016
• Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking
  {{Template:20.309 bottom}}
  16 KB (2,559 words) - 22:47, 15 March 2017
• 20.109(F16):Seed cells for immuno-flourescence assay (Day5)
  #*To ensure the coverslip settles at the bottom of the well, use a clean pipet tip to gently press down on the coverslip. #*Add 1 mL of trypsin to the flask and rock from side to side to ensure the bottom is coated, then incubate the flask in the 37&deg;C incubator for 2 min.
  12 KB (2,013 words) - 20:08, 30 September 2016
• 20.109(F16):Design gRNA for CRISPRi (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ...AA) sequence and nucleotide sequence (NT) of your gene are provided at the bottom of the page.
  13 KB (2,092 words) - 15:25, 6 October 2016
• Bode plots
  {{Template:20.309 bottom}}
  16 KB (2,729 words) - 20:05, 31 July 2017
• 20.109(F16):Complete in silico cloning (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below ...ch tube. Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin down.
  23 KB (3,824 words) - 17:24, 13 October 2016
• 20.109(F16):Asses DNA repair variability with comet chip (Day4)
  ... labels on the right refer to the Coriell cell lines and the labels on the bottom refer to length of time allotted for recovery following exposure to H<sub>2
  8 KB (1,390 words) - 17:23, 27 September 2016
• 20.109(S17):Lab tour
  ...our P20, measure 10, 15 and 20 μL of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μL of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  24 KB (3,951 words) - 21:35, 31 January 2017
• 20.109(S17):In silico cloning and induction of protein expression (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below
  24 KB (3,850 words) - 19:53, 2 March 2017
• Assignment 3, Part 1: visualizing actin with fluorescence contrast
  * Optical access? Check. We will grow the cells in a single layer in a glass-bottom culture dish. The cells are nearly transparent. Optical access is easy. * 2 glass bottom, 35 mm culture dishes with ~60% confluent 3T3 cells
  15 KB (2,365 words) - 20:25, 25 February 2020
• 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. #*Keep BRCA2- on the 'top' and DLD-1 on the 'bottom', so you don't forget which is which.
  20 KB (3,359 words) - 21:06, 9 March 2017
• 20.109(S17):Assess cell survival and harvest RNA for quantitative PCR assay (Day3)
  #Retrieve your flasks from the incubator and firmly tap the bottom 5 times to dislodge the cells. ... ensure you collect all of the cells in your flask, pipet the PBS down the bottom of the flask to wash the cells from the surface.
  13 KB (2,208 words) - 01:48, 9 February 2017
• 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. ... ensure you collect all of the cells in your flask, pipet the PBS down the bottom of the flask to wash the cells from the surface.
  15 KB (2,526 words) - 19:11, 30 October 2017
• 20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)
  #Click the 'Get Primers' button at the bottom of the screen.
  13 KB (2,158 words) - 17:55, 22 March 2017
• 20.109(F17):Lab tour
  ...our P20, measure 10, 15 and 20 μL of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μL of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  24 KB (3,974 words) - 13:25, 14 September 2017
• 20.109(F17):Prepare comet chips (Day1)
  #*Note: you are writing on what will be the bottom of the CometChip and may want to write backwards so the labels are clear wh #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga
  18 KB (2,935 words) - 20:42, 19 September 2017
• Assignment 1, Part 4: Measuring magnification and bead size
  ... Week 1 report: (top) Air Force target, (center) Silica spheres and dust, (bottom) Ronchi Ruling]]</figure> * The stages are very expensive. Always lift from the bottom.
  8 KB (1,209 words) - 04:16, 14 February 2020
• Assignment 1, Part 2: Optics bootcamp
  {{Template:20.309 bottom}}
  20 KB (3,371 words) - 06:40, 31 January 2020
• Assignment 1, Part 3: Building your transillumination microscope
  ...stranded inside a four-rod cage. It's usually best to eliminate one of the bottom rods so you can hang stuff on the top rods. You'll see why later. ...arger holes for 30mm cage rods. You'll use one of each. Use the C4W as the bottom-most mirror holder. The C6W will stack on top of the C4W, and will later ho
  38 KB (6,482 words) - 19:23, 6 September 2019
• Assignment 7, Part 2: measure temperature and fluorescence
  ...he black lead of the top device should be connected to the red lead of the bottom device. ...to the red lead of the top TEC and the black wire to the black lead on the bottom TEC using wire nuts.
  22 KB (3,650 words) - 17:42, 6 April 2018
• Assignment 10 Overview
  {{Template:20.309 bottom}}
  8 KB (1,286 words) - 16:37, 8 May 2018
• 20.109(F17):Complete in silico cloning (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below ...ch tube. Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin down.
  23 KB (3,835 words) - 19:34, 13 October 2017
• 20.109(F17):Design gRNA for CRISPRi (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ...AA) sequence and nucleotide sequence (NT) of your gene are provided at the bottom of the page.
  13 KB (2,115 words) - 20:47, 17 October 2017
• 20.109(F17):Evaluate cell loading results (Day3)
  #*Note: you are writing on what will be the bottom of the CometChip and may want to write backwards so the labels are clear wh #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga
  8 KB (1,360 words) - 20:41, 19 September 2017
• 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
  #**Lightly touch the tip to the bottom of each imprinted well on the CometChip and ''immediately'' lift the tip fr
  12 KB (1,986 words) - 21:55, 21 September 2017
• 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)
  #Use the 'hook' created in Step #8 to lift the coverslip from the bottom of the well, then use the tweezers to 'catch' the coverslip.
  15 KB (2,329 words) - 15:22, 4 October 2017
• Assignment 2 Part 2: Fluorescence microscopy
  ...ontal lines. The ground state of the molecule S₀ appears at the bottom of the diagram. Two excited states are shown: S₁ and S<sub>2</su ...ted by "T") have two unpaired spins. Don't sweat the details too much. The bottom line is that transitions ''within'' a column are much more likely than tran
  18 KB (2,752 words) - 18:39, 12 September 2019
• Assignment 2 Part 4: Fluorescent imaging of actin
  ...he day prior to the fluorescence imaging, cells were plated on 35 mm glass-bottom cell culture dishes (MatTek, equipped with coverslip suited for optical mic {{Template:20.309 bottom}}
  6 KB (946 words) - 03:21, 14 September 2017
• Assignment 2 Part 3: Build an epi-illuminator for your microscope
  #* Use the bottom mirror (not the dichroic) to center the illumination on the field of view o #* Finally, if necessary, readjust the bottom mirror's alignment to center the illumination on the field of view of the c
  13 KB (2,282 words) - 19:21, 21 February 2020
• 20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below #Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin down.
  23 KB (3,774 words) - 21:17, 16 February 2018
• 20.109(S18):Practice tissue culture techniques and prepare cells for RNA purification (Day1)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. #*Keep BRCA2-/- on the 'top' and DLD-1 on the 'bottom', so you don't forget which is which.
  13 KB (2,165 words) - 13:44, 20 March 2018
• 20.109(S18):Analyze small microarray data and induce protein expression (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. #*You can saturate the foreground fluorescence signal by moving the bottom slider leftward.
  17 KB (2,771 words) - 21:15, 16 February 2018
• 20.109(S18):Laboratory tour
  ...our P20, measure 10, 15 and 20 μL of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 μL of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  26 KB (4,362 words) - 21:39, 6 February 2018
• 20.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. ... ensure you collect all of the cells in your flask, pipet the PBS down the bottom of the flask to wash the cells from the surface.
  12 KB (1,963 words) - 00:38, 19 March 2019
• 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)
  #At a row on the bottom of column C, type in the following command: =INDEX($B$''FirstRow'':$B$''Las #Then, drag the bottom left corner of the cell across all relevant columns to apply the formula to
  11 KB (1,775 words) - 13:16, 8 March 2018
• 20.109(F18):Lab tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,435 words) - 19:28, 6 September 2018
• 20.109(F18):Complete in silico cloning (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below #Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin in the microcentrifuge.
  23 KB (3,819 words) - 13:51, 12 September 2019
• 20.109(F18):Design gRNA for CRISPRi (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ...AA) sequence and nucleotide sequence (NT) of your gene are provided at the bottom of the page.
  13 KB (2,112 words) - 15:29, 25 October 2018
• 20.109(F18):Practice tissue culture and prepare microwell array (Day1)
  #*Note: you are writing on what will be the bottom of the CometChip and may want to write backwards so the labels are clear wh #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga
  19 KB (3,096 words) - 18:35, 26 September 2018
• 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
  #**Lightly touch the tip to the bottom of each imprinted well on the CometChip and ''immediately'' lift the tip fr
  9 KB (1,587 words) - 22:12, 20 September 2018
• Electronics written problems
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  6 KB (975 words) - 18:45, 15 April 2019
• Electronics written problems II
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  5 KB (776 words) - 17:26, 23 January 2020
• 20.109(S19):Purify protein for secondary assays(Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ...p out of the column by gravitational flow, putting a stopper at the spout (bottom) of the column just before the PBS has completely flowed through the column
  13 KB (2,055 words) - 19:34, 25 February 2019
• 20.109(S19):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,454 words) - 15:43, 6 February 2019
• 20.109(S19):Purify RNA and practice RNA-seq data analysis methods (Day3)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. ... ensure you collect all of the cells in your flask, pipet the PBS down the bottom of the flask to wash the cells from the surface.
  12 KB (1,923 words) - 20:29, 16 April 2019
• 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below #Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin down.
  22 KB (3,675 words) - 19:34, 25 February 2019
• 20.109(S19):Evaluate protein purity and concentration (Day4)
  #Remove the bottom of the desalting column and place the column in a 15 mL conical tube with t ...ur desalted and concentrated FKBP12 protein into separate, wells of a flat bottom 96-well plate.
  12 KB (1,951 words) - 14:41, 22 February 2019
• 20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6)
  #At a row on the bottom of column C, type in the following command: =INDEX($B$''FirstRow'':$B$''Las #Then, drag the bottom left corner of the cell across all relevant columns to apply the formula to
  10 KB (1,695 words) - 20:09, 5 March 2019
• 20.109(S19):Practice tissue culture techniques and prepare cells for RNA purification (Day1)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. #*Keep BRCA2-/- on the 'top' and DLD-1 on the 'bottom', so you don't forget which is which.
  14 KB (2,344 words) - 20:32, 16 April 2019
• 20.109(F19):Lab tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,413 words) - 20:37, 30 August 2019
• 20.109(F19):Complete in silico cloning (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below #Flick the tubes to mix the contents then gather the liquid in the bottom of the tube with a short spin in the microcentrifuge.
  23 KB (3,785 words) - 22:22, 7 August 2019
• 20.109(F19):Design gRNA for CRISPRi (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ...AA) sequence and nucleotide sequence (NT) of your gene are provided at the bottom of the page.
  13 KB (2,121 words) - 13:56, 13 September 2019
• 20.109(F19):Treat cells for gamma-H2AX (Day2)
  #*Note: you are writing on what will be the bottom of the CometChip and may want to write backwards so the labels are clear wh #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga
  10 KB (1,755 words) - 19:12, 17 September 2019
• 20.109(F19):Complete in silico cloning of pdCas9 (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below #*Click 'Sequence' from the options at the bottom of the window.
  25 KB (4,089 words) - 12:11, 8 October 2019
• 20.109(F19):Design gRNA sequence for CRISPRi (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ...AA) sequence and nucleotide sequence (NT) of your gene are provided at the bottom of the page.
  13 KB (2,116 words) - 18:52, 14 August 2019
• 20.109(F19):Load cells into CometChip and apply treatments (Day4)
  #**Lightly touch the tip to the bottom of each imprinted well on the CometChip and ''immediately'' lift the tip fr
  12 KB (1,976 words) - 15:14, 24 September 2019
• 20.109(F19):Prepare for cellular thermal shift assay (Day1)
  #Retrieve your flask from the incubator and firmly tap the bottom to dislodge the cells. ...spension by pipetting before distributing. The cells likely settled to the bottom of the conical tube while you were working.
  14 KB (2,315 words) - 16:18, 7 November 2019
• 20.109(F19):Begin Western blot analysis (Day3)
  #Carefully open the iBlot transfer stack and separate the top stack from the bottom stack (see image to right for iBlot transfer stack setup).[[File:Fa19 iBlot #*Keep the bottom stack in the plastic tray.
  8 KB (1,366 words) - 20:06, 20 November 2019
• 20.109(S20):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  27 KB (4,434 words) - 14:55, 5 February 2020
• 20.109(S20):Assess purity and concentration of TDP43 protein (Day3)
  ... &mu;L of your purified TDP43-RRM12 protein into separate, wells of a flat bottom 96-well plate.
  12 KB (1,919 words) - 19:18, 27 February 2020
• 20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is below #*Click 'Sequence' from the options at the bottom of the window.
  26 KB (4,314 words) - 18:17, 7 February 2020
• 20.109(S20):Prepare cells for RNA purification and practice RNA-seq data analysis methods (Day1)
  #Retrieve your flasks from the incubator and firmly tap the bottom 10 times to dislodge the cells. ...the small volume of cells to the flasks as the cells likely settled to the bottom of the conical while you were counting.
  13 KB (2,111 words) - 21:39, 1 March 2020
• 20.109(S20):Purify RNA from etoposide-treated cells and and generate cDNA (Day3)
  #Retrieve your flask from the incubator and firmly tap the bottom 10 times to dislodge the cells. ... ensure you collect all of the cells in your flask, pipet the PBS down the bottom of the flask to wash the cells from the surface.
  10 KB (1,734 words) - 20:59, 28 February 2020
• 20.109(S20):Journal club presentations (Day4 and 5)
  #At the bottom of the window, click 'Share Screen' and select your Journal club presentati
  18 KB (2,824 words) - 21:51, 7 April 2020
• 20.109(S20):Purify TDP43 protein (Day2)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. #When the PBS has flowed through, cap the bottom and the top of the column until you are ready to add the cell lysate in Par
  14 KB (2,233 words) - 19:52, 12 February 2020
• 20.109(S20):Characterize clone by titration using flow cytometry (Day4)
  ...previous samples. Your samples will then be filtered one by one into round bottom analyzer tubes and placed under the cytometer sip. The machine will then su
  10 KB (1,617 words) - 19:11, 20 April 2020
• Spring 2020 Assignment 9
  {{Template:20.309 bottom}}
  11 KB (1,616 words) - 16:42, 30 April 2020
• 20.109(F20):M1D5
  #**Lightly touch the tip to the bottom of each imprinted well on the CometChip and ''immediately'' lift the tip fr ... v2.png|center|700px|thumb|'''Microscopy image of loaded CometChip.''' (A) Bottom of macrowell showing evenly spaced microwells. (B) Close-up of empty microw
  15 KB (2,489 words) - 22:13, 17 September 2020
• 20.109(F20):M1D6
  ... were treated with 20 &mu;M H₂O₂. The values at the bottom of the sheet are for the no H₂O₂ samples.
  13 KB (2,151 words) - 14:28, 14 September 2020
• 20.109(F20):M2D1
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  25 KB (4,057 words) - 18:52, 15 October 2021
• 20.109(F20):M2D3
  ... of your concentrated PF3D7_1351100 protein into separate, wells of a flat bottom 96-well plate.
  15 KB (2,402 words) - 21:45, 18 August 2020
• 20.109(F20):M3D5
  ...AA) sequence and nucleotide sequence (NT) of your gene are provided at the bottom of the page. #*Click the BLAST button at the bottom of the screen.
  8 KB (1,355 words) - 00:08, 31 July 2020
• 20.109(F20):M3D2
  ...AA) sequence and nucleotide sequence (NT) for the gene are provided at the bottom of the page.
  17 KB (2,660 words) - 23:58, 30 July 2020
• In silico cloning of pdCas9 construct
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. The DNA ends that result from restriction enzyme digests are
  19 KB (3,021 words) - 20:40, 3 August 2020
• 20.109(S21):M1D1
  #At the bottom of the screen switch to feature view. ...quick spin them in the microfuge to bring the contents of the tubes to the bottom.
  21 KB (3,368 words) - 20:14, 18 February 2021
• 20.109(S21):M1D6
  ...previous samples. Your samples will then be filtered one by one into round bottom analyzer tubes and placed under the cytometer sample injection port (SIP). #To clean the machine, place a round bottom tube with cleaning solution on the SIP and under Run settings, choose "Run
  14 KB (2,126 words) - 19:48, 17 March 2021
• 20.109(S21):M2D1
  #When the PBS has flowed through, cap the bottom and the top of the column until you are ready to add the cell lysate. #*Be sure that the bottom of the column is capped!
  12 KB (1,935 words) - 20:31, 6 April 2021
• 20.109(S21):M2D2
  ... of your concentrated PF3D7_1351100 protein into separate, wells of a flat bottom 96-well plate.
  16 KB (2,534 words) - 11:50, 26 March 2021
• 20.109(S21):M2D6
  ...t a lower temperature than the native folded protein associated with drug (bottom, red lines). The presence of red bands at higher temperatures than blue ba #Retrieve your flasks from the incubator and firmly tap the bottom to dislodge the cells.
  16 KB (2,706 words) - 21:00, 22 April 2021
• 20.109(F21):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  34 KB (5,598 words) - 20:50, 9 September 2021
• 20.109(F21):M2D2
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  21 KB (3,498 words) - 19:42, 15 October 2021
• 20.109(F21):M1D3
  #Use the 'hook' created with the needle to lift the coverslip from the bottom of the well, then use the tweezers to 'catch' the coverslip.
  23 KB (3,807 words) - 20:07, 28 September 2021
• 20.109(F21):M1D4
  #**Lightly touch the tip to the bottom of each imprinted well on the CometChip and ''immediately'' lift the tip fr ... v2.png|center|700px|thumb|'''Microscopy image of loaded CometChip.''' (A) Bottom of macrowell showing evenly spaced microwells. (B) Close-up of empty microw
  15 KB (2,480 words) - 15:38, 23 September 2021
• 20.109(F21):M2D4
  ...f your concentrated PF3D7_20109-F21 protein into separate, wells of a flat bottom 96-well plate.
  15 KB (2,345 words) - 18:33, 21 October 2021
• 20.109(F21):M1D6
  ... were treated with 20 &mu;M H₂O₂. The values at the bottom of the sheet are for the no H₂O₂ samples.
  12 KB (2,055 words) - 19:24, 30 September 2021
• 20.109(S22):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  34 KB (5,639 words) - 21:02, 1 February 2022
• 20.109(S22):M1D3
  #Cap the bottom of the column and then add the slurry to the column. Cap the top of the col #When the PBS has flowed through, recap the bottom of the column. '''Do not dry out the resin! The buffer level should be at t
  13 KB (2,156 words) - 16:27, 14 February 2022
• 20.109(S22):M1D4
  ...;L of your concentrated TDP43-RRM12 protein into separate, wells of a flat bottom 96-well plate.
  12 KB (2,009 words) - 20:13, 15 February 2022
• 20.109(S22):M1D5
  ...repared above, then centrifuge for ~5 seconds to collect the liquid at the bottom of the tubes.
  9 KB (1,413 words) - 14:11, 17 February 2022
• 20.109(S22):M2D1
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  26 KB (4,242 words) - 14:38, 7 March 2022
• 20.109(S22):M2D2
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. *For Fig. 2C, explain the data that are shown (bottom of the panel).
  18 KB (3,019 words) - 16:50, 11 March 2022
• 20.109(F22):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  34 KB (5,645 words) - 20:34, 8 September 2022
• 20.109(F22):M1D1
  #Retrieve your flask from the incubator and firmly tap the bottom to dislodge the cells.
  11 KB (1,744 words) - 14:18, 6 September 2022
• 20.109(F22):M1D4
  #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga #Clean the agarose from the bottom of your CometChip (gelbond side) using a Kimwipe.
  14 KB (2,277 words) - 17:31, 27 September 2022
• 20.109(F22):M1D5
  #Retrieve your flasks from the incubator and firmly tap the bottom to dislodge the cells. ... min then carefully aspirate the media leaving just the cell pellet in the bottom of the conical tube.
  16 KB (2,632 words) - 17:56, 28 September 2022
• 20.109(F22):M1D6
  ...t were treated with 5 &mu;M H₂O₂. The values at the bottom of the sheet are for the no H₂O₂ samples.
  12 KB (1,989 words) - 19:10, 4 October 2022
• 20.109(F22):M2D1
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  21 KB (3,438 words) - 18:03, 13 October 2022
• 20.109(F22):M2D2
  #When the PBS has flowed through, cap the bottom and the top of the column until you are ready to add the cell lysate. #*Be sure that the bottom of the column is capped!
  14 KB (2,346 words) - 19:41, 18 October 2022
• 20.109(F22):M2D3
  ...&mu;L of your concentrated PfFKBP35 protein into separate, wells of a flat bottom 96-well plate.
  14 KB (2,221 words) - 18:10, 20 October 2022
• 20.109(S23):M2D2
  ...ide circle of the plasmid) and the reverse primer is used to replicate the bottom strand (inside circle of the plasmid). The resulting single-stranded produ
  10 KB (1,634 words) - 14:25, 17 March 2023
• 20.109(S23):M2D4
  ... top line of sequence shows the results of the sequencing reaction and the bottom line shows the oligo you designed.
  8 KB (1,291 words) - 16:11, 23 February 2023
• 20.109(S23):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  34 KB (5,644 words) - 21:01, 8 February 2023
• 20.109(S23):M1D3
  ...&mu;L of your concentrated PfFKBP35 protein into separate, wells of a flat bottom 96-well plate.
  14 KB (2,267 words) - 16:32, 5 February 2023
• 20.109(S23):M1D1
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  21 KB (3,363 words) - 18:37, 9 February 2023
• 20.109(S23):M1D4
  #Carefully open the iBlot transfer stack and separate the top stack from the bottom stack (see image to right for iBlot transfer stack setup).[[File:Fa19 iBlot #*Keep the bottom stack in the plastic tray.
  11 KB (1,730 words) - 18:49, 24 February 2023
• 20.109(S23):M2D7
  ...table and scroll down to "Atomic Spectroscopic Information" located at the bottom of the right-hand panel.
  12 KB (1,958 words) - 18:14, 20 April 2023
• 20.109(S23):M2D5
  #Use the 'hook' created with the needle to lift the coverslip from the bottom of the well, then use the tweezers to 'catch' the coverslip.
  10 KB (1,602 words) - 20:14, 11 April 2023
• 20.109(F23):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  34 KB (5,582 words) - 16:04, 6 September 2023
• 20.109(F23):M1D1
  #Retrieve your flask from the incubator and firmly tap the bottom to dislodge the cells.
  11 KB (1,834 words) - 14:25, 18 September 2023
• 20.109(F23):M1D4
  #*Lower the bottom left of the stamp first, then slowly allow the stamp to 'roll' into the aga #Clean the agarose from the bottom of your CometChip (gelbond side) using a Kimwipe.
  14 KB (2,309 words) - 17:56, 26 September 2023
• 20.109(F23):M1D5
  #Retrieve your flasks from the incubator and firmly tap the bottom to dislodge the cells. ... min then carefully aspirate the media leaving just the cell pellet in the bottom of the conical tube.
  18 KB (2,967 words) - 20:18, 28 September 2023
• 20.109(F23):M1D6
  #Select “Browse” from the options in the bottom right of the window. ...t were treated with 5 &mu;M H₂O₂. The values at the bottom of the sheet are for the no H₂O₂ samples.
  9 KB (1,527 words) - 23:32, 2 October 2023
• 20.109(F23):M2D1
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  21 KB (3,438 words) - 17:55, 10 October 2023
• 20.109(F23):M2D2
  #When the PBS has flowed through, cap the bottom and the top of the column until you are ready to add the cell lysate. #*Be sure that the bottom of the column is capped!
  14 KB (2,346 words) - 18:23, 17 October 2023
• 20.109(F23):M2D3
  ...&mu;L of your concentrated PfFKBP35 protein into separate, wells of a flat bottom 96-well plate.
  14 KB (2,227 words) - 15:37, 31 October 2023
• 20.109(S24):Laboratory tour
  ...easure 10, 15 and 20 μL of the 0.1% food coloring stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...asure 20, 50 and 100 μL of the 0.1% food coloring stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
  34 KB (5,582 words) - 20:30, 18 April 2024
• 20.109(S24):M1D1
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for ''EcoRI'' is <font #*Click 'Sequence' from the options at the bottom of the window.
  20 KB (3,337 words) - 18:30, 8 February 2024
• 20.109(S24):M1D3
  ...&mu;L of your concentrated PfFKBP35 protein into separate, wells of a flat bottom 96-well plate.
  14 KB (2,214 words) - 19:31, 15 February 2024
• 20.109(S24):M1D5
  #Retrieve your flask from the incubator and firmly tap the bottom to dislodge the cells.
  12 KB (1,990 words) - 17:03, 28 February 2024
• 20.109(S24):M2D2
  ...ide circle of the plasmid) and the reverse primer is used to replicate the bottom strand (inside circle of the plasmid). The resulting single-stranded produ
  11 KB (1,792 words) - 19:06, 12 March 2024
• 20.109(S24):M2D6
  ...table and scroll down to "Atomic Spectroscopic Information" located at the bottom of the right-hand panel.
  8 KB (1,238 words) - 20:06, 9 April 2024