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  1. 20.109(F16):Complete in silico cloning (Day1)‏‎ (13,384 views)
  2. Resource list: DNA melting and PCR‏‎ (13,368 views)
  3. 20.109(S16):Introduction to cell strains and plating (Day1)‏‎ (13,331 views)
  4. Resource list: Optical trapping‏‎ (13,321 views)
  5. 20.109(F07): M13.1‏‎ (13,265 views)
  6. 20.109(F20):M3D2‏‎ (13,242 views)
  7. Spring 2012:Leanna Morinishi Lit Search‏‎ (13,185 views)
  8. 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)‏‎ (13,133 views)
  9. 20.109(F18):Complete in silico cloning (Day1)‏‎ (13,100 views)
  10. 20.109(S16):Prepare expression system (Day4)‏‎ (13,057 views)
  11. 20.109(S17):Purification of induced protein (Day2)‏‎ (13,037 views)
  12. 20.109(F09):Lab tour‏‎ (12,933 views)
  13. 20.109(S19):In silico cloning and confirmation digest of protein expression vector(Day1)‏‎ (12,888 views)
  14. 20.109(F11):People‏‎ (12,835 views)
  15. Spring 2020 Assignment 7‏‎ (12,792 views)
  16. 20.109(S16):Cell preparation for DNA repair assays (Day5)‏‎ (12,788 views)
  17. 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)‏‎ (12,780 views)
  18. Limits of Detection:Report Requirements‏‎ (12,763 views)
  19. 20.109(F17):Homework‏‎ (12,618 views)
  20. DNA Melter Improvements‏‎ (12,615 views)
  21. ESTORM‏‎ (12,587 views)
  22. 20.109(S09): Transfection (Day5)‏‎ (12,586 views)
  23. 20.109(F10)‏‎ (12,555 views)
  24. Methods‏‎ (12,551 views)
  25. 20.109(S16):Purify protein (Day6)‏‎ (12,543 views)
  26. 20.109(S14)‏‎ (12,449 views)
  27. 20.109(S18):Induce DNA damage and apply drug treatments for cell viability and identification of regulatory motifs in RNA-seq data (Day8)‏‎ (12,447 views)
  28. Spring 11:Directed Evolution‏‎ (12,380 views)
  29. 20.109(S14): Choose system conditions and paper discussion (Day3)‏‎ (12,345 views)
  30. 20.109(F07): Fall 2007 schedule‏‎ (12,344 views)
  31. 20.109(F16):Seed cells for immuno-flourescence assay (Day5)‏‎ (12,260 views)
  32. 20.109(S18):Induce DNA damage and apply drug treatments for RNA purification (Day2)‏‎ (12,233 views)
  33. 20.109(F12):People‏‎ (12,137 views)
  34. Spring 2012: Jonathan Gootenberg Journal‏‎ (12,135 views)
  35. 20.109(S17):In silico cloning and induction of protein expression (Day1)‏‎ (12,131 views)
  36. Shot noise and centroid finding‏‎ (12,111 views)
  37. Electronics boot camp I: passive circuits and transfer functions‏‎ (12,107 views)
  38. 20.109(S20):Characterize clone by titration using flow cytometry (Day4)‏‎ (12,080 views)
  39. Spring 2012:LFM Report‏‎ (12,064 views)
  40. Assignment 9, Part 3: Fitting your data‏‎ (12,027 views)
  41. 20.109(F20):M3D3‏‎ (12,016 views)
  42. Optical Microscopy: Part 2 Report Outline‏‎ (12,003 views)
  43. Spring 11:eSTORM‏‎ (11,971 views)
  44. 20.109(F15):Lipofection (Day6)‏‎ (11,969 views)
  45. Relay Lens Example‏‎ (11,915 views)
  46. 20.109(F23):Fall 2023 schedule‏‎ (11,909 views)
  47. 20.109(S13):People‏‎ (11,895 views)
  48. 20.109(F18):Lab tour‏‎ (11,854 views)
  49. 20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)‏‎ (11,836 views)
  50. 20.109(S18): Assignments‏‎ (11,804 views)
  51. Spring 2012:LFM Proposal‏‎ (11,728 views)
  52. 20.109(F16):Complete immuno-fluorescence assay (Day6)‏‎ (11,683 views)
  53. 20.109(S23):Class data‏‎ (11,656 views)
  54. Spring 2012:Leanna Morinishi Journal‏‎ (11,603 views)
  55. 20.109(S17):Communication‏‎ (11,554 views)
  56. 20.109(S08):Site-directed mutagenesis (Day2)‏‎ (11,480 views)
  57. 20.109(F15):Growth of phage materials (Day1)‏‎ (11,464 views)
  58. 20.109(S14):Lab tour‏‎ (11,252 views)
  59. 20.109(S16)‏‎ (11,221 views)
  60. Lab orientation‏‎ (11,216 views)
  61. Python:Simulating DNA Melting‏‎ (11,205 views)
  62. Optical Microscopy Week 1: Build a brightfield microscope‏‎ (11,157 views)
  63. 20.109(S24):Spring 2024 schedule‏‎ (11,148 views)
  64. Assignment 9, Part 2: Simulating DNA melting data and testing the model function‏‎ (11,141 views)
  65. 20.109(F20):M2D2‏‎ (11,064 views)
  66. 20.109(F18):Growth of phage-based active (cathode) material (Day1)‏‎ (11,036 views)
  67. 20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1)‏‎ (11,022 views)
  68. 20.109(S08):People‏‎ (11,017 views)
  69. 20.109(F08)‏‎ (10,991 views)
  70. 20.109(F16): M1 Data Summary‏‎ (10,964 views)
  71. 20.109(F08):People‏‎ (10,956 views)
  72. 20.109(S08): TA notes for module 1‏‎ (10,926 views)
  73. 20.109(F20):Journal club presentation‏‎ (10,885 views)
  74. 20.109(S20):Class data‏‎ (10,876 views)
  75. 20.109(F16):Design gRNA for CRISPRi (Day2)‏‎ (10,869 views)
  76. 20.109(S23):M2D6‏‎ (10,816 views)
  77. Lab Manual:Introduction to electronics‏‎ (10,811 views)
  78. 20.109(F16):Induce CRISPRi system (Day7)‏‎ (10,775 views)
  79. 20.109(F09): Mod 2 Day 3 Tools for system engineering‏‎ (10,760 views)
  80. 20.109(S20):Journal club presentation (Day4 and 6)‏‎ (10,670 views)
  81. 20.109(F15):Purify protein (Day4)‏‎ (10,669 views)
  82. 20.109(S18): Research article‏‎ (10,644 views)
  83. 20.109(S18):Evaluate protein purity and concentration (Day4)‏‎ (10,480 views)
  84. 20.109(F16):Data analysis (Day7)‏‎ (10,438 views)
  85. 20.109(S16):Complete Western and prepare damaged DNA (Day3)‏‎ (10,436 views)
  86. A.Williams: Objectives and Literature Review‏‎ (10,390 views)
  87. 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)‏‎ (10,362 views)
  88. 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)‏‎ (10,361 views)
  89. 20.109(F17):Complete biochemical experiment (Day5)‏‎ (10,312 views)
  90. 20.109(F17):Develop experiment to optimize loading variables (Day2)‏‎ (10,285 views)
  91. 20.109(S14):DNA repair assays(Day6)‏‎ (10,268 views)
  92. 20.109(S20):Harvest candidate clones (Day2)‏‎ (10,255 views)
  93. 20.109(S19):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5)‏‎ (10,244 views)
  94. 20.109(F20):M1D4‏‎ (10,228 views)
  95. 20.109(S10)‏‎ (10,211 views)
  96. 20.109(F09):People‏‎ (10,150 views)
  97. 20.109(F07): Module 2 oral presentations‏‎ (10,131 views)
  98. 20.109(F18):Communication‏‎ (10,125 views)
  99. 20.109(F20):M2D5‏‎ (10,093 views)
  100. 20.109(S08): Spring 2008 schedule‏‎ (10,076 views)
  101. 20.109(S14):Microbiome summary‏‎ (9,962 views)
  102. 20.109(S07):Bio-material engineering/Screening library‏‎ (9,925 views)
  103. 20.109:Module 4‏‎ (9,912 views)
  104. 20.109(S14):People‏‎ (9,912 views)
  105. Resource list: Suppliers‏‎ (9,908 views)
  106. 20.109(S23):M2D1‏‎ (9,882 views)
  107. 20.109(F19):Load cells into CometChip and apply treatments (Day4)‏‎ (9,871 views)
  108. 20.109(S16): Protein engineering summary‏‎ (9,866 views)
  109. 20.109(F20):M3D1‏‎ (9,802 views)
  110. Beam Expander Example‏‎ (9,786 views)
  111. Spring 2012:Leanna Morinishi Lab 2‏‎ (9,784 views)
  112. 20.109(S07): Start-up signal measurement‏‎ (9,758 views)
  113. Spring 2012:PSF lab‏‎ (9,725 views)
  114. 20.109(F15):Battery assembly and testing (Day5)‏‎ (9,684 views)
  115. 20.109(S16):DNA repair assays (Day6)‏‎ (9,649 views)
  116. 20.109(F19):Prepare for cellular thermal shift assay (Day1)‏‎ (9,646 views)
  117. 20.109(S21):Module 3‏‎ (9,646 views)
  118. 20.109(F17):Prepare comet chips (Day1)‏‎ (9,641 views)
  119. 20.109(F18):Measure fermentation products (Day8)‏‎ (9,583 views)
  120. 20.109(S16):Flow cytometry and paper discussion (Day7)‏‎ (9,541 views)
  121. 20.109(S20):Complete in silico cloning and induce TDP43 protein expression (Day1)‏‎ (9,508 views)
  122. 20.109(S22):M1D2‏‎ (9,481 views)
  123. 20.109(F20):M1D7‏‎ (9,468 views)
  124. 20.109(F09): Journal Club I‏‎ (9,451 views)
  125. Spring 2012:Vincent Lee Journal‏‎ (9,403 views)
  126. 20.109(F20):M2D6‏‎ (9,396 views)
  127. 20.109(F19):Communication‏‎ (9,375 views)
  128. 20.109(S08):Student presentations (M2D8)‏‎ (9,351 views)
  129. Optical Microscopy: Part 3 Report Outline‏‎ (9,337 views)
  130. 20.109(S18):Laboratory tour‏‎ (9,321 views)
  131. 20.109(F19):Image CometChip (Day6)‏‎ (9,309 views)
  132. 20.109(F16):Generate gRNA plasmid (Day3)‏‎ (9,304 views)
  133. 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)‏‎ (9,295 views)
  134. 20.109(F09): Journal Club II‏‎ (9,284 views)
  135. 20.109(S21):M1D5‏‎ (9,248 views)
  136. 20.109(S18):Analyze small microarray data (Day3)‏‎ (9,229 views)
  137. 20.109(F08): Fall 2008 schedule‏‎ (9,226 views)
  138. 20.109(S13):Design mutant (Day1)‏‎ (9,207 views)
  139. 20.109(F10): Fall 2010 schedule‏‎ (9,202 views)
  140. 20.109(S17):Evaluation of purified protein (Day3)‏‎ (9,189 views)
  141. 20.109(S16):Module 1‏‎ (9,153 views)
  142. Cellular microrheology‏‎ (9,149 views)
  143. 20.109(S23):Journal article presentation‏‎ (9,123 views)
  144. 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)‏‎ (9,031 views)
  145. 20.109(S14):Data analysis (Day7)‏‎ (8,980 views)
  146. Recording, displaying and saving images in MATLAB‏‎ (8,964 views)
  147. 20.109(S21):Communication‏‎ (8,958 views)
  148. Results‏‎ (8,958 views)
  149. 20.109(S17):Screen chemical library for FKBP12 binders (Day4)‏‎ (8,949 views)
  150. 20.109(S22):M1D3‏‎ (8,918 views)
  151. 20.109(F20):M3D5‏‎ (8,909 views)
  152. 20.109(S17):Examine quantitative PCR results (Day9)‏‎ (8,900 views)
  153. DNA Melting II: Using the Matlab DNALockIn GUI‏‎ (8,892 views)
  154. 20.109(S13):Context-setting and primer design (Day1)‏‎ (8,884 views)
  155. Resource list: Electronics‏‎ (8,879 views)
  156. 20.109(F20): Assignments‏‎ (8,851 views)
  157. 20.109(F20):Communication‏‎ (8,834 views)
  158. 20.109(F07)‏‎ (8,832 views)
  159. 20.109(F20):M1D3‏‎ (8,821 views)
  160. 20.109(F17):Communication‏‎ (8,817 views)
  161. Compound Microscope Example‏‎ (8,806 views)
  162. Williams: PSF and Deconvolution Lab 2‏‎ (8,802 views)
  163. 20.109(F07):Module 1‏‎ (8,774 views)
  164. 20.109(F12): Journal Club II‏‎ (8,774 views)
  165. 20.109(F17):Evaluate cell loading results (Day3)‏‎ (8,754 views)
  166. 20.109(S18):Investigate RNA-seq data using public databases (Day5)‏‎ (8,740 views)
  167. 20.109(F20):Module 2‏‎ (8,736 views)
  168. 20.109(S22):Homework‏‎ (8,701 views)
  169. 20.109(S19):Laboratory tour‏‎ (8,657 views)
  170. 20.109(S21):M2D2‏‎ (8,652 views)
  171. 20.109(F16): Research article‏‎ (8,651 views)
  172. 20.109(S07): Western analysis‏‎ (8,647 views)
  173. 20.109(F17):Test role of biochemical factors in genomic stability (Day4)‏‎ (8,628 views)
  174. 20.109(S08): TA notes for module 2‏‎ (8,626 views)
  175. 20.109(F15):Cathode construction (Day3)‏‎ (8,592 views)
  176. Spring 2011:CellFinder‏‎ (8,592 views)
  177. 20.109(S08):Module 2‏‎ (8,580 views)
  178. 20.109(F22):Homework‏‎ (8,578 views)
  179. 20.109(F08): Sign up for journal club‏‎ (8,548 views)
  180. 20.109(S20):Communication‏‎ (8,528 views)
  181. 20.109(F17):Complete in silico cloning (Day1)‏‎ (8,512 views)
  182. 20.109(F19):Begin Western blot analysis (Day3)‏‎ (8,506 views)
  183. 20.109(F20):M1D1‏‎ (8,495 views)
  184. 20.109(S09): siRNA design (Day1)‏‎ (8,454 views)
  185. 20.109(F19):Complete in silico cloning of pdCas9 (Day1)‏‎ (8,452 views)
  186. 20.109(F18):Practice tissue culture and prepare microwell array (Day1)‏‎ (8,452 views)
  187. 20.109(S16): System engineering report‏‎ (8,408 views)
  188. 20.109(F07): ECD experimental variations‏‎ (8,406 views)
  189. DNA Melting: DNA Sequences‏‎ (8,389 views)
  190. 20.109(F22):M2D7‏‎ (8,372 views)
  191. 20.109(F07):Module 2‏‎ (8,311 views)
  192. 20.109(S18): Prep notes for M1‏‎ (8,311 views)
  193. Spring 2012 Bioinstrumentation Project Lab‏‎ (8,301 views)
  194. Lab 1 Report -- Nathan S Lachenmyer‏‎ (8,244 views)
  195. MRI lab: FPGA controller documentation‏‎ (8,207 views)
  196. 20.109(S13):PCR and paper discussion (Day3)‏‎ (8,134 views)
  197. 20.109(S16):Site-directed mutagenesis (Day3)‏‎ (8,107 views)
  198. 20.109(S14):Complete Western and prepare damaged DNA (Day4)‏‎ (8,085 views)
  199. 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)‏‎ (8,039 views)
  200. 20.109(S11)‏‎ (8,039 views)
  201. 20.109(S20):Analyze RNA-seq data and select gene targets for quantitative PCR (qPCR) experiment (Day 6)‏‎ (8,023 views)
  202. 20.109(S21):Data Summary‏‎ (8,022 views)
  203. Bryan Hernandez/20.109/Lab notebook/Module 1/Day 4‏‎ (8,019 views)
  204. 20.109(F15):People‏‎ (8,017 views)
  205. 20.109(S20):Assess purity and concentration of TDP43 protein (Day3)‏‎ (8,007 views)
  206. 20.109(S08):Testing cell viability (Day3)‏‎ (7,977 views)
  207. 20.109(S22):Journal club presentation‏‎ (7,975 views)
  208. 20.109(S13):DNA cloning (Day4)‏‎ (7,948 views)
  209. 20.109(S13)‏‎ (7,946 views)
  210. 20.109(F17): Assignments‏‎ (7,927 views)
  211. Procedure: Particle tracking‏‎ (7,926 views)
  212. 20.109(S18):Purify RNA and practice RNA-seq data analysis methods (Day3)‏‎ (7,872 views)
  213. 20.109(S08):Induce protein expression (Day4)‏‎ (7,835 views)
  214. 20.109(S19):Communication‏‎ (7,818 views)
  215. 20.109(F16):Measure DNA damage with comet chip (Day3)‏‎ (7,816 views)
  216. 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)‏‎ (7,802 views)
  217. 20.109(F19):Complete CometChip experiment (Day5)‏‎ (7,801 views)
  218. Optical Microscopy Week 3: Experiments‏‎ (7,800 views)
  219. 20.109(F16): Announcements‏‎ (7,793 views)
  220. 20.109(F15):Wiki basics‏‎ (7,777 views)
  221. 20.109(F11): Fall 2011 schedule‏‎ (7,756 views)
  222. Resource list: Magnetic Resonance Imaging‏‎ (7,745 views)
  223. 20.109(F12): Assignments‏‎ (7,716 views)
  224. 20.109(S07): Lipofection‏‎ (7,682 views)
  225. 20.109(S10): Spring 2010 schedule‏‎ (7,628 views)
  226. Assignment 8, Part 1: convolution‏‎ (7,621 views)
  227. 20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7)‏‎ (7,591 views)
  228. 20.109(S18):Growth of phage-based active (cathode) material (Day1)‏‎ (7,577 views)
  229. 20.109(S17):Prepare Western blot and induce DNA damage for quantitative PCR assay (Day2)‏‎ (7,565 views)
  230. 20.109(S14):Introduction to cell strains and plating (Day1)‏‎ (7,527 views)
  231. 20.109(F12): Journal Club I‏‎ (7,504 views)
  232. 20.109(F15):Assess protein function (Day7)‏‎ (7,501 views)
  233. 20.109(S07): cDNA synthesis and microarray‏‎ (7,462 views)
  234. 20.109(F08):Lab tour‏‎ (7,456 views)
  235. 20.109(S19):Complete data analysis (Day9)‏‎ (7,444 views)
  236. 20.109(S16):People‏‎ (7,428 views)
  237. 20.109(S08)‏‎ (7,422 views)
  238. 20.109(S09):Prepare expression system (Day4)‏‎ (7,421 views)
  239. 20.109(S18):Purify protein for secondary assays(Day3)‏‎ (7,415 views)
  240. 20.109(F09): Mod 2 Day 5 Assessing re-tuned system‏‎ (7,408 views)
  241. 20.109(S21):M2D5‏‎ (7,391 views)
  242. 20.109(S17):Complete cell viability assay (Day9)‏‎ (7,384 views)
  243. 20.109(F09): Mod 2 Day 1 Testing an engineered biological system‏‎ (7,339 views)
  244. DNA Melting: Using the Matlab DNAMelter GUI‏‎ (7,325 views)
  245. 20.109(S14):Begin Western protein analysis (Day2)‏‎ (7,287 views)
  246. Final Project -- Nathan S Lachenmyer‏‎ (7,285 views)
  247. 20.109(S14):Preparing cells for analysis (Day4)‏‎ (7,263 views)
  248. 20.109(S21):M2D1‏‎ (7,242 views)
  249. 20.109(F19):Induce CRISPRi system (Day7)‏‎ (7,225 views)
  250. 20.109(S14): TA notes for module 1‏‎ (7,211 views)

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