Final Project -- Nathan S Lachenmyer

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Confocal Microscopy

Literature

  • Imaging in Neuroscience and Development: A Laboratory Manual / editors, Rafael Yuste and Arthur Konnerth
  • Confocal microscopy for biologists / Alan R. Hibbs.
  • Handbook of biological confocal microscopy / editor, James B. Pawley.

Goals and Checkpoints

Goals

Key Factors Proposed Technical Targets Requirements for Success Current Practice Associated Technical Barriers
OCLSM Cost < $8,000 < $15,000 > $100,000 commercial; ~$30,000 DIY Cheap replacement for PMT & Galvo, etc.
OCLSM Scan Time 1 frame/second 1 frame / 10 seconds 10-50 fps Fast scanning mirrors + ADC time

Timeline

  • 2 Weeks: Brightfield Confocal Microscopy
  • 2 Weeks: Software Image Capture, PSF Characterized, Z-Stack Images
  • 3 Weeks: Laser Scanning Features

Proposed Design

Challenges

  • Control software
  • Getting good resolution

Weekly Updates

2012 April 8

  • Look for PMTs and/or Galvos

2012 April 15

  • Calculations for stuff

2012 April 22

  • Construct Galvos, Program Maple

2012 April 29

  • Write-up for Fluorescence Microscope

2012 May 06