20.109:Module 4
Module 4
TA: [3]
In this experimental module we will study an unusual protein, one that allows yeast to bind a metal, gold. Over the next few weeks we will purify yeast based on this binding property, and then we���ll vary some experimental condition to improve the yeast/gold interaction. Using your optimized conditions, we will screen a library of yeast to isolate a new gold-binding strain. The DNA encoding the new gold-binding protein will be sequenced and, with any luck, we���ll elucidate some amino acid requirements for the yeast/metal interaction.
Module 4 Day 1: Screening library
Module 4 Day 2: Optimizing panning
Module 4 Day 3: Rescreening gold binders
Module 4 Day 4: PCR of gold binding candidates
| Module 4 Day 5: Student presentations
Module 4 Day 6: Sequence analysis
