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  1. Lab Manual: Atomic Force Microscopy (AFM)‏‎ (421,923 views)
  2. 20.309 Main Page‏‎ (321,089 views)
  3. DNA Melting Part 1: Measuring Temperature and Fluorescence‏‎ (291,683 views)
  4. Main Page‏‎ (288,701 views)
  5. Electronics primer‏‎ (245,186 views)
  6. Electronics Mini-Lab‏‎ (239,413 views)
  7. Geometrical optics and ray tracing‏‎ (204,806 views)
  8. Lab Manual: Optical Microscopy‏‎ (148,133 views)
  9. Assignment 2 Part 1: Noise in images‏‎ (144,283 views)
  10. 20.345:Course Information‏‎ (142,072 views)
  11. Optics Bootcamp‏‎ (124,088 views)
  12. 20.309:Course Information‏‎ (119,485 views)
  13. DNA Melting: Simulating DNA Melting - Intermediate Topics‏‎ (114,509 views)
  14. DNA Melting Part 2: Lock-in Amplifier and Temperature Control‏‎ (109,858 views)
  15. Error analysis‏‎ (109,493 views)
  16. Assignment 4 part 2: Measure resolution‏‎ (106,928 views)
  17. Assignment 1, Part 3: Building your transillumination microscope‏‎ (105,775 views)
  18. Optical Microscopy Part 1: Brightfield Microscopy‏‎ (104,026 views)
  19. Assignment 2: fluorescence microscopy‏‎ (96,593 views)
  20. Optical detectors, noise, and the limit of detection‏‎ (89,638 views)
  21. Impedance Analysis‏‎ (88,514 views)
  22. Assignment 1, Part 2: Optics bootcamp‏‎ (88,321 views)
  23. 20.109 Main Page‏‎ (86,025 views)
  24. Assignment 3, Part 1: visualizing actin with fluorescence contrast‏‎ (84,111 views)
  25. Bode plots‏‎ (83,573 views)
  26. Limits of Detection: Data Sessions‏‎ (83,316 views)
  27. Lab Manual: Limits of Detection‏‎ (83,084 views)
  28. Estimating second order system parameters from noise power spectra using nonlinear regression‏‎ (83,055 views)
  29. DNA Melting Report Requirements‏‎ (82,193 views)
  30. Assignment 1 Overview: Transillumination microscopy‏‎ (81,604 views)
  31. Understanding log plots‏‎ (78,090 views)
  32. DNA Melting: Model function and parameter estimation by nonlinear regression‏‎ (77,400 views)
  33. Todo List‏‎ (76,692 views)
  34. Assignment 4 part 1: Make a fake image‏‎ (76,282 views)
  35. Electronics written problems‏‎ (74,895 views)
  36. Assignment 1, Part 4: Measuring magnification and bead size‏‎ (73,514 views)
  37. DNA Melting: Simulating DNA Melting - Basics‏‎ (71,459 views)
  38. Optical Microscopy Part 3: Resolution and Stability‏‎ (71,307 views)
  39. Spring 2020 Assignment 9‏‎ (71,286 views)
  40. 20.109(F18): Fall 2018 schedule‏‎ (70,412 views)
  41. Exam 2 study guide‏‎ (67,717 views)
  42. Assignment 10, Part 1: Measuring the osmotic shock response of yeast‏‎ (65,127 views)
  43. Physical optics and resolution‏‎ (65,085 views)
  44. Assignment 8, Part 3: add flow control and test your device‏‎ (63,392 views)
  45. Lab Manual: Measuring DNA Melting Curves‏‎ (62,602 views)
  46. Optical Microscopy Part 2: Fluorescence Microscopy‏‎ (62,346 views)
  47. 20.109(S19): Spring 2019 schedule‏‎ (60,573 views)
  48. Assignment 2 Part 2: Fluorescence microscopy‏‎ (59,668 views)
  49. Protocols for cell culture‏‎ (58,708 views)
  50. Optical Microscopy Part 4: Particle Tracking‏‎ (58,252 views)
  51. 20.109(S17): Spring 2017 schedule‏‎ (57,577 views)
  52. Assignment 2 Part 3: Build an epi-illuminator for your microscope‏‎ (56,966 views)
  53. 20.109(F16): Fall 2016 schedule‏‎ (54,175 views)
  54. Microscopy report outline‏‎ (53,935 views)
  55. Electronics boot camp lab part 1‏‎ (53,647 views)
  56. 20.109(S20): Spring 2020 schedule‏‎ (53,216 views)
  57. 20.109(S18): Spring 2018 schedule‏‎ (52,469 views)
  58. Cell Reservations‏‎ (52,269 views)
  59. Assignment 1, Part 1: Pre-lab questions‏‎ (51,058 views)
  60. 20.109(F19):Class data‏‎ (50,066 views)
  61. Input and output impedance‏‎ (49,967 views)
  62. 20.109(S19):Class data‏‎ (49,856 views)
  63. 20.109(F20): Fall 2020 schedule‏‎ (49,540 views)
  64. 20.109(S18):Class data‏‎ (48,943 views)
  65. Manta G032 camera measurements‏‎ (48,349 views)
  66. Assignment 9, Part 1: Analyze two-color yeast images‏‎ (48,145 views)
  67. MATLAB: Estimating resolution from a PSF slide image‏‎ (47,911 views)
  68. DNA Melting Thermodynamics‏‎ (47,020 views)
  69. 20.109(S21):Spring 2021 schedule‏‎ (46,694 views)
  70. Complex Number Review‏‎ (45,661 views)
  71. Assignment 4 part 3: Track microspheres over time‏‎ (45,509 views)
  72. 20.109(F18):Class data‏‎ (45,335 views)
  73. 20.109(F17): Fall 2017 Schedule‏‎ (42,915 views)
  74. Assignment 8, Part 2: fabricate a microfluidic device‏‎ (42,019 views)
  75. 20.109(S21):M1D1‏‎ (40,944 views)
  76. 20.109(F16):Communication‏‎ (39,666 views)
  77. 20.109(F19): Fall 2019 schedule‏‎ (39,129 views)
  78. 20.109(F15):Homework‏‎ (37,905 views)
  79. DNA Melting Report Requirements for Part 1‏‎ (37,695 views)
  80. 20.309:Lab Report Guidelines‏‎ (36,035 views)
  81. 20.109(S11):Journal club (Day8)‏‎ (35,877 views)
  82. 20.109(S16):Homework‏‎ (35,335 views)
  83. Spring 2012:Leanna Morinishi Lab 1‏‎ (35,078 views)
  84. Assignment 8, Part 0: convolution practice‏‎ (35,077 views)
  85. Flat-field correction‏‎ (34,713 views)
  86. DNA melting: Identifying the unknown sample‏‎ (34,694 views)
  87. Converting Gaussian fit to Rayleigh resolution‏‎ (34,635 views)
  88. 20.109(F18):Homework‏‎ (34,280 views)
  89. 20.109(F16):Test comet chip loading variables (Day2)‏‎ (34,031 views)
  90. Fall 2012: Journal Presentations‏‎ (33,776 views)
  91. 20.109(S16):In situ cloning (Day1)‏‎ (33,594 views)
  92. ThorLabs OTKB optical trapping kit‏‎ (33,563 views)
  93. Assignment 8, Part 2: lock-in amplifier and temperature control‏‎ (32,762 views)
  94. Laser cutter safe operating procedure‏‎ (32,317 views)
  95. 20.109(S18):Journal club presentations (Day6 and 7)‏‎ (32,288 views)
  96. 20.109‏‎ (32,039 views)
  97. Optical Microscopy: Part 4 Report Outline‏‎ (31,218 views)
  98. 20.109(S16): Spring 2016 schedule‏‎ (31,211 views)
  99. Assignment 5 Overview‏‎ (30,266 views)
  100. 20.109(S21):M1D2‏‎ (29,889 views)
  101. 20.109(S20):Journal club presentations (Day4 and 5)‏‎ (29,338 views)
  102. 20.109(S19):Journal club presentations (Day7 and 8)‏‎ (29,315 views)
  103. Real electronics‏‎ (29,141 views)
  104. Assignment 7, Part 2: measure temperature and fluorescence‏‎ (27,317 views)
  105. 20.109(F16):Homework‏‎ (27,298 views)
  106. 20.109(S09): Module 2 oral presentations‏‎ (27,097 views)
  107. 20.109:People‏‎ (26,998 views)
  108. Assignment 3 Overview‏‎ (26,816 views)
  109. Spring 11:QRT-PCR‏‎ (26,605 views)
  110. 20.109(S21):M3D3‏‎ (26,580 views)
  111. 20.109(S20):Homework‏‎ (26,444 views)
  112. Calculating MSD and Diffusion Coefficients‏‎ (26,126 views)
  113. 20.109(S08):Data analysis (Day7)‏‎ (25,930 views)
  114. 20.109(F16):Lab tour‏‎ (25,688 views)
  115. 20.109(S21):Homework‏‎ (25,294 views)
  116. 20.109(F12)‏‎ (25,034 views)
  117. 20.109(S17):Homework‏‎ (24,867 views)
  118. 20.109(S13):Journal club II (Day8)‏‎ (24,744 views)
  119. 20.109(F15): Fall 2015 schedule‏‎ (24,613 views)
  120. 20.109(S16):Begin Western blot protein analysis and choose system conditions (Day2)‏‎ (24,565 views)
  121. 20.109(F21):Homework‏‎ (24,136 views)
  122. Problem Set 1‏‎ (24,047 views)
  123. Electronics bootcamp II: feedback systems‏‎ (23,317 views)
  124. 20.109(S21):M1D7‏‎ (23,118 views)
  125. 20.109(S18):Homework‏‎ (22,853 views)
  126. Aperture and field stops‏‎ (22,778 views)
  127. 20.109(S21):M2D6‏‎ (22,700 views)
  128. Optical aberrations‏‎ (22,488 views)
  129. 20.109(S14):Journal club II (Day8)‏‎ (22,397 views)
  130. 20.109(F17):Journal club II (Day 6)‏‎ (22,380 views)
  131. Spring 2011:Optical Trapping Lab‏‎ (22,313 views)
  132. Spring 2011:3D PSF lab‏‎ (22,304 views)
  133. 20.109(S16):Journal Club II (Day8)‏‎ (22,279 views)
  134. 20.109(S07):Start-up genome engineering‏‎ (21,872 views)
  135. 20.109(F21):Fall 2021 schedule‏‎ (21,762 views)
  136. 20.109(F20):Lab tour‏‎ (21,632 views)
  137. 20.109(S22):Class data‏‎ (21,516 views)
  138. 20.109(F20):M3D4‏‎ (21,183 views)
  139. Assignment 10 Overview‏‎ (21,065 views)
  140. 20.109(S17):Journal Club II (Day8)‏‎ (21,012 views)
  141. Understanding the lock-in amplifier‏‎ (20,930 views)
  142. Optical microscopy lab wiki pages‏‎ (20,743 views)
  143. 20.109(S22):Spring 2022 schedule‏‎ (20,729 views)
  144. 20.109(F16):Journal club II (Day 6)‏‎ (20,674 views)
  145. Sandbox‏‎ (20,468 views)
  146. Spring 2020 Assignment 8‏‎ (20,371 views)
  147. DNA melting lab wiki pages‏‎ (20,360 views)
  148. 20.109(S21):M1D4‏‎ (20,283 views)
  149. Assignment 5, Part 1: MSD difference tracking and microscope stability‏‎ (20,130 views)
  150. 20.109(F20):M1D2‏‎ (19,931 views)
  151. Assignment 4: finding and measuring things‏‎ (19,800 views)
  152. Spring 2012: Ajoke Jumoke Williams‏‎ (19,746 views)
  153. 20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)‏‎ (19,656 views)
  154. Confocal Wiki‏‎ (19,588 views)
  155. Spring 2012:LFM Documentation‏‎ (19,525 views)
  156. 20.109(F07):People‏‎ (19,520 views)
  157. Protocols‏‎ (19,260 views)
  158. Optical trap‏‎ (19,066 views)
  159. Williams: Optical Trapping Lab 1‏‎ (19,021 views)
  160. 20.109(S21):M3D2‏‎ (19,010 views)
  161. 20.109(F18):Journal club presentation (Day4 and 6)‏‎ (18,832 views)
  162. 20.109(F19):Journal club presentation (Day4 and 6)‏‎ (18,522 views)
  163. 20.109(S10):People‏‎ (18,436 views)
  164. 20.109(S07): Measuring calcium in vitro‏‎ (18,417 views)
  165. 20.109(F15):Journal Club II (Day8)‏‎ (18,388 views)
  166. Photobleaching Model‏‎ (18,284 views)
  167. Assignment 5, Part 2: live cell particle tracking of endocytosed beads‏‎ (18,280 views)
  168. 20.109(F11)‏‎ (18,204 views)
  169. 20.109(F20):M2D1‏‎ (18,193 views)
  170. 20.109(F12) Pre-Proposal: Engineering Viral Magnetic Nanoparticles for Magnetic Hyperthermic Cancer Therapy‏‎ (18,110 views)
  171. 20.109(S21):Journal club presentation‏‎ (18,110 views)
  172. 20.109(S07): Student presentations, module 2‏‎ (18,011 views)
  173. In silico cloning of pdCas9 construct‏‎ (18,011 views)
  174. Assignment 3, Part 2: experimental design with fluorescence‏‎ (17,814 views)
  175. 20.109(S21):M3D1‏‎ (17,801 views)
  176. Assignment 8 Overview: flow channel & two-color microscope‏‎ (17,691 views)
  177. 20.109: Spring 2007 schedule‏‎ (17,489 views)
  178. Assignment 8 Overview‏‎ (17,480 views)
  179. 20.109(S21):M1D6‏‎ (17,453 views)
  180. 20.109(S10):Journal club (Day8)‏‎ (17,409 views)
  181. 20.109(S21):M1D3‏‎ (17,379 views)
  182. 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)‏‎ (17,366 views)
  183. Assignment 9 Overview‏‎ (17,361 views)
  184. 20.109(S19):Purify protein for secondary assays(Day2)‏‎ (17,312 views)
  185. 20.345:Main page‏‎ (17,226 views)
  186. Assignment 7 Overview‏‎ (17,018 views)
  187. Two-color Yeast Protocols‏‎ (16,985 views)
  188. 20.109(F20):M1D5‏‎ (16,849 views)
  189. Assignment 6, Part 2: Electronics bootcamp‏‎ (16,654 views)
  190. 20.109(F12): Fall 2012 schedule‏‎ (16,539 views)
  191. 20.109(S13): Spring 2013 schedule‏‎ (16,399 views)
  192. 20.109(F19):Homework‏‎ (16,288 views)
  193. Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking‏‎ (16,192 views)
  194. 20.345:Final project proposal‏‎ (16,179 views)
  195. Matlab:Image Processing for Fluorescent Microscopy‏‎ (16,162 views)
  196. 20.109(S21):M3D4‏‎ (16,153 views)
  197. Assignment 8, Part 2: build a two-color microscope‏‎ (15,994 views)
  198. Assignment 9, Part 1: model function‏‎ (15,982 views)
  199. 20.109(F19):Treat cells for gamma-H2AX (Day2)‏‎ (15,975 views)
  200. 20.309:Safety‏‎ (15,902 views)
  201. 20.109(S07): Start-up expression engineering‏‎ (15,788 views)
  202. Temporary Optics Bootcamp‏‎ (15,749 views)
  203. 20.109(S14): Spring 2014 schedule‏‎ (15,710 views)
  204. 20.109(S23):Spring 2023 schedule‏‎ (15,705 views)
  205. Matlab: Scalebars‏‎ (15,675 views)
  206. 20.109(S17):Complete cell survival assay and examine transcript levels in response to DNA damage (Day 4)‏‎ (15,626 views)
  207. 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)‏‎ (15,564 views)
  208. Spring 11:Chemotaxis Assay‏‎ (15,528 views)
  209. 20.109(S17):Purify RNA for quantitative PCR assay and treat cells for survival assay (Day3)‏‎ (15,363 views)
  210. 20.109(F16):Module 1‏‎ (15,339 views)
  211. 20.109(F15)‏‎ (15,331 views)
  212. Assignment 5, Part 1: viscosity and diffusivity in glycerol-water mixtures‏‎ (15,248 views)
  213. 20.109(F20):M2D3‏‎ (15,165 views)
  214. 20.109(S09):People‏‎ (15,067 views)
  215. 20.109(S18):Analyze small microarray data and induce protein expression (Day2)‏‎ (15,032 views)
  216. 20.109(S20):Identify clones to characterize (Day3)‏‎ (15,018 views)
  217. 20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)‏‎ (14,812 views)
  218. 20.109(F08): Mod 2 Day 1 Protein engineering with PCR‏‎ (14,802 views)
  219. 20.109(F16):Prepare comet chips (Day1)‏‎ (14,778 views)
  220. 20.109(S19):Evaluate protein purity and concentration (Day4)‏‎ (14,736 views)
  221. 20.109(S19):Homework‏‎ (14,630 views)
  222. 20.109(S07): Agarose gel electrophoresis‏‎ (14,530 views)
  223. Assignment 7, Part 1: op amp golden rules questions‏‎ (14,479 views)
  224. 20.109(F15):Prepare expression system (Day2)‏‎ (14,383 views)
  225. 20.109(S21):Module 1‏‎ (14,324 views)
  226. 20.109(F16): Assignments‏‎ (14,269 views)
  227. 20.109(F20):M1D6‏‎ (13,958 views)
  228. 20.109(F22):Fall 2022 schedule‏‎ (13,958 views)
  229. 20.109(S11):People‏‎ (13,913 views)
  230. 20.109(S16):Design mutation primers (Day2)‏‎ (13,853 views)
  231. 20.109(S20):Purify TDP43 protein (Day2)‏‎ (13,837 views)
  232. Procedure: Diffusion in biological gels‏‎ (13,808 views)
  233. Assignment 11: Tell us about your lab visit‏‎ (13,797 views)
  234. Assignment 6 Overview‏‎ (13,762 views)
  235. 20.109(F15):Induce protein and evaluate DNA (Day3)‏‎ (13,728 views)
  236. 20.109(S17):Complete data analysis (Day6)‏‎ (13,728 views)
  237. 20.109(F16):Confirm gRNA sequence (Day5)‏‎ (13,723 views)
  238. Spring 2012:Leanna Morinishi‏‎ (13,672 views)
  239. Using tfest to find a system model‏‎ (13,588 views)
  240. 20.109(S20):Analyze titration curves (Day5)‏‎ (13,581 views)
  241. 20.109(F09): Fall 2009 schedule‏‎ (13,572 views)
  242. 20.109(F20):Homework‏‎ (13,563 views)
  243. 20.109(F09)‏‎ (13,533 views)
  244. 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)‏‎ (13,483 views)
  245. Spring 2020 Assignment 6‏‎ (13,473 views)
  246. Theory‏‎ (13,392 views)
  247. Assignment 9 Overview: Analyzing yeast images‏‎ (13,385 views)
  248. BE Classroom Computer Documentation‏‎ (13,377 views)
  249. 20.109(F16):Complete in silico cloning (Day1)‏‎ (13,363 views)
  250. 20.109(F18): Assignments‏‎ (13,353 views)

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