20.109(F17): Fall 2017 Schedule

From Course Wiki
Jump to: navigation, search
20.109(F17): Laboratory Fundamentals of Biological Engineering

Fa17 banner image.png

Fa17 Schedule        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11-12 (16-220)
Laboratory section times: T/R 1-5 or W/F 1-5 (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sept 7/8 NLL
Orientation lecture
Orientation
Prelab T/R
Prelab W/F
1 1 T/W Sept 12/13 BE Prepare microwell array and practice tissue culture
Prelab T/R
Prelab W/F
Laboratory orientation quiz
Homework due
1 2 R/F Sept 14/15 NLL Develop experiment to optimize cell loading
Prelab T/R
Prelab W/F
Homework due
1 3 T/W Sept 19/20 BE Evaluate cell loading results
Prelab T/R
Prelab W/F
Homework due
1 4 R/F Sept 21/22 BE Test role of biochemical factors in genomic stability
Prelab T/R
Prelab W/F
Homework due
1 5 T/W Sept 26/27 BE Complete biochemical experiment and apply chemical treatments for sub-nuclear foci assay
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
R/F Sept 28/29 BE Lecture, but no laboratory
Career fair student holiday
1 6 T/W Oct 3/4 BE Examine sub-nuclear foci abundance to measure DNA damage
Prelab T/R
Prelab W/F
Homework due
1 7 R/F Oct 5/6 BE Visualize and analyze data for sub-nuclear foci assay
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
T/W Oct 10/11 Columbus day holiday Data Summary draft due Wed, Oct 11 at 10 pm
2 1 R/F Oct 12/13 NLL
Lecture M2D1
Complete in silico cloning
Prelab T/R
Prelab W/F
Homework due
Mini-presentation due Sat, Oct 14 at 10 pm
2 2 T/W Oct 17/18 NLL
Lecture M2D2
Design gRNA for CRISPRi
Prelab T/R
Prelab W/F
Homework due
2 3 R/F Oct 19/20 NLL
Generate gRNA plasmid
Prelab T/R
Prelab W/F
Homework due
Data Summary revision due Sun, Oct 22 at 10 pm
2 4 T/W Oct 24/25 NLL
Lecture M2D4
Journal club I Blog post due Mon, Oct 23 at 10 pm
2 5 R/F Oct 26/27 NLL
Lecture M2D5
Confirm gRNA sequence
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
2 6 T/W Oct/Nov 31/1 NLL
Lecture M2D6
Journal club II
2 7 R/F Nov 2/3 NLL
Lecture M2D7
Induce CRISPRi system
Prelab T/R
Prelab W/F
Homework due
2 8 T/W Nov 7/8 NLL
Lecture M2D8
Measure fermentation products
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
R/F Nov 9/10 Veterans' day holiday
3 1 T/W Nov 14/15 AB Prepare phage materials
Prelab T/R
Prelab W/F
Homework due
3 2 R/F Nov 16/17 AB Purify active material for cathode construction
Prelab T/R
Prelab W/F
Homework due
T/W Nov 21/22 AB
Lecture
Lecture, but no laboratory Research article due Mon, Nov 20 at 10 pm
Blog post due Tue, Nov 21 at 10 pm
R/F Nov 23/24 Thanksgiving holiday
3 3 T/W Nov 28/29 AB Construct cathode with active material
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
3 4 R/F Nov/Dec 30/1 AB
Lecture
Visualize active material using TEM
Prelab T/R
Prelab W/F
Homework due
3 5 T/W Dec 5/6 AB
Lecture
Assemble and test batteries
Prelab T/R
Prelab W/F
Laboratory quiz
Homework due
3 6 R/F Dec 7/8 Research proposal presentations Research proposal presentation slides due Thu/Fri, Dec 7/8 at 1 pm
Blog post due Fri, Dec 8 at 10 pm
T Dec 12 Feedback and celebratory lunch Biomaterials engineering mini-report due Mon, Dec 11 at 10 pm
Blog post due Tue, Dec 12 at 10pm