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- 20.109(F17):Complete in silico cloning (Day1)
- 20.109(F17):Confirm gRNA sequence (Day5)
- 20.109(F17):Design gRNA for CRISPRi (Day2)
- 20.109(F17):Develop experiment to optimize loading variables (Day2)
- 20.109(F17):Evaluate cell loading results (Day3)
- 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)
- 20.109(F17):Generate gRNA plasmid (Day3)
- 20.109(F17):Growth of phage materials (Day1)
- 20.109(F17):Homework
- 20.109(F17):Induce CRISPRi system (Day7)
- 20.109(F17):Journal club II (Day 6)
- 20.109(F17):Journal club I (Day4)
- 20.109(F17):Lab tour
- 20.109(F17):Module 1
- 20.109(F17):Module 2
- 20.109(F17):Module 3
- 20.109(F17):Prepare comet chips (Day1)
- 20.109(F17):Purify active materials (Day2)
- 20.109(F17):Research proposal presentation
- 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
- 20.109(F17):Visualize active material using TEM (Day4)
- 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)
- 20.109(F17): Announcements
- 20.109(F17): Assignments
- 20.109(F17): Biomaterials engineering mini-report
- 20.109(F17): Fall 2017 Schedule
- 20.109(F17): M1 Data Summary
- 20.109(F17): M1 Mini-presentation
- 20.109(F17): Notes for M1
- 20.109(F17): Notes for orientation
- 20.109(F17): Research article
- 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)
- 20.109(F18):Assemble and test batteries (Day5)
- 20.109(F18):Class data
- 20.109(F18):Communication
- 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)
- 20.109(F18):Complete in silico cloning (Day1)
- 20.109(F18):Complete sub-nuclear foci assay (Day6)
- 20.109(F18):Confirm gRNA sequence (Day5)
- 20.109(F18):Construct cathode with phage-based active material (Day3)
- 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)
- 20.109(F18):Design gRNA for CRISPRi (Day2)
- 20.109(F18):Evaluate cell loading experiment and treat cells for genomic instability experiment (Day3)
- 20.109(F18):Generate gRNA plasmid (Day3)
- 20.109(F18):Growth of phage-based active (cathode) material (Day1)
- 20.109(F18):Homework
- 20.109(F18):Image sub-nuclear foci assay
- 20.109(F18):Image sub-nuclear foci assay (Day6)
- 20.109(F18):Induce CRISPRi system (Day7)
- 20.109(F18):Journal club presentation (Day4 and 6)
- 20.109(F18):Lab tour
- 20.109(F18):Measure fermentation products (Day8)
- 20.109(F18):Module 1
- 20.109(F18):Module 2
- 20.109(F18):Module 3
- 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)
- 20.109(F18):Practice tissue culture and prepare microwell array (Day1)
- 20.109(F18):Prep notes for M3
- 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
- 20.109(F18):Purify phage-based active (cathode) material (Day2)
- 20.109(F18):Research proposal presentation
- 20.109(F18):Visualize phage nanowires using TEM (Day4)
- 20.109(F18): Announcements
- 20.109(F18): Assignments
- 20.109(F18): Data Summary
- 20.109(F18): Fall 2018 schedule
- 20.109(F18): Mini-presentation
- 20.109(F18): Mini-report
- 20.109(F18): Prep notes for M1
- 20.109(F18): Prep notes for M2
- 20.109(F18): Research article
- 20.109(F19):Begin Western blot analysis (Day3)
- 20.109(F19):Class data
- 20.109(F19):Communication
- 20.109(F19):Complete CometChip experiment (Day5)
- 20.109(F19):Complete antibody staining for Western blot analysis (Day4)
- 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)
- 20.109(F19):Complete in silico cloning (Day1)
- 20.109(F19):Complete in silico cloning of pdCas9 (Day1)
- 20.109(F19):Confirm gRNA sequence in pgRNA(Day5)
- 20.109(F19):Confirm gRNA sequence in pgRNA (Day5)
- 20.109(F19):Data analysis and assignment preparation (Day5)
- 20.109(F19):Design gRNA for CRISPRi (Day2)
- 20.109(F19):Design gRNA sequence for CRISPRi (Day2)
- 20.109(F19):Generate gRNA plasmid (Day3)
- 20.109(F19):Guidelines for working in the tissue culture facility
- 20.109(F19):Homework
- 20.109(F19):Image CometChip (Day6)
- 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)
- 20.109(F19):Induce CRISPRi system (Day7)
- 20.109(F19):Journal club presentation (Day4 and 6)
- 20.109(F19):Lab tour
- 20.109(F19):Load cells into CometChip and apply treatments (Day4)
- 20.109(F19):Measure fermentation products (Day8)
- 20.109(F19):Module 1
- 20.109(F19):Module 2
- 20.109(F19):Module 3
- 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)
- 20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)
- 20.109(F19):Prepare for cellular thermal shift assay (Day1)
- 20.109(F19):Research proposal presentation
- 20.109(F19):Treat cells for gamma-H2AX (Day2)
- 20.109(F19): Announcements
- 20.109(F19): Assignments
- 20.109(F19): Data Summary
- 20.109(F19): Fall 2019 schedule
- 20.109(F19): Mini-presentation
- 20.109(F19): Mini-report
- 20.109(F19): Prep notes, orientation
- 20.109(F19): Prep notes for M1
- 20.109(F19): Prep notes for M2
- 20.109(F19): Prep notes for M3
- 20.109(F19): Research article
- 20.109(F20):Class data
- 20.109(F20):Communication
- 20.109(F20):Data Summary
- 20.109(F20):Guidelines for working in the tissue culture facility
- 20.109(F20):Homework
- 20.109(F20):Journal club presentation
- 20.109(F20):Lab tour
- 20.109(F20):M1D1
- 20.109(F20):M1D2
- 20.109(F20):M1D3
- 20.109(F20):M1D4
- 20.109(F20):M1D5
- 20.109(F20):M1D6
- 20.109(F20):M1D7
- 20.109(F20):M2D1
- 20.109(F20):M2D2
- 20.109(F20):M2D3
- 20.109(F20):M2D4
- 20.109(F20):M2D5
- 20.109(F20):M2D6
- 20.109(F20):M2D7
- 20.109(F20):M3D1
- 20.109(F20):M3D2
- 20.109(F20):M3D3
- 20.109(F20):M3D4
- 20.109(F20):M3D5
- 20.109(F20):Mini-presentation
- 20.109(F20):Mini-report
- 20.109(F20):Module 1
- 20.109(F20):Module 2
- 20.109(F20):Module 3
- 20.109(F20):Research article
- 20.109(F20):Research proposal presentation
- 20.109(F20): Announcements
- 20.109(F20): Assignments
- 20.109(F20): Fall 2020 schedule
- 20.109(F21):Announcements
- 20.109(F21):Assignments
- 20.109(F21):Class data
- 20.109(F21):Communication
- 20.109(F21):Data Summary
- 20.109(F21):Develop Research proposal ideas and draft presentation
- 20.109(F21):Draft research question for major assignment
- 20.109(F21):FYI
- 20.109(F21):Fall 2021 schedule
- 20.109(F21):Guidelines for working in the tissue culture facility
- 20.109(F21):Homework
- 20.109(F21):Journal club presentation
- 20.109(F21):Laboratory tour
- 20.109(F21):M1D1
- 20.109(F21):M1D2
- 20.109(F21):M1D3
- 20.109(F21):M1D4
- 20.109(F21):M1D5
- 20.109(F21):M1D6
- 20.109(F21):M1D7
- 20.109(F21):M2D1
- 20.109(F21):M2D2
- 20.109(F21):M2D3
- 20.109(F21):M2D4
- 20.109(F21):M2D5
- 20.109(F21):M2D6
- 20.109(F21):M2D7
- 20.109(F21):Module 1
- 20.109(F21):Module 2
- 20.109(F21):Organize Research article data figures and outline text
- 20.109(F21):Participate in Research proposal peer reviews
- 20.109(F21):Research article
- 20.109(F21):Research proposal presentation
- 20.109(F21):Research talk
- 20.109(F22):Assignments
- 20.109(F22):Class data
- 20.109(F22):Communication
- 20.109(F22):Data Summary
- 20.109(F22):FYI
- 20.109(F22):Fall 2022 schedule
- 20.109(F22):Guidelines for working in the tissue culture facility
- 20.109(F22):Homework
- 20.109(F22):Journal article presentation
- 20.109(F22):Laboratory tour
- 20.109(F22):M1D1
- 20.109(F22):M1D2
- 20.109(F22):M1D3
- 20.109(F22):M1D4
- 20.109(F22):M1D5
- 20.109(F22):M1D6
- 20.109(F22):M1D7
- 20.109(F22):M2D1
- 20.109(F22):M2D2
- 20.109(F22):M2D3
- 20.109(F22):M2D4
- 20.109(F22):M2D5
- 20.109(F22):M2D6
- 20.109(F22):M2D7
- 20.109(F22):M3D1
- 20.109(F22):M3D2
- 20.109(F22):M3D3
- 20.109(F22):M3D4
- 20.109(F22):Module 1
- 20.109(F22):Module 2
- 20.109(F22):Module 3
- 20.109(F22):Research article
- 20.109(F22):Research proposal presentation
- 20.109(F22):Research talk
- 20.109(F23):Assignments
- 20.109(F23):Class data
- 20.109(F23):Communication
- 20.109(F23):Data Summary
- 20.109(F23):FYI
- 20.109(F23):Fall 2023 schedule
- 20.109(F23):Guidelines for working in the tissue culture facility
- 20.109(F23):Homework
- 20.109(F23):Journal article presentation
- 20.109(F23):Laboratory tour
- 20.109(F23):M1D1
- 20.109(F23):M1D2
- 20.109(F23):M1D3
- 20.109(F23):M1D4
- 20.109(F23):M1D5
- 20.109(F23):M1D6
- 20.109(F23):M1D7
- 20.109(F23):M2D1
- 20.109(F23):M2D2
- 20.109(F23):M2D3
- 20.109(F23):M2D4
- 20.109(F23):M2D5
- 20.109(F23):M2D6
- 20.109(F23):M2D7
- 20.109(F23):M3D1
- 20.109(F23):M3D2
- 20.109(F23):M3D3
- 20.109(F23):M3D4
- 20.109(F23):Module 1
- 20.109(F23):Module 2
- 20.109(F23):Module 3
- 20.109(F23):Research article
- 20.109(F23):Research proposal presentation
- 20.109(F23):Research talk
- 20.109(S07):Assignments
- 20.109(S07):Bio-material engineering/Screening library
- 20.109(S07):General lab policy
- 20.109(S07):Guidelines for editing OpenWetWare
- 20.109(S07):Guidelines for maintaining your lab notebook
- 20.109(S07):Guidelines for oral presentations
- 20.109(S07):Guidelines for working in the tissue culture facility
- 20.109(S07):Guidelines for writing a lab report
- 20.109(S07):Lab tour
- 20.109(S07):OpenWetWare
- 20.109(S07):Start-up genome engineering
- 20.109(S07): Agarose gel electrophoresis
- 20.109(S07): Bio-material engineering/Optimizing panning
- 20.109(S07): Bio-material engineering/PCR of gold binding candidates
- 20.109(S07): Bio-material engineering/Rescreening gold binders
- 20.109(S07): Bio-material engineering/Sequence analysis
- 20.109(S07): Building phage nanowires
- 20.109(S07): Calcium signaling in vivo
- 20.109(S07): Colony PCR
- 20.109(S07): Expression engineering report
- 20.109(S07): Genome engineering essay
- 20.109(S07): Growth of phage materials
- 20.109(S07): Lipofection
- 20.109(S07): Measuring calcium in vitro
- 20.109(S07): Measuring calcium in vivo
- 20.109(S07): Microarray data analysis
- 20.109(S07): Start-up expression engineering
- 20.109(S07): Start-up signal measurement
- 20.109(S07): Student presentations, module 2
- 20.109(S07): Testing redesigned genomes
- 20.109(S07): Western analysis
- 20.109(S07): Yeast transformation
- 20.109(S07): cDNA synthesis and microarray
- 20.109(S08)
- 20.109(S08):Characterize protein expression (Day5)
- 20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)
- 20.109(S08):DNA engineering/Clean and cut DNA (Day 2)
- 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)
- 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
- 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
- 20.109(S08):DNA engineering/FACS analysis (Day 8)
- 20.109(S08):DNA engineering/Lab report
- 20.109(S08):DNA engineering/Lipofection (Day 7)
- 20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6)
- 20.109(S08):Data analysis (Day7)
- 20.109(S08):General lab policy
- 20.109(S08):Guidelines for editing OpenWetWare
- 20.109(S08):Guidelines for maintaining your lab notebook
- 20.109(S08):Guidelines for oral presentations
- 20.109(S08):Guidelines for working in the tissue culture facility
- 20.109(S08):Guidelines for writing a lab report
- 20.109(S08):Induce protein expression (Day4)
- 20.109(S08):Initiate cell culture (Day2)
- 20.109(S08):Lab basics
- 20.109(S08):Lab tour
- 20.109(S08):Module 1
- 20.109(S08):Module 2
- 20.109(S08):Module 3
- 20.109(S08):OWW basics
- 20.109(S08):OpenWetWare
- 20.109(S08):People
- 20.109(S08):Prepare expression system (Day3)
- 20.109(S08):Preparing cells for analysis (Day4)
- 20.109(S08):Protein-level analysis (Day6)
- 20.109(S08):Site-directed mutagenesis (Day2)
- 20.109(S08):Start-up biomaterials engineering (Day1)
- 20.109(S08):Start-up protein engineering (Day1)
- 20.109(S08):Student presentations (M2D8)
- 20.109(S08):Testing cell viability (Day3)
- 20.109(S08):Transcript-level analysis (Day5)
- 20.109(S08):Wrap-up analysis (Day7)
- 20.109(S08): Assignments
- 20.109(S08): Fall 2007 schedule
- 20.109(S08): Spring 2008 schedule
- 20.109(S08): TA notes for module 1
- 20.109(S08): TA notes for module 2
- 20.109(S09)
- 20.109(S09):Assay protein behavior (Day7)
- 20.109(S09):Bacterial amplification of DNA (Day3)
- 20.109(S09):Characterize protein expression (Day6)
- 20.109(S09):Data analysis (Day8)
- 20.109(S09):General lab policy
- 20.109(S09):Guidelines for maintaining your lab notebook
- 20.109(S09):Guidelines for oral presentations
- 20.109(S09):Guidelines for writing up your research
- 20.109(S09):Induce protein and evaluate DNA (Day5)
- 20.109(S09):Lab basics
- 20.109(S09):Module 1
- 20.109(S09):Module 2
- 20.109(S09):Module 3
- 20.109(S09):OpenWetWare
- 20.109(S09):People
- 20.109(S09):Prepare expression system (Day4)
- 20.109(S09):Protein-level analysis (Day6)
- 20.109(S09):Site-directed mutagenesis (Day2)
- 20.109(S09):Start-up biomaterials engineering (Day1)
- 20.109(S09):Start-up protein engineering (Day1)
- 20.109(S09):Wrap-up analysis (Day7)
- 20.109(S09): Journal article discussion (Day2)
- 20.109(S09): Luciferase assays and RNA prep (Day6)
- 20.109(S09): Microarray data analysis (Day8)
- 20.109(S09): Module 2 oral presentations
- 20.109(S09): Spring 2009 schedule
- 20.109(S09): Transfection (Day5)
- 20.109(S09): cDNA synthesis and microarray (Day7)
- 20.109(S09): siRNA design (Day1)
- 20.109(S10)
- 20.109(S10):Amplify aptamer-encoding DNA (Day1)
- 20.109(S10):Aptamer binding assay (Day7)
- 20.109(S10):Assay protein behavior (Day7)
- 20.109(S10):Bacterial amplification of DNA (Day3)
- 20.109(S10):Characterize protein expression (Day6)
- 20.109(S10):Data analysis (Day8)
- 20.109(S10):General lab policy
- 20.109(S10):Guidelines for editing OpenWetWare
- 20.109(S10):Guidelines for maintaining your lab notebook
- 20.109(S10):Guidelines for oral presentations
- 20.109(S10):Guidelines for working in the tissue culture facility
- 20.109(S10):Guidelines for writing up your research
- 20.109(S10):Induce protein and evaluate DNA (Day5)
- 20.109(S10):Initiate cell culture (Day2)
- 20.109(S10):Journal club (Day8)
- 20.109(S10):Lab basics
- 20.109(S10):Lab tour
- 20.109(S10):Module 1
- 20.109(S10):Module 2
- 20.109(S10):Module 3
- 20.109(S10):OWW basics
- 20.109(S10):OpenWetWare
- 20.109(S10):People
- 20.109(S10):Post-selection IVT and journal club (Day6)
- 20.109(S10):Prepare RNA by IVT (Day3)
- 20.109(S10):Prepare expression system (Day4)
- 20.109(S10):Preparing cells for analysis (Day4)
- 20.109(S10):Protein-level analysis (Day6)
- 20.109(S10):Purify RNA and run affinity column (Day4)
- 20.109(S10):Purify aptamer-encoding DNA (Day2)
- 20.109(S10):RNA to DNA by RT-PCR (Day5)
- 20.109(S10):Site-directed mutagenesis (Day2)
- 20.109(S10):Start-up biomaterials engineering (Day1)
- 20.109(S10):Start-up protein engineering (Day1)
- 20.109(S10):Statement on Collaboration and Integrity
- 20.109(S10):Student presentations (Day8)
- 20.109(S10):Testing cell viability (Day3)
- 20.109(S10):Transcript-level analysis (Day5)
- 20.109(S10):Wrap-up analysis (Day7)
- 20.109(S10): Assignments
- 20.109(S10): Spring 2010 schedule
- 20.109(S11)
- 20.109(S11):Amplify aptamer-encoding DNA (Day1)
- 20.109(S11):Aptamer binding assay (Day7)
- 20.109(S11):Complete DNA design (Day2)
- 20.109(S11):General lab policy
- 20.109(S11):Guidelines for editing OpenWetWare
- 20.109(S11):Guidelines for maintaining your lab notebook
- 20.109(S11):Guidelines for oral presentations
- 20.109(S11):Guidelines for working in the tissue culture facility
- 20.109(S11):Guidelines for writing up your research
- 20.109(S11):Journal club (Day8)
- 20.109(S11):Lab basics
- 20.109(S11):Lab tour
- 20.109(S11):Ligate DNA and transform bacteria (Day4)
- 20.109(S11):Module 1
- 20.109(S11):Module 2
- 20.109(S11):Module 3
- 20.109(S11):Module 3 oral presentations
- 20.109(S11):OWW basics
- 20.109(S11):OpenWetWare
- 20.109(S11):People
- 20.109(S11):Post-selection IVT and journal club (Day6)
- 20.109(S11):Prepare DNA for cloning (Day3)
- 20.109(S11):Prepare RNA by IVT (Day3)
- 20.109(S11):Purify RNA and run affinity column (Day4)
- 20.109(S11):Purify aptamer-encoding DNA (Day2)
- 20.109(S11):RNA to DNA by RT-PCR (Day5)
- 20.109(S11):Start-up biomaterials engineering (Day1)
- 20.109(S11):Statement on Collaboration and Integrity
- 20.109(S11):Student presentations (Day8)
- 20.109(S11): Assignments
- 20.109(S11): RNA computational analysis
- 20.109(S11): RNA engineering report
- 20.109(S11): Spring 2010 schedule
- 20.109(S11): TA notes for module 1
- 20.109(S11): TA notes for module 2
- 20.109(S11): TA notes for module 3
- 20.109(S12)
- 20.109(S12):Amplify aptamer-encoding DNA (Day1)
- 20.109(S12):Aptamer binding assay (Day7)
- 20.109(S12):Assess protein function (Day7)
- 20.109(S12):Bacterial amplification of DNA (Day3)
- 20.109(S12):Characterize protein expression (Day6)
- 20.109(S12):FNT summary page
- 20.109(S12):General lab policy
- 20.109(S12):Guidelines for maintaining your lab notebook
- 20.109(S12):Guidelines for oral presentations
- 20.109(S12):Guidelines for working in the tissue culture facility
- 20.109(S12):Guidelines for writing up your research
- 20.109(S12):Induce protein and evaluate DNA (Day5)
- 20.109(S12):Initiate cell culture (Day2)
- 20.109(S12):Initiating transcript and protein assays (Day5)
- 20.109(S12):Journal club (Day8)
- 20.109(S12):Lab tour
- 20.109(S12):Module 1
- 20.109(S12):Module 2
- 20.109(S12):Module 3
- 20.109(S12):Module 3 oral presentations
- 20.109(S12):OWW in 20.109
- 20.109(S12):People
- 20.109(S12):Post-selection IVT and journal club (Day6)
- 20.109(S12):Prepare RNA by IVT (Day3)
- 20.109(S12):Prepare expression system (Day4)
- 20.109(S12):Preparing cells for analysis (Day4)
- 20.109(S12):Protein-level and wrap-up analysis (Day7)
- 20.109(S12):Purify RNA and run affinity column (Day4)
- 20.109(S12):Purify aptamer-encoding DNA (Day2)
- 20.109(S12):RNA to DNA by RT-PCR (Day5)
- 20.109(S12):Reflection assignments summary page
- 20.109(S12):Site-directed mutagenesis (Day2)
- 20.109(S12):Start-up biomaterials engineering (Day1)
- 20.109(S12):Start-up protein engineering (Day1)
- 20.109(S12):Statement on collaboration and integrity
- 20.109(S12):Student presentations (Day8)
- 20.109(S12):Testing cell viability (Day3)
- 20.109(S12):Transcript-level analysis (Day6)
- 20.109(S12): Assignments
- 20.109(S12): Protein engineering report
- 20.109(S12): RNA computational analysis
- 20.109(S12): RNA engineering report
- 20.109(S12): Spring 2012 schedule
- 20.109(S12): TA notes for module 1
- 20.109(S13)
- 20.109(S13):Assess protein function (Day8)
- 20.109(S13):Bacterial amplification of DNA (Day3)
- 20.109(S13):Characterize protein expression (Day7)
- 20.109(S13):Context-setting and primer design (Day1)
- 20.109(S13):DNA cloning (Day4)
- 20.109(S13):DNA extraction (Day2)
- 20.109(S13):DNA sequencing and primer analysis (Day5)
- 20.109(S13):Design experiment (Day1)
- 20.109(S13):Design mutant (Day1)
- 20.109(S13):General lab policy
- 20.109(S13):Guidelines for maintaining your lab notebook
- 20.109(S13):Guidelines for oral presentations
- 20.109(S13):Guidelines for working in the tissue culture facility
- 20.109(S13):Guidelines for writing up your research
- 20.109(S13):Induce protein and evaluate DNA (Day5)
- 20.109(S13):Initiate cell culture (Day2)
- 20.109(S13):Initiating transcript and protein assays (Day5)
- 20.109(S13):Journal club II (Day8)
