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20.109(F14):OWW in 20.10920.109(F14):OpenWetWare20.109(F14):People
20.109(F14):Statement on collaboration and integrity20.109(F14): Assignments20.109(F14): Biomaterial engineering report
20.109(F14): DNA engineering methods20.109(F14): DNA engineering summary20.109(F14): Fall 2012 schedule
20.109(F14): Fall 2014 schedule20.109(F14): Journal Club I20.109(F14): Journal Club Presentations
20.109(F14): Mod 1 Day 1 DNA engineering using PCR20.109(F14): Mod 1 Day 2 Clean and cut DNA20.109(F14): Mod 1 Day 3 Agarose gel electrophoresis
20.109(F14): Mod 1 Day 4 DNA Ligation & Transformation20.109(F14): Mod 1 Day 4 DNA ligations, bacterial transformations and CometChip20.109(F14): Mod 1 Day 4 DNA ligations and bacterial transformations
20.109(F14): Mod 1 Day 5 Examine candidate clones & tissue culture20.109(F14): Mod 1 Day 6 Lipofection20.109(F14): Mod 1 Day 6 Lipofection and CometChip Analysis
20.109(F14): Mod 1 Day 6 Lipofection and paper discussion20.109(F14): Mod 1 Day 7 FACS analysis20.109(F14): Mod 2 Day 1 Testing an engineered biological system
20.109(F14): Mod 2 Day 2 Measuring system performance20.109(F14): Mod 2 Day 3 Tools for system engineering20.109(F14): Mod 2 Day 5 Assessing re-tuned system
20.109(F14): Mod 2 Day 6 Readouts of DNA, Protein20.109(F14): Mod 2 Day 7 Readout of Protein20.109(F14): Mod 3 Day 1 Growth of phage materials
20.109(F14): Mod 3 Day 2 Phage nanowires20.109(F14): Mod 3 Day 3 TEM20.109(F14): Mod 3 Day 4 Solar cell assembly
20.109(F14): Mod 3 Day 5 Solar cell testing20.109(F14): Mod 3 Day 6 Wrap-up and data summary20.109(F14): Mod 3 Day 7 Oral presentations
20.109(F14): Practice Virtual Lab20.109(F14): Slides for Journal Club Presentation20.109(F14): System engineering report
20.109(F14): System engineering research article20.109(F14): TA's notes for module 320.109(F14): TA notes for module 1
20.109(F14): TA notes for module 220.109(F14): TA notes for orientation20.109(F15)
20.109(F15):20.109 Class Blog summary page20.109(F15):Agarose gel electrophoresis (Day3)20.109(F15):Assess protein function (Day7)
20.109(F15):Bacterial amplification of DNA (Day3)20.109(F15):Battery assembly (Day3)20.109(F15):Battery assembly and testing (Day5)
20.109(F15):Battery testing (Day4)20.109(F15):Cathode construction (Day3)20.109(F15):Characterize protein expression (Day6)
20.109(F15):Clean and cut DNA (Day2)20.109(F15):DNA engineering using PCR (Day1)20.109(F15):DNA ligation & transformation (Day4)
20.109(F15):Evaluate mutations and site-directed mutagenesis (Day1)20.109(F15):Examine candidate clones & tissue culture (Day5)20.109(F15):FACS analysis (Day7)
20.109(F15):General lab policy20.109(F15):Growth of phage materials (Day1)20.109(F15):Guidelines for maintaining your lab notebook
20.109(F15):Guidelines for oral presentations20.109(F15):Guidelines for working in the tissue culture facility20.109(F15):Guidelines for writing up your research
20.109(F15):Homework20.109(F15):Induce protein and evaluate DNA (Day3)20.109(F15):Journal Club II (Day8)
20.109(F15):Journal Club I (Day5)20.109(F15):Lab basics20.109(F15):Lab tour
20.109(F15):Lipofection (Day6)20.109(F15):Module 120.109(F15):Module 2
20.109(F15):Module 3
20.109(F15):OWW basics
20.109(F15):People
20.109(F15):Phage nanowires (Day2)20.109(F15):Prepare expression system (Day2)20.109(F15):Purify active materials (Day2)
20.109(F15):Purify protein (Day4)20.109(F15):Research proposal presentation20.109(F15):Research proposal presentations (Day6)
20.109(F15):Site-directed mutagenesis (Day2)20.109(F15):Statement on collaboration and integrity20.109(F15):Student registration and questionnaire
20.109(F15):TEM (Day4)20.109(F15):TEM (Day5)20.109(F15):Wiki basics
20.109(F15): Assignments20.109(F15): Biomaterials engineering report20.109(F15): DNA engineering methods
20.109(F15): DNA engineering presentation20.109(F15): DNA engineering summary20.109(F15): Fall 2015 schedule
20.109(F15): Protein engineering report20.109(F15): TA's notes for module 320.109(F15): TA notes for module 1
20.109(F15): TA notes for module 220.109(F15): TA notes for orientation20.109(F16)::Measure fermentation products (Day8)
20.109(F16):Agarose gel electrophoresis (Day3)20.109(F16):Asses DNA repair variability with comet chip (Day4)20.109(F16):Battery assembly and testing (Day5)
20.109(F16):Cathode construction (Day3)20.109(F16):Clean and cut DNA (Day2)20.109(F16):Communication
20.109(F16):Complete immuno-fluorescence assay (Day6)20.109(F16):Complete in silico cloning (Day1)20.109(F16):Confirm gRNA sequence (Day5)
20.109(F16):DNA engineering using PCR (Day1)20.109(F16):DNA ligation & transformation (Day4)20.109(F16):Data analysis (Day7)
20.109(F16):Design gRNA for CRISPRi (Day2)20.109(F16):Examine candidate clones & tissue culture (Day5)20.109(F16):FACS analysis (Day7)
20.109(F16):Generate gRNA plasmid (Day3)20.109(F16):Growth of phage materials (Day1)20.109(F16):Homework
20.109(F16):Induce CRISPRi system (Day7)20.109(F16):Journal club II (Day 6)20.109(F16):Journal club I (Day4)
20.109(F16):Lab tour20.109(F16):Lipofection (Day6)20.109(F16):Measure DNA damage with comet chip (Day3)
20.109(F16):Module 120.109(F16):Module 220.109(F16):Module 3
20.109(F16):Prepare comet chips (Day1)20.109(F16):Purify active materials (Day2)20.109(F16):Research proposal presentation
20.109(F16):Seed cells for immuno-flourescence assay (Day5)20.109(F16):Statement on collaboration and integrity20.109(F16):TEM (Day4)
20.109(F16):Test comet chip assay variables (Day3)20.109(F16):Test comet chip loading variables (Day2)20.109(F16): Announcements
20.109(F16): Assignments20.109(F16): Biomaterials engineering mini-report20.109(F16): DNA engineering presentation
20.109(F16): DNA engineering summary20.109(F16): Fall 2016 schedule20.109(F16): M1 Data Summary
20.109(F16): M1 Mini-presentation20.109(F16): Research article20.109(F16): TA notes for M1
20.109(F16): TA notes for M1220.109(F16): TA notes for M220.109(F16): TA notes for M3
20.109(F16): TA notes for orientation20.109(F17)::Measure fermentation products (Day8)20.109(F17):Assemble and test batteries (Day5)
20.109(F17):Cathode construction (Day3)20.109(F17):Communication20.109(F17):Complete biochemical experiment (Day5)
20.109(F17):Complete in silico cloning (Day1)20.109(F17):Confirm gRNA sequence (Day5)20.109(F17):Design gRNA for CRISPRi (Day2)
20.109(F17):Develop experiment to optimize loading variables (Day2)20.109(F17):Evaluate cell loading results (Day3)20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)
20.109(F17):Generate gRNA plasmid (Day3)20.109(F17):Growth of phage materials (Day1)20.109(F17):Homework
20.109(F17):Induce CRISPRi system (Day7)20.109(F17):Journal club II (Day 6)20.109(F17):Journal club I (Day4)
20.109(F17):Lab tour20.109(F17):Module 120.109(F17):Module 2
20.109(F17):Module 320.109(F17):Prepare comet chips (Day1)20.109(F17):Purify active materials (Day2)
20.109(F17):Research proposal presentation20.109(F17):Test role of biochemical factors in genomic stability (Day4)20.109(F17):Visualize active material using TEM (Day4)
20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)20.109(F17): Announcements20.109(F17): Assignments
20.109(F17): Biomaterials engineering mini-report20.109(F17): Fall 2017 Schedule20.109(F17): M1 Data Summary
20.109(F17): M1 Mini-presentation20.109(F17): Notes for M120.109(F17): Notes for orientation
20.109(F17): Research article20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)20.109(F18):Assemble and test batteries (Day5)
20.109(F18):Class data20.109(F18):Communication20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)
20.109(F18):Complete in silico cloning (Day1)20.109(F18):Complete sub-nuclear foci assay (Day6)20.109(F18):Confirm gRNA sequence (Day5)
20.109(F18):Construct cathode with phage-based active material (Day3)20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)20.109(F18):Design gRNA for CRISPRi (Day2)
20.109(F18):Evaluate cell loading experiment and treat cells for genomic instability experiment (Day3)20.109(F18):Generate gRNA plasmid (Day3)20.109(F18):Growth of phage-based active (cathode) material (Day1)
20.109(F18):Homework20.109(F18):Image sub-nuclear foci assay20.109(F18):Image sub-nuclear foci assay (Day6)
20.109(F18):Induce CRISPRi system (Day7)20.109(F18):Journal club presentation (Day4 and 6)20.109(F18):Lab tour
20.109(F18):Measure fermentation products (Day8)20.109(F18):Module 120.109(F18):Module 2
20.109(F18):Module 320.109(F18):Practice statistical analysis methods and complete data analysis (Day7)20.109(F18):Practice tissue culture and prepare microwell array (Day1)
20.109(F18):Prep notes for M320.109(F18):Prepare and treat cells for genomic instability experiment (Day3)20.109(F18):Purify phage-based active (cathode) material (Day2)
20.109(F18):Research proposal presentation20.109(F18):Visualize phage nanowires using TEM (Day4)20.109(F18): Announcements
20.109(F18): Assignments20.109(F18): Data Summary20.109(F18): Fall 2018 schedule
20.109(F18): Mini-presentation20.109(F18): Mini-report20.109(F18): Prep notes for M1
20.109(F18): Prep notes for M220.109(F18): Research article20.109(F19):Begin Western blot analysis (Day3)
20.109(F19):Class data20.109(F19):Communication20.109(F19):Complete CometChip experiment (Day5)
20.109(F19):Complete antibody staining for Western blot analysis (Day4)20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)20.109(F19):Complete in silico cloning (Day1)
20.109(F19):Complete in silico cloning of pdCas9 (Day1)20.109(F19):Confirm gRNA sequence in pgRNA(Day5)20.109(F19):Confirm gRNA sequence in pgRNA (Day5)
20.109(F19):Data analysis and assignment preparation (Day5)20.109(F19):Design gRNA for CRISPRi (Day2)20.109(F19):Design gRNA sequence for CRISPRi (Day2)
20.109(F19):Generate gRNA plasmid (Day3)20.109(F19):Guidelines for working in the tissue culture facility20.109(F19):Homework
20.109(F19):Image CometChip (Day6)20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)20.109(F19):Induce CRISPRi system (Day7)
20.109(F19):Journal club presentation (Day4 and 6)20.109(F19):Lab tour20.109(F19):Load cells into CometChip and apply treatments (Day4)
20.109(F19):Measure fermentation products (Day8)20.109(F19):Module 120.109(F19):Module 2
20.109(F19):Module 320.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)
20.109(F19):Prepare for cellular thermal shift assay (Day1)20.109(F19):Research proposal presentation20.109(F19):Treat cells for gamma-H2AX (Day2)
20.109(F19): Announcements20.109(F19): Assignments20.109(F19): Data Summary
20.109(F19): Fall 2019 schedule20.109(F19): Mini-presentation20.109(F19): Mini-report
20.109(F19): Prep notes, orientation20.109(F19): Prep notes for M120.109(F19): Prep notes for M2
20.109(F19): Prep notes for M320.109(F19): Research article20.109(F20):Class data
20.109(F20):Communication20.109(F20):Data Summary20.109(F20):Guidelines for working in the tissue culture facility
20.109(F20):Homework20.109(F20):Journal club presentation20.109(F20):Lab tour
20.109(F20):M1D120.109(F20):M1D220.109(F20):M1D3
20.109(F20):M1D420.109(F20):M1D520.109(F20):M1D6
20.109(F20):M1D720.109(F20):M2D120.109(F20):M2D2
20.109(F20):M2D320.109(F20):M2D420.109(F20):M2D5
20.109(F20):M2D620.109(F20):M2D720.109(F20):M3D1
20.109(F20):M3D220.109(F20):M3D320.109(F20):M3D4
20.109(F20):M3D520.109(F20):Mini-presentation20.109(F20):Mini-report
20.109(F20):Module 120.109(F20):Module 220.109(F20):Module 3
20.109(F20):Research article20.109(F20):Research proposal presentation20.109(F20): Announcements
20.109(F20): Assignments20.109(F20): Fall 2020 schedule20.109(F21):Announcements
20.109(F21):Assignments20.109(F21):Class data20.109(F21):Communication
20.109(F21):Data Summary20.109(F21):Develop Research proposal ideas and draft presentation20.109(F21):Draft research question for major assignment
20.109(F21):FYI20.109(F21):Fall 2021 schedule20.109(F21):Guidelines for working in the tissue culture facility
20.109(F21):Homework20.109(F21):Journal club presentation20.109(F21):Laboratory tour
20.109(F21):M1D120.109(F21):M1D220.109(F21):M1D3
20.109(F21):M1D420.109(F21):M1D520.109(F21):M1D6
20.109(F21):M1D720.109(F21):M2D120.109(F21):M2D2
20.109(F21):M2D320.109(F21):M2D420.109(F21):M2D5
20.109(F21):M2D620.109(F21):M2D720.109(F21):Module 1
20.109(F21):Module 220.109(F21):Organize Research article data figures and outline text20.109(F21):Participate in Research proposal peer reviews
20.109(F21):Research article20.109(F21):Research proposal presentation20.109(F21):Research talk
20.109(F22):Assignments20.109(F22):Class data20.109(F22):Communication
20.109(F22):Data Summary20.109(F22):FYI20.109(F22):Fall 2022 schedule
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