Talk:20.109(S13): Spring 2013 schedule
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20.109(S13): Laboratory Fundamentals of Biological Engineering
Home
Schedule Spring 2013
Assignments
DNA Engineering
Protein Engineering
Cell Engineering
TEMP AGI
MODULE
DAY
DATE
LECTURER
TA
LAB
NOTES
Tue/Wed Feb 5/6
ANS
Orientation
*
Introductory Lecture (PDF)
*
Intro Pre-Lab, T/R
*
Intro Pre-Lab, W/F
1
1
Thur/Fri Feb 7/8
JR
Ian
Context-setting and primer design
*
M1D1 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
1
2
Tue/Wed Feb 12/13
JR
Ian
DNA extraction
*
M1D2 Lecture
*
T/R Pre-Lab (green text added later)
*
W/F Pre-Lab
T/R WAC talk
W/F WAC talk
1
3
Thur/Fri Feb 14/15
JR
Ian
PCR and paper discussion
*
M1D3 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
President's Day Feb 18
No lab Feb 19 or Feb 20
1
4
Thur/Fri Feb 21/22
JR
Ian
DNA cloning
*
M1D4 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
1
5
Tue/Wed Feb 26/27
JR
Ian
DNA sequencing and primer analysis
*
M1D5 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
1
6
Thur/Fri Feb 28/ Mar 1
JR
Ian
Journal club I
*
M1D6 Lecture
1
7
Tue/Wed Mar 5/6
JR
Ian
Phylogenetic analysis
*
M1D7 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
1
8
Thur/Fri Mar 7/8
NO LECTURE TODAY
Journal club II
2
1
Tue/Wed Mar 12/13
AJ
MM
Design mutant
*
M2D1 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
Module 1 Draft Due
2
2
Thur/Fri Mar 14/15
AJ
MM
Site-directed mutagenesis
*
M2D2 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
2
3
Tue/Wed Mar 19/20
AJ
MM
Bacterial amplification of DNA
*
M2D3 Lecture
*
Module 1 Primer Summary Due
*
T/R Pre-Lab
*
W/F Pre-Lab
2
4
Thur/Fri Mar 21/22
AJ
MM
Prepare expression system
*
M2D4 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
Spring Break Mar 25-29
No lab all week
2
5
Tue/Wed Apr 2/3
AJ
MM
Induce protein and evaluate DNA
*
M2D5 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
2
6
Thur/Fri Apr 4/5
AJ
MM
Purify protein
*
M2D6 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
Module 1 Revision Due
2
7
Tue/Wed Apr 9/10
AJ
MM
Characterize protein expression
*
M2D7 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
2
8
Thur/Fri Apr 11/12
AJ
MM
Assess protein function
*
M2D8 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
Patriot's Day April 15-16
No lab Apr 16 or Apr 17
3
1
Thur/Fri Apr 18/19
ANS
Design experiment
*
M3D1 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
3
2
Tue/Wed Apr 23/24
ANS
Initiate cell culture
*
M3D2 Lecture
Module 2 Report Due MONDAY APRIL 22 by 5 pm for all.
3
3
Thur/Fri Apr 25/26
ANS
*
M3D3 Lecture
Testing cell viability
3
4
Tue/Wed Apr 30/May 1
ANS
Preparing cells for analysis
*
M3D4 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
3
5
Thur/Fri May 2/3
ANS
Initiating transcript and protein assays
*
M3D5 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
3
6
Tue/Wed May 7/8
ANS
Transcript-level analysis
*
M3D6 Lecture
*
T/R Pre-Lab
*
W/F Pre-Lab
3
7
Thur/Fri May 9/10
ANS
Protein-level and wrap-up analysis
Lecture:
on Atissa's page
*
T/R Pre-Lab
*
W/F Pre-Lab
Module 3 Summary Due
3
8
Tue/Wed May 14/15
ANS
Student presentations
Thur May 16
No lab session. Meet in
56-614
from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.
Category
:
20.109ExportS13
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