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  • DNA Melting Part 1: Measuring Temperature and Fluorescence
    ...he black lead of the top device should be connected to the red lead of the bottom device. ...to the red lead of the top TEC and the black wire to the black lead on the bottom TEC using wire nuts.
    27 KB (4,497 words) - 17:49, 19 April 2017
  • DNA Melting Part 2: Lock-in Amplifier and Temperature Control
    ...to the red lead of the top TEC and the black wire to the black lead on the bottom TEC using wire nuts. # Connect the fan itself to the Fan +/- connections at the bottom of the PCB (red to +, black to -, innermost row. See figure).
    20 KB (3,227 words) - 20:05, 7 November 2017
  • DNA Melting: Simulating DNA Melting - Intermediate Topics
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    9 KB (1,281 words) - 21:46, 17 August 2017
  • Lab Manual: Optical Microscopy
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    23 KB (3,767 words) - 15:19, 10 January 2017
  • Electronics primer
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    22 KB (3,534 words) - 13:50, 19 October 2018
  • Lab Manual: Atomic Force Microscopy (AFM)
    ...12 &mu;m area, at a rate of one line per second, and is currently near the bottom of the image.</figure>]] ... the Y-scan direction tells the scanner whether to start at the top or the bottom of an image, and the trace/retrace selector determines whether each line is
    56 KB (9,320 words) - 20:42, 13 December 2017
  • 20.109(S16):Induce protein expression (Day5)
    #*At the bottom right should be a link to download your sequencing results.
    16 KB (2,609 words) - 21:55, 23 February 2016
  • Fall 2010 Syllabus
    |+ align="bottom" style="color:#e76700;" |''Food complements''
    266 B (28 words) - 00:57, 16 September 2010
  • Procedure: Diffusion in biological gels
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    5 KB (878 words) - 23:26, 5 October 2010
  • 20.345:Course Information
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    11 KB (1,722 words) - 17:22, 6 February 2018
  • Spring 2011:Optical Trapping Lab
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    10 KB (1,595 words) - 16:29, 28 February 2012
  • Confocal Wiki
    ...e the desired name of your image file (.tif) in the imwrite section at the bottom of the code.
    21 KB (3,664 words) - 21:38, 4 June 2015
  • Theory
    ... (B, in electron/(pixel second)). The number of cycles is indicated at the bottom of each table. It has to be considered though that the number of cycles is
    9 KB (1,442 words) - 20:08, 19 May 2011
  • Results
    If not stated at the bottom right corner of each image, the parameters used for the experiment were SNR
    2 KB (338 words) - 20:30, 19 May 2011
  • DNA Melting: Processing DNA Melting Data
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    7 KB (1,108 words) - 17:58, 8 October 2011
  • DNA Melting Report Requirements for Part 1
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    2 KB (393 words) - 21:34, 23 March 2017
  • Status Report Interrogatories (Spring 2012)
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    4 KB (610 words) - 18:44, 13 August 2013
  • Spring 2012:Leanna Morinishi
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    2 KB (249 words) - 17:50, 18 November 2013
  • Spring 2012:Leanna Morinishi Lab 1
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    13 KB (2,020 words) - 19:56, 9 March 2012
  • Spring 2012:Leanna Morinishi Journal
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    14 KB (2,366 words) - 04:50, 17 May 2012
  • Spring 2012:Vincent Lee Journal
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    8 KB (1,373 words) - 18:43, 13 August 2013
  • Spring 2012: Jonathan Gootenberg Journal
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    11 KB (1,937 words) - 22:57, 22 May 2012
  • 20.345:Key Factors Table
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    2 KB (306 words) - 01:05, 21 March 2012
  • Spring 2012:LFM
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    4 KB (576 words) - 05:19, 18 May 2012
  • Understanding the lock-in amplifier
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    15 KB (1,965 words) - 16:36, 13 November 2017
  • Optical Microscopy Part 4: Particle Tracking
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    9 KB (1,469 words) - 01:01, 10 March 2016
  • Protocols for cell culture
    * Water at the bottom: * Blower always on, vented at the bottom (20.109 students, beware: this type of hood is different from regular ones
    18 KB (2,896 words) - 18:17, 25 February 2024
  • Protocols
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    4 KB (644 words) - 18:24, 15 August 2013
  • Optical Microscopy Part 1: Brightfield Microscopy
    .... Also, note that the stages are very expensive; always lift them from the bottom. ... Week 1 report: (top) Air Force target, (center) Silica spheres and dust, (bottom) Ronchi Ruling]]
    23 KB (3,758 words) - 22:45, 12 January 2017
  • Aligning the optical trap
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    4 KB (645 words) - 22:09, 3 May 2013
  • Optical Microscopy Part 2: Fluorescence Microscopy
    ...he day prior to the fluorescence imaging, cells were plated on 35 mm glass-bottom cell culture dishes (MatTek, equipped with coverslip suited for optical mic ...ple images and histogram. Top: fluorescent beads; center: reference slide, bottom: image histogram.]]
    20 KB (3,262 words) - 22:06, 14 August 2017
  • Optical detectors, noise, and the limit of detection
    ===Bottom line on optical detector noise=== {{Template:20.309 bottom}}
    14 KB (2,102 words) - 13:42, 3 March 2021
  • Error analysis
    ...ial configuration to be as insensitive as possible to such effects" </ref> Bottom line: systematic errors are much harder to characterize than random errors. ...g the subject of measurement error seem more mysterious than it really is. Bottom line: Precision quantifies the variability of a measurement. Accuracy speci
    24 KB (3,757 words) - 14:57, 3 September 2018
  • Optics Bootcamp
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    28 KB (4,857 words) - 20:09, 31 July 2017
  • 20.109(S08):Testing cell viability (Day3)
    ... over (to the right). This is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis. #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl
    18 KB (3,087 words) - 16:04, 15 June 2015
  • 20.109(S08):Initiate cell culture (Day2)
    ...n body inside this sterile area. (By back wall I mean what will become the bottom inside wall when you lay the flask down, which is the surface on which your
    12 KB (2,006 words) - 16:04, 15 June 2015
  • 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)
    ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
    27 KB (3,752 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Clean and cut DNA (Day 2)
    ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
    10 KB (1,692 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)
    ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
    10 KB (1,806 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
    ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
    10 KB (1,704 words) - 14:24, 5 June 2015
  • 20.109(S08):DNA engineering/Examine candidate clones (Day 5)
    ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
    10 KB (1,634 words) - 14:24, 5 June 2015
  • 20.109(S08):Site-directed mutagenesis (Day2)
    (*)<small>In a 14 mL polypropylene round-bottom tube, 1 &mu;L of DNA will be added to 50 &mu;L of competent cells, and the
    12 KB (1,954 words) - 14:24, 5 June 2015
  • 20.109(S08):Lab tour
    ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
    22 KB (3,631 words) - 14:24, 5 June 2015
  • 20.109(S07):Lab tour
    ...our P20, measure 10, 15 and 20 l of the 0.01% XC stock solution into the bottom of three cuvettes. Using your P1000, add water to bring the final volume to ...r P200, measure 20, 50 and 100 l of the 0.01% XC stock solution into the bottom of three more cuvettes. Using your P1000, add water to bring the final volu
    22 KB (3,724 words) - 15:32, 15 June 2015
  • 20.109(S07): Start-up expression engineering
    ... sequence and whether you have the "top" strand as you did before, or the "bottom" strand as you will need for this reverse primer. [[Image:Macintosh HD-User
    28 KB (4,311 words) - 15:32, 15 June 2015
  • Bryan Hernandez/20.109/Lab notebook/Module 1/Day 3
    ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
    12 KB (2,079 words) - 15:32, 15 June 2015
  • Bryan Hernandez/20.109/Lab notebook/Module 2/Day 2
    7. Wipe the top and bottom sensor with a water soaked Chemwipe.<br> 8. Dry the top and bottom sensor with a dry Chemwipe.<br>
    19 KB (3,069 words) - 15:48, 15 June 2015
  • 20.109(S07):Bio-material engineering/Screening library
    9. After panning for 30 minutes, move the gold slides to the bottom three wells of the six well dish with 2 ml fresh Gal Blocking/Binding Buffe
    9 KB (1,473 words) - 15:31, 15 June 2015
  • Bryan Hernandez/20.109/Lab notebook/Module 1/Day 4
    ...or, but be careful not to disturb the pellet of plasmid DNA that is at the bottom of the tube. Remove as much of the supernatant as possible but you do not n ... to completely dissolve the pellets. The liquid can be brought back to the bottom of the tubes by spinning them in the microfuge for a few seconds. Store the
    11 KB (1,751 words) - 15:31, 15 June 2015
  • 20.109(S07):Start-up genome engineering
    ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example the recognition sequence for EcoRI is ...and give it a quick spin in the microfuge to bring any pellets down to the bottom of the eppendorf tube (be sure to balance your tube against another in the
    32 KB (5,419 words) - 15:31, 15 June 2015

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