20.109(S19):Class data
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Module 1: Assessing ligand binding
M1D3: SMM analysis
Files for SMM analysis
GAL files linked here.
Scan images and set numbers assigned to each team:
| Team | Slide #1 | Slide #2 |
| T/R | ||
| Red | 14399983, set 1 | 14400077, set 4 |
| Orange | 14400113, set 2 | 14400139, set 3 |
| Yellow | 14400114, set 2 | 14400140, set 3 |
| Green | 14399984, set 5 | 14399988, set 6 |
| Blue | 14400072, set 5 | 14399987, set 6 |
| Pink | 14399983, set 1 | 14400128, set 7 |
| Purple | 14400077, set 4 | 14399959, set 8 |
| White | 14400129, set 7 | 14400081, set 9 |
| Grey | 14400128, set 7 | 14399959, set 8 |
| W/F | ||
| Red | 14400129, set 7 | 14400081, set 9 |
| Orange | 14400128, set 7 | 14399959, set 8 |
| Yellow | 14400143, set 11 | 14399950, set 12 |
| Green | 14400147, set 10 | 14399951, set 12 |
| Blue | 14400146, set 10 | 14399947, set 11 |
| Pink | 14399983, set 1 | 14400077, set 4 |
| Grey | 14400113, set 2 | 14400139, set 3 |
Ligand selections
| Team | Ligand #1 (short #) | Ligand #2 (short #) |
| T/R | ||
| Red | 31064975 (15) | 13742946 (16) |
| Orange | 83759472 (2) | 34120495 (13) |
| Yellow | 83759472 (2) | 64183606 (5) |
| Green | 85836506 (1) | 41507070 (11) |
| Blue | 71743840 (14) | 64472564 (4) |
| Pink | 47464042 (8) | 85836506 (1) |
| Purple | 47464042 (8) | 63365116 (6) |
| White | 62716075 (7) | 45527639 (10) |
| Grey | 62716075 (7) | 64472564 (4) |
| W/F | ||
| Red | 41507070 (11) | 13742946 (16) |
| Orange | 34297777 (12) | 64183606 (5) |
| Yellow | 45714400 (9) | 31064975 (15) |
| Green | 34120495 (13) | 71743840 (14) |
| Blue | 65421147 (3) | 45527639 (10) |
| Pink | 63365116 (6) | 45714400 (9) |
| Grey | 41507070 (11) | 65421147 (3) |
M1D3: Evaluate protein purity and concentration
WF Section
BCA Assay results (A562 measurements): linked here
SDS-PAGE images:
Red Team
Orange Team
Yellow Team
Green Team
Blue Team
Pink Team
Grey Team
M1D5: PPIase assay
TR Section
Plate #1: linked here
Plate #2: linked here
WF Section
Plate #1: linked here
Plate #2: linked here
Plate #3: linked here
M1D6: DSF results
TR Section
First Derivative: linked here
Melt Curve: linked here
WF Section
First Derivative: linked here
Melt Curve: linked here
M1D7: Data analysis
| Team | PPIase results | DSF results |
| T/R | ||
| Red | DSF red | PPiase red |
| Orange | DSF orange | PPiase orange |
| Yellow | DSF yellow | PPiase yellow |
| Green | DSF green | PPiase green |
| Blue | DSF blue | PPiase blue |
| Pink | DSF pink | PPiase pink |
| Purple | DSF purple | PPiase purple |
| White | DSF white | PPiase white |
| Platinum | DSF platinum | PPiase platinum |
| W/F | ||
| Red | ||
| Orange | ||
| Yellow | PPiase Yellow | |
| Green | DSF Green | |
| Blue | ||
| Pink | ||
| Grey | ||
