20.309 Main Page

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20.309 This week and next in the 20.309 lab

Week	Summary	Assignments due	Lab Manual	Resources
Week 8: Oct. 23 - 29	Finish Microscopy 3, study for Exam 1, and if you're super keen, get started on the Electronics bootcamp!	Tuesday Oct. 25: Microscopy 3 Thursday Oct.27: Exam 1 (at 7:30pm in 4-163)	Microscopy 3	Overview Lab Manual: Optical Microscopy Code examples and simulations Converting Gaussian fit to Rayleigh resolution MATLAB: Estimating resolution from a PSF slide image Matlab: Scalebars Calculating MSD and Diffusion Coefficients Background reading Geometrical optics and ray tracing Physical optics and resolution Optical aberrations Aperture and field stops Optical detectors, noise, and the limit of detection Manta G032 camera measurements
			Electronics bootcamp	Electronics primer Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider" Real electronics Impedance analysis Transfer functions and Bode plots Complex number review Input and output impedance
Week 9: Oct. 30 - Nov. 5	Complete the electronics bootcamp	Friday Nov. 4: Electronics Bootcamp	Electronics bootcamp	* Electronics primer Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider" Real electronics Impedance analysis Transfer functions and Bode plots Complex number review Input and output impedance

20.309 course information

• 20.309:Course Information
• 20.309:Lab Report Guidelines
• 20.309:Safety

Reserve a lab station here: Bioinstrumentation Lab reservation system

Optics bootcamp

Lab manual

• Optics Bootcamp

Background reading

• Geometrical optics and ray tracing
• Optical detectors, noise, and the limit of detection
• Manta G032 camera measurements

Optical microscopy lab

Code examples and simulations

• Converting Gaussian fit to Rayleigh resolution
• MATLAB: Estimating resolution from a PSF slide image
• Matlab: Scalebars
• Calculating MSD and Diffusion Coefficients

Background reading

• Geometrical optics and ray tracing
• Physical optics and resolution
• Optical aberrations
• Aperture and field stops
• Optical detectors, noise, and the limit of detection
• Manta G032 camera measurements
• Understanding log plots

Electronics bootcamp

Lab manual

• Electronics bootcamp

Background reading

• Electronics primer
• Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider"
• Real electronics
• Impedance analysis
• Transfer functions and Bode plots
• Complex number review
• Input and output impedance

DNA melting lab

Lab manuals

• Lab Manual:Measuring DNA Melting Curves
• DNA Melting Part 1: Measuring Temperature and Fluorescence
• DNA Melting Report Requirements for Part 1
• DNA Melting: Model function and parameter estimation by nonlinear regression
• DNA Melting: Simulating DNA Melting - Intermediate Topics
• DNA melting: Identifying the unknown sample
• DNA Melting Part 2: Lock-in Amplifier and Temperature Control
• DNA Melting Report Requirements for Part 2

Code examples and simulations

• DNA Melting: Simulating DNA Melting - Basics
• DNA Melting: Simulating DNA Melting - Intermediate Topics
• DNA Melting: Model function and parameter estimation by nonlinear regression

Reference materials

• DNA Melting Thermodynamics
• DNA Melting: DNA Sequences
• DINAMelt Web Server

Background reading

• Electronics Primer

Limits of detection mini-lab

• Lab Manual: Limits of Detection
• Lab Manual: Atomic Force Microscopy (AFM)
• Limits of Detection: Data Sessions

MRI mini-lab

• MGH tabletop MRI

(Kept for historical reasons.)