Difference between revisions of "20.309 Main Page"
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|style="width: 35%;"|'''Resources''' | |style="width: 35%;"|'''Resources''' | ||
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| − | + | | Week 4: Feb. 26 - Mar. 4 | |
| − | + | | Finish Microscopy 2 | |
| − | + | | <font color=#04B45F> | |
| − | | [[Optical Microscopy Part | + | *Sunday Feb. 26: PSet 1 |
| + | *Friday Mar. 3: Microscopy 2 due</font color> | ||
| + | | [[Optical Microscopy Part 2: Fluorescence Microscopy|Microscopy 2]] (Remember to reserve your cells [http://doodle.com/poll/u4re67r7wmtcihyu here]!) | ||
| Overview | | Overview | ||
* [[Lab Manual: Optical Microscopy]] | * [[Lab Manual: Optical Microscopy]] | ||
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* [[Optical detectors, noise, and the limit of detection]] | * [[Optical detectors, noise, and the limit of detection]] | ||
* [[Manta G032 camera measurements]] | * [[Manta G032 camera measurements]] | ||
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| − | + | ||
|-- | |-- | ||
| − | | Week | + | | Week 5: Mar. 5 - 11 |
| − | | | + | | Prepare for Quiz 1, Start working on PSet 2 and Microscopy 3 |
| − | | <font color=#04B45F> | + | | <font color=#04B45F> Thursday Mar. 9: Quiz 1 in lecture</font color> |
| − | + | | [[Optical Microscopy Part 3: Resolution, Stability, and Particle Tracking|Microscopy 3]] | |
| − | + | | | |
| − | | [[Optical Microscopy Part | + | |
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20.309 This week and next in the 20.309 lab
| Week | Summary | Assignments due | Lab Manual | Resources |
| Week 4: Feb. 26 - Mar. 4 | Finish Microscopy 2 |
|
Microscopy 2 (Remember to reserve your cells here!) | Overview
Code examples and simulations
Background reading |
| Week 5: Mar. 5 - 11 | Prepare for Quiz 1, Start working on PSet 2 and Microscopy 3 | Thursday Mar. 9: Quiz 1 in lecture | Microscopy 3 |
Contents
20.309 course information
Reserve a lab station here: Bioinstrumentation Lab reservation system
Optics bootcamp
Lab manual
Background reading
- Geometrical optics and ray tracing
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
Background reading
- Geometrical optics and ray tracing
- Physical optics and resolution
- Optical aberrations
- Aperture and field stops
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
- Understanding log plots
Electronics bootcamp
Lab manual
Background reading
- Electronics primer
- Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider"
- Real electronics
- Impedance analysis
- Transfer functions and Bode plots
- Complex number review
- Input and output impedance
DNA melting lab
Lab manuals
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Model function and parameter estimation by nonlinear regression
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA melting: Identifying the unknown sample
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
Code examples and simulations
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting: Model function and parameter estimation by nonlinear regression
Reference materials
Background reading
Limits of detection mini-lab
- Lab Manual: Limits of Detection
- Lab Manual: Atomic Force Microscopy (AFM)
- Limits of Detection: Data Sessions
