Difference between revisions of "20.309 Main Page"
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| − | | Week | + | | rowspan="2"|Week 2: Feb. 12 - 18 |
| − | | | + | | rowspan="2"|Finish the Optics Bootcamp and start Microscopy 1 |
| − | | | + | | rowspan="2"|<font color=#04B45F>'''Feb. 13 (Mon): Optics Bootcamp'''</font color> |
| − | | [[Optics Bootcamp]] | + | | [[Optics Bootcamp]] continued (Sun-Mon) |
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* [[Geometrical optics and ray tracing]] | * [[Geometrical optics and ray tracing]] | ||
* [[Optical detectors, noise, and the limit of detection]] | * [[Optical detectors, noise, and the limit of detection]] | ||
* [[Manta G032 camera measurements]] | * [[Manta G032 camera measurements]] | ||
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|- | |- | ||
| − | | [[Optical Microscopy Part 1: Brightfield Microscopy|Microscopy 1]] | + | | [[Optical Microscopy Part 1: Brightfield Microscopy|Microscopy 1]] |
| Overview | | Overview | ||
* [[Lab Manual: Optical Microscopy|Optical Microscopy Overview]] | * [[Lab Manual: Optical Microscopy|Optical Microscopy Overview]] | ||
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* [[Optical detectors, noise, and the limit of detection]] | * [[Optical detectors, noise, and the limit of detection]] | ||
* [[Manta G032 camera measurements]] | * [[Manta G032 camera measurements]] | ||
| − | + | |-- | |
| + | | rowspan="2"| Week 3: Feb. 19 - 25 | ||
| + | | rowspan="2"|Finish Microscopy 1, start working on Microscopy 2 and PSet 1 | ||
| + | | rowspan="2"|<font color=#04B45F>'''Feb. 20 (Mon): Microscopy 1'''</font color> | ||
| + | | | [[Optical Microscopy Part 1: Brightfield Microscopy|Microscopy 1]] continued (Sun-Mon) | ||
| + | | | ||
| + | |- | ||
| + | | [[Optical Microscopy Part 2: Fluorescence Microscopy|Microscopy 2]] (Tues-Sat) | ||
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Revision as of 18:50, 12 February 2017
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20.309 This week and next
| Week | Summary | Assignments due | Lab Manual | Resources |
| Week 2: Feb. 12 - 18 | Finish the Optics Bootcamp and start Microscopy 1 | Feb. 13 (Mon): Optics Bootcamp | Optics Bootcamp continued (Sun-Mon) | |
| Microscopy 1 | Overview
Code examples and simulations
Background reading | |||
| Week 3: Feb. 19 - 25 | Finish Microscopy 1, start working on Microscopy 2 and PSet 1 | Feb. 20 (Mon): Microscopy 1 | Microscopy 1 continued (Sun-Mon) | |
| Microscopy 2 (Tues-Sat) |
Contents
20.309 course information
Reserve a lab station here: Bioinstrumentation Lab reservation system
Optics bootcamp
Lab manual
Background reading
- Geometrical optics and ray tracing
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
Background reading
- Geometrical optics and ray tracing
- Physical optics and resolution
- Optical aberrations
- Aperture and field stops
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
- Understanding log plots
Electronics bootcamp
Lab manual
Background reading
- Electronics primer
- Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider"
- Real electronics
- Impedance analysis
- Transfer functions and Bode plots
- Complex number review
- Input and output impedance
DNA melting lab
Lab manuals
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Model function and parameter estimation by nonlinear regression
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA melting: Identifying the unknown sample
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
Code examples and simulations
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting: Model function and parameter estimation by nonlinear regression
Reference materials
Background reading
Limits of detection mini-lab
- Lab Manual: Limits of Detection
- Lab Manual: Atomic Force Microscopy (AFM)
- Limits of Detection: Data Sessions
