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|style="width: 17%;"|'''Lab Manual''' | |style="width: 17%;"|'''Lab Manual''' | ||
|style="width: 35%;"|'''Resources''' | |style="width: 35%;"|'''Resources''' | ||
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| Week 10: Nov. 6 - Nov. 12 | | Week 10: Nov. 6 - Nov. 12 | ||
| Start DNA Melting Part 1 | | Start DNA Melting Part 1 | ||
| − | | <font color=#04B45F> | + | | <font color=#04B45F> Thursday Nov. 10th: Quiz 2 (during lecture)</font color> |
| [[DNA Melting Part 1: Measuring Temperature and Fluorescence]] | | [[DNA Melting Part 1: Measuring Temperature and Fluorescence]] | ||
[[DNA Melting Report Requirements for Part 1]] | [[DNA Melting Report Requirements for Part 1]] | ||
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Background reading | Background reading | ||
* [[Electronics Primer]] | * [[Electronics Primer]] | ||
| − | <!-- * [[Real electronics]] -->|} | + | <!-- * [[Real electronics]] -->| |
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| + | | Week 11: Nov. 13 - Nov. 19 | ||
| + | | Finish DNA Melting Part 1 | ||
| + | | <font color=#04B45F> Friday Nov. 18th: DNA Melting Part 1</font color> | ||
| + | | [[DNA Melting Part 1: Measuring Temperature and Fluorescence]] | ||
| + | [[DNA Melting Report Requirements for Part 1]] | ||
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20.309 This week and next in the 20.309 lab
| Week | Summary | Assignments due | Lab Manual | Resources | |
| Week 10: Nov. 6 - Nov. 12 | Start DNA Melting Part 1 | Thursday Nov. 10th: Quiz 2 (during lecture) | DNA Melting Part 1: Measuring Temperature and Fluorescence | Overview
Code examples and simulations
Reference materials Background reading |
|
| Week 11: Nov. 13 - Nov. 19 | Finish DNA Melting Part 1 | Friday Nov. 18th: DNA Melting Part 1 | DNA Melting Part 1: Measuring Temperature and Fluorescence |
Contents
20.309 course information
Reserve a lab station here: Bioinstrumentation Lab reservation system
Optics bootcamp
Lab manual
Background reading
- Geometrical optics and ray tracing
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
Background reading
- Geometrical optics and ray tracing
- Physical optics and resolution
- Optical aberrations
- Aperture and field stops
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
- Understanding log plots
Electronics bootcamp
Lab manual
Background reading
- Electronics primer
- Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider"
- Real electronics
- Impedance analysis
- Transfer functions and Bode plots
- Complex number review
- Input and output impedance
DNA melting lab
Lab manuals
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Model function and parameter estimation by nonlinear regression
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA melting: Identifying the unknown sample
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
Code examples and simulations
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting: Model function and parameter estimation by nonlinear regression
Reference materials
Background reading
Limits of detection mini-lab
- Lab Manual: Limits of Detection
- Lab Manual: Atomic Force Microscopy (AFM)
- Limits of Detection: Data Sessions
