20.309 Main Page
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20.309 course information
- 20.309 course information
- lab safety
- Reserve a lab station here: Bioinstrumentation Lab reservation system
20.309 master schedule
Module 1: Optics & Microscopy
Important dates | Assignment due | Assignment page links | Useful resources |
9/16/18 | Assignment 1 |
| |
9/23/18 | Assignment 2 | Assignment 2 Overview | |
9/27/18 | Quiz 1 (in lecture) | ||
9/30/18 | Assignment 3 | Assignment 3 Overview | |
10/7/18 | Assignment 4 | ||
10/14/18 | Assignment 5 | Assignment 5 Overview | |
10/18/18 | Exam 1 (at 7:30pm in 35-225) |
Module 2: Signals and Systems
Important dates | Assignment due | Assignment page links | Useful resources |
10/28/18 | Assignment 6 | Electronics bootcamp I: RC filters, transfer functions, nonlinear fitting | |
11/4/18 | Assignment 7 | Electronics bootcamp II | |
11/9/18 | Quiz 2 (in lecture) | ||
11/11/18 | Assignment 8 | Flow channel & two-color microscope construction | |
11/18/18 | Assignment 9 | Analysis code | |
12/2/18 | Assignment 10 | Collect data | |
12/6/18 | Exam 2 (at 7:30pm in 35-225) | ||
12/11/18 | Assignment 11 | Present lab visit |
Optics and microscopy resources
Background reading
- Geometrical optics and ray tracing
- Physical optics and resolution
- Optical aberrations
- Aperture and field stops
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
- Understanding log plots
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
Electronics resources
Background reading
- Electronics primer
- Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider"
- Real electronics
- Impedance analysis
- Transfer functions and Bode plots
- Complex number review
- Input and output impedance
Code examples and simulations
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting: Model function and parameter estimation by nonlinear regression
Reference materials
Limits of detection mini-lab
- Lab Manual: Limits of Detection
- Lab Manual: Atomic Force Microscopy (AFM)
- Limits of Detection: Data Sessions