20.109(S23):Module 2

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20.109(S23): Laboratory Fundamentals of Biological Engineering

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Spring 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Module 2: protein engineering

Research goal:


[[Image:|center|750px|thumb|Image generated using BioRender.]]



Lab links: day by day

M2D1: Determine mutagenesis strategy
M2D2: Perform site-directed mutagenesis
M2D3: Sequence clones and transform into yeast cells
M2D4: Determine transporter mutation and prepare metal uptake experiment
M2D5: Analyze ICP-OES data
M2D6: IF staining and prepare for cytotoxicity experiment
M2D7: Confirm transporter expression and cell survival of yeast exposed to metal
M2D8: Complete data analysis and organize Research article figures

Major assignments

Journal article presentation
Research article

References

Notes for Instructors

Prep notes for M2
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