Difference between revisions of "20.109(S23):Module 2"
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| + | ==Module 2: protein engineering== | ||
<font color= #015526 >'''Research goal: '''</font color> | <font color= #015526 >'''Research goal: '''</font color> | ||
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| + | [[Image:|center|750px|thumb|Image generated using BioRender.]] | ||
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| + | <br style="clear:both;"/> | ||
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| + | ==Lab links: day by day== | ||
| + | M2D1: [[20.109(S23):M2D1 |Determine mutagenesis strategy]] <br> | ||
| + | M2D2: [[20.109(S23):M2D2 |Perform site-directed mutagenesis]] <br> | ||
| + | M2D3: [[20.109(S23):M2D3 |Sequence clones and transform into yeast cells]] <br> | ||
| + | M2D4: [[20.109(S23):M2D4 |Determine transporter mutation and prepare metal uptake experiment]] <br> | ||
| + | M2D5: [[20.109(S23):M2D5 | Analyze ICP-OES data]] <br> | ||
| + | M2D6: [[20.109(S23):M2D6 | IF staining and prepare for cytotoxicity experiment]] <br> | ||
| + | M2D7: [[20.109(S23):M2D7 |Confirm transporter expression and cell survival of yeast exposed to metal]] <br> | ||
| + | M2D8: [[20.109(S23):M2D8 |Complete data analysis and organize Research article figures]] <br> | ||
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| + | ==Major assignments== | ||
| + | [[20.109(S23):Journal article presentation| Journal article presentation]] <br> | ||
| + | [[20.109(S23):Research article|Research article]] <br> | ||
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| + | ==References== | ||
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| + | * | ||
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| + | ==Notes for Instructors== | ||
| + | [[20.109(S23): Prep notes for M2| Prep notes for M2]] | ||
Revision as of 19:40, 5 February 2023
Contents
Module 2: protein engineering
Research goal:
[[Image:|center|750px|thumb|Image generated using BioRender.]]
Lab links: day by day
M2D1: Determine mutagenesis strategy
M2D2: Perform site-directed mutagenesis
M2D3: Sequence clones and transform into yeast cells
M2D4: Determine transporter mutation and prepare metal uptake experiment
M2D5: Analyze ICP-OES data
M2D6: IF staining and prepare for cytotoxicity experiment
M2D7: Confirm transporter expression and cell survival of yeast exposed to metal
M2D8: Complete data analysis and organize Research article figures
Major assignments
Journal article presentation
Research article
