User contributions
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- 17:05, 2 February 2023 (diff | hist) . . (0) . . N File:PYES2-CT vector map.png (current)
- 22:36, 1 February 2023 (diff | hist) . . (-421) . . 20.109(S23):M2D2 (→Part 3: Use site directed mutagenesis to introduce point mutations into Fet4 transporter)
- 22:36, 1 February 2023 (diff | hist) . . (-83) . . 20.109(S23):M2D2 (→Reagents list)
- 22:35, 1 February 2023 (diff | hist) . . (-148) . . 20.109(S23):M2D2 (→Part 3: Use site directed mutagenesis to introduce point mutations into Fet4 transporter)
- 22:27, 1 February 2023 (diff | hist) . . (+444) . . 20.109(S23):M2D2 (→Part 3: Insert sgRNA_target sequence into expression plasmid)
- 22:12, 1 February 2023 (diff | hist) . . (-320) . . 20.109(S23):M2D2 (→Part 2: Prepare Fet4_mutant oligo)
- 22:05, 1 February 2023 (diff | hist) . . (-1) . . 20.109(S23):M2D2 (→Part 2: Prepare sgRNA_target oligo)
- 22:04, 1 February 2023 (diff | hist) . . (-353) . . 20.109(S23):M2D2 (→Part 1: Research sgRNA expression plasmid)
- 21:37, 1 February 2023 (diff | hist) . . (-1) . . 20.109(S23):M2D3 (→Part 1: Mini-prep pgRNA_target clones)
- 21:35, 1 February 2023 (diff | hist) . . (-4) . . 20.109(S23):M2D3 (→Part 1: Mini-prep pgRNA_target clones)
- 21:31, 1 February 2023 (diff | hist) . . (+125) . . 20.109(S23):M2D3 (→Reagents list)
- 21:21, 1 February 2023 (diff | hist) . . (-96) . . 20.109(S23):M2D3 (→Reagents list)
- 21:20, 1 February 2023 (diff | hist) . . (-71) . . 20.109(S23):M2D3 (→Part 3: Prepare psgRNA clones for sequencing analysis)
- 21:10, 1 February 2023 (diff | hist) . . (0) . . 20.109(S23):M2D3 (→Protocols)
- 21:10, 1 February 2023 (diff | hist) . . (-187) . . 20.109(S23):M2D3 (→Protocols)
- 21:09, 1 February 2023 (diff | hist) . . (-671) . . 20.109(S23):M2D3 (→Part 3: Transform mutated plasmid into W303α yeast cells)
- 20:41, 1 February 2023 (diff | hist) . . (+1) . . 20.109(S23):M2D3 (→Part 3: Transform CRIPSRi system into W303α yeast cells)
- 20:40, 1 February 2023 (diff | hist) . . (-188) . . 20.109(S23):M2D3 (→Part 3: Transform CRIPSRi system into W303α yeast cells)
- 19:53, 1 February 2023 (diff | hist) . . (+452) . . 20.109(S23):M2D3 (→Part 3: Transform CRIPSRi system into W303α yeast cells)
- 19:45, 1 February 2023 (diff | hist) . . (-2) . . 20.109(S23):M2D3 (→Part 3: Transform CRIPSRi system into MG1655 E. coli cells)
- 19:44, 1 February 2023 (diff | hist) . . (+2) . . 20.109(S23):M2D3 (→Part 3: Transform CRIPSRi system into W303α cells)
- 19:22, 1 February 2023 (diff | hist) . . (+3) . . 20.109(S23):Spring 2023 schedule
- 19:15, 1 February 2023 (diff | hist) . . (-38) . . 20.109(S23):Spring 2023 schedule
- 19:03, 1 February 2023 (diff | hist) . . (+1,018) . . 20.109(S23):M2D2
- 19:01, 1 February 2023 (diff | hist) . . (+3,061) . . 20.109(S23):M2D1 (→Protocols)
- 22:27, 31 January 2023 (diff | hist) . . (+10) . . 20.109(S23):M2D1
- 22:26, 31 January 2023 (diff | hist) . . (+178) . . 20.109(S23):M2D2
- 22:25, 31 January 2023 (diff | hist) . . (+201) . . 20.109(S23):M2D3
- 22:23, 31 January 2023 (diff | hist) . . (+168) . . 20.109(S23):M2D4
- 22:21, 31 January 2023 (diff | hist) . . (+333) . . N 20.109(S23):M2D5 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}} ==Introduction== ==Navigation links== Next day: 20.109(S23):M2D6 |Determin...")
- 22:20, 31 January 2023 (diff | hist) . . (+310) . . N 20.109(S23):M2D6 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}} ==Introduction== ==Navigation links== Next day: 20.109(S23):M2D7 |Confirm ...")
- 22:18, 31 January 2023 (diff | hist) . . (+327) . . N 20.109(S23):M2D7 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}} ==Introduction== ==Navigation links== Next day: 20.109(S23):M2D8 |IF stain...")
- 22:16, 31 January 2023 (diff | hist) . . (+228) . . 20.109(S23):M2D8
- 22:14, 31 January 2023 (diff | hist) . . (+97) . . 20.109(S23):M2D8
- 22:14, 31 January 2023 (diff | hist) . . (+1,658) . . N 20.109(S23):M2D8 (Created page with "===Organize figures and outline results text for your Research article=== The goal for today is to focus on how you will communicate the results you are gathering and analyzi...")
- 22:02, 30 January 2023 (diff | hist) . . (-13) . . 20.109(S23):Spring 2023 schedule
- 16:48, 30 January 2023 (diff | hist) . . (+208) . . 20.109(S23):Spring 2023 schedule
- 16:47, 30 January 2023 (diff | hist) . . (+52) . . 20.109(S23):Assignments (→Contribution to Class blog) (current)
- 19:40, 26 January 2023 (diff | hist) . . (+6) . . 20.109(S23):Spring 2023 schedule
- 17:31, 26 January 2023 (diff | hist) . . (+1) . . 20.109(S23):Spring 2023 schedule
- 19:29, 17 January 2023 (diff | hist) . . (+11,838) . . N 20.109(S23):M2D4 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}} ==Introduction== Image:Fa16 M2D7 tet repression schematic.png|thumb|right|5...")
- 19:25, 17 January 2023 (diff | hist) . . (+15,097) . . N 20.109(S23):M2D3 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}} ==Introduction== The CRISPRi system involves three genetic components: the pd...")
- 19:21, 17 January 2023 (diff | hist) . . (+10,393) . . 20.109(S23):M2D2
- 19:20, 17 January 2023 (diff | hist) . . (+16,051) . . 20.109(S23):M2D1
- 19:18, 17 January 2023 (diff | hist) . . (+116) . . N 20.109(S23):M2D2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}} Welcome to 20.109!")
- 19:17, 17 January 2023 (diff | hist) . . (+21) . . 20.109(S23):M2D1
- 19:17, 17 January 2023 (diff | hist) . . (+95) . . N 20.109(S23):M2D1 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S23)}}")
- 18:53, 17 January 2023 (diff | hist) . . (+1) . . 20.109(S23):Spring 2023 schedule
- 18:52, 17 January 2023 (diff | hist) . . (+2) . . 20.109(S23):Spring 2023 schedule
- 18:51, 17 January 2023 (diff | hist) . . (+6) . . 20.109(S23):Spring 2023 schedule
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