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|style="width: 35%;"|'''Resources''' | |style="width: 35%;"|'''Resources''' | ||
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− | + | | Week 9: Oct. 30 - Nov. 5 | |
− | | | + | | Dive into circuits with the electronics bootcamp |
− | + | | <font color=#04B45F> Friday Nov. 4th: Electronics Bootcamp</font color> | |
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| [[Electronics Mini-Lab|Electronics bootcamp]] | | [[Electronics Mini-Lab|Electronics bootcamp]] | ||
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* [[Complex Number Review|Complex number review]] | * [[Complex Number Review|Complex number review]] | ||
* [[Input and output impedance]] | * [[Input and output impedance]] | ||
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− | | Week | + | | Week 10: Nov. 6 - Nov. 12 |
− | | | + | | Start DNA Melting Part 1 |
− | | <font color=#04B45F> | + | | <font color=#04B45F> Tuesday Nov. 8th: Quiz 2 (during lecture)</font color> |
− | | [[ | + | | [[DNA Melting Part 1: Measuring Temperature and Fluorescence]] |
− | | | + | [[DNA Melting Report Requirements for Part 1]] |
− | |} | + | | Overview |
+ | * [[Lab Manual:Measuring DNA Melting Curves]] | ||
+ | Code examples and simulations | ||
+ | * [[DNA Melting: Simulating DNA Melting - Basics]] | ||
+ | * [[DNA Melting: Simulating DNA Melting - Intermediate Topics]] | ||
+ | * [[DNA Melting: Model function and parameter estimation by nonlinear regression]] | ||
+ | Reference materials | ||
+ | * [[DNA Melting Thermodynamics]] | ||
+ | * [[DNA Melting: DNA Sequences]] | ||
+ | * [http://mfold.rna.albany.edu/?q=DINAMelt/Hybrid2 DINAMelt Web Server] | ||
+ | Background reading | ||
+ | * [[Electronics Primer]] | ||
+ | <!-- * [[Real electronics]] -->|} | ||
</div> | </div> | ||
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20.309 This week and next in the 20.309 lab
Week | Summary | Assignments due | Lab Manual | Resources |
Week 9: Oct. 30 - Nov. 5 | Dive into circuits with the electronics bootcamp | Friday Nov. 4th: Electronics Bootcamp | Electronics bootcamp | |
Week 10: Nov. 6 - Nov. 12 | Start DNA Melting Part 1 | Tuesday Nov. 8th: Quiz 2 (during lecture) | DNA Melting Part 1: Measuring Temperature and Fluorescence | Overview
Code examples and simulations
Reference materials Background reading |
Contents
20.309 course information
Reserve a lab station here: Bioinstrumentation Lab reservation system
Optics bootcamp
Lab manual
Background reading
- Geometrical optics and ray tracing
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
Optical microscopy lab
Code examples and simulations
- Converting Gaussian fit to Rayleigh resolution
- MATLAB: Estimating resolution from a PSF slide image
- Matlab: Scalebars
- Calculating MSD and Diffusion Coefficients
Background reading
- Geometrical optics and ray tracing
- Physical optics and resolution
- Optical aberrations
- Aperture and field stops
- Optical detectors, noise, and the limit of detection
- Manta G032 camera measurements
- Understanding log plots
Electronics bootcamp
Lab manual
Background reading
- Electronics primer
- Khan Academy Material, esp. "Ideal Circuit Elements" and "Voltage Divider"
- Real electronics
- Impedance analysis
- Transfer functions and Bode plots
- Complex number review
- Input and output impedance
DNA melting lab
Lab manuals
- Lab Manual:Measuring DNA Melting Curves
- DNA Melting Part 1: Measuring Temperature and Fluorescence
- DNA Melting Report Requirements for Part 1
- DNA Melting: Model function and parameter estimation by nonlinear regression
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA melting: Identifying the unknown sample
- DNA Melting Part 2: Lock-in Amplifier and Temperature Control
- DNA Melting Report Requirements for Part 2
Code examples and simulations
- DNA Melting: Simulating DNA Melting - Basics
- DNA Melting: Simulating DNA Melting - Intermediate Topics
- DNA Melting: Model function and parameter estimation by nonlinear regression
Reference materials
Background reading
Limits of detection mini-lab
- Lab Manual: Limits of Detection
- Lab Manual: Atomic Force Microscopy (AFM)
- Limits of Detection: Data Sessions