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• Spring 2012:Vincent Lee Journal
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  8 KB (1,373 words) - 18:43, 13 August 2013
• Spring 2012: Jonathan Gootenberg Journal
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  11 KB (1,937 words) - 22:57, 22 May 2012
• 20.345:Key Factors Table
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  2 KB (306 words) - 01:05, 21 March 2012
• Spring 2012:LFM
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  4 KB (576 words) - 05:19, 18 May 2012
• Understanding the lock-in amplifier
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  15 KB (1,965 words) - 16:36, 13 November 2017
• Optical Microscopy Part 4: Particle Tracking
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  9 KB (1,469 words) - 01:01, 10 March 2016
• Protocols for cell culture
  * Water at the bottom: * Blower always on, vented at the bottom (20.109 students, beware: this type of hood is different from regular ones
  18 KB (2,896 words) - 18:17, 25 February 2024
• Protocols
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  4 KB (644 words) - 18:24, 15 August 2013
• Optical Microscopy Part 1: Brightfield Microscopy
  .... Also, note that the stages are very expensive; always lift them from the bottom. ... Week 1 report: (top) Air Force target, (center) Silica spheres and dust, (bottom) Ronchi Ruling]]
  23 KB (3,758 words) - 22:45, 12 January 2017
• Aligning the optical trap
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  4 KB (645 words) - 22:09, 3 May 2013
• Optical Microscopy Part 2: Fluorescence Microscopy
  ...he day prior to the fluorescence imaging, cells were plated on 35 mm glass-bottom cell culture dishes (MatTek, equipped with coverslip suited for optical mic ...ple images and histogram. Top: fluorescent beads; center: reference slide, bottom: image histogram.]]
  20 KB (3,262 words) - 22:06, 14 August 2017
• Optical detectors, noise, and the limit of detection
  ===Bottom line on optical detector noise=== {{Template:20.309 bottom}}
  14 KB (2,102 words) - 13:42, 3 March 2021
• Error analysis
  ...ial configuration to be as insensitive as possible to such effects" </ref> Bottom line: systematic errors are much harder to characterize than random errors. ...g the subject of measurement error seem more mysterious than it really is. Bottom line: Precision quantifies the variability of a measurement. Accuracy speci
  24 KB (3,757 words) - 14:57, 3 September 2018
• Optics Bootcamp
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  28 KB (4,857 words) - 20:09, 31 July 2017
• 20.109(S08):Testing cell viability (Day3)
  ... over (to the right). This is to exclude any cells that are growing on the bottom of the plate (as opposed to actually in the beads) from analysis. #Turn on the illumination using the button at the bottom left of the microscope body (on the right-hand side is a light intensity sl
  18 KB (3,087 words) - 16:04, 15 June 2015
• 20.109(S08):Initiate cell culture (Day2)
  ...n body inside this sterile area. (By back wall I mean what will become the bottom inside wall when you lay the flask down, which is the surface on which your
  12 KB (2,006 words) - 16:04, 15 June 2015
• 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)
  ... manually adjust the margins of your document so they are 0.6 inches (top, bottom, left and right) and you should change the text to 10 point Courier font. C ...ll be assembling the components of the “D32N-fwd primer 5’- ” at the bottom of your MSWord document. There are several things to consider as you design
  27 KB (3,752 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Clean and cut DNA (Day 2)
  ...g and palindromic, that is, they read the same 5’ to 3’ on the top and bottom strand of DNA. For example, the recognition sequence for EcoRI (see also fi ... for one minute and then spin as before. The material that collects in the bottom of the eppendorf tube is your PCR product, ready to be digested.
  10 KB (1,692 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/Agarose gel electrophoresis (Day 3)
  ...quick spin them in the microfuge to bring the contents of the tubes to the bottom. ... surface of the buffer and directly over the well. You risk puncturing the bottom of the well if you lower the tip too far into the well itself (puncturing w
  10 KB (1,806 words) - 14:24, 5 June 2015
• 20.109(S08):DNA engineering/DNA ligation and bacterial transformation (Day 4)
  ...in each of the eppendorf tubes. They may appear as solid white dots at the bottom corner of the tube or they may appear to be a diffuse white smear along the ...vortex and pipet up and down several times. Bring any droplets down to the bottom of the tubes with a quick spin in the microfuge.
  10 KB (1,704 words) - 14:24, 5 June 2015