20.109(S17):Purification of induced protein (Day2)

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20.109(S17): Laboratory Fundamentals of Biological Engineering

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Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

Introduction

Protocols

Part 1: Lyse BL21(DE3)pLysS pRSETb_FKBP12 cells

Part 2: Purify FKBP12 protein

Reagents

Navigation links

Next day: Evaluation of purified protein

Previous day: In silico cloning and induction of protein expression
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