20.109(S17):Module 1

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20.109(S17): Laboratory Fundamentals of Biological Engineering


Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

Module 1

Lecturer: Angela Koehler
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas

TA: Rob Wilson
Lab manager: Hsinhwa Lee


Chemical probes are important research tools used to explore cellular processes and therapeutic targets. The use of high-throughput and unbiased strategies to identify small molecules that bind specific biomolecules, such as proteins, can provide insight on the structure or function of targets. Additionally, a small-molecule screen can identify new chemical probes for target proteins of interest.

The small-molecule microarray (SMM) is a high-throughput method that enables the detection of protein-ligand binding. Briefly, ligands are 'printed' onto a slide and incubated with purifed protein. Unbound protein is washed from the slide and bound protein is detected using a tag on the protein of interest. Because the location of every ligand on the slide is known, the detection of protein indicates that it is bound to the ligand at that location.

In your experiment, you will use SMMs to identify ligands that bind to FKBP12, a folding chaperone for proteins that contain proline residues in eukaryotes.

This module has been developed thanks to the generous time and thoughtful efforts of several Koehler Laboratory members, in particular Rob Wilson, Dr. Becky Leifer, and Shelby Doyle.

Sp17 20.109 M1 overview.png

Lab links: day by day

M1D1: In silico cloning and induce protein expression
M1D2: Purify induced protein
M1D3: Evaluate purity and concentration of protein
M1D4: Screen ligand library for FKBP12 binders
M1D5: Scan slides to identify FKBP12 binders
M1D6: Complete data analysis
M1D7: Identify chemical structures common in FKBP12 binders


See all M1 student data on the Discussion page.


Data summary


Notes for teaching faculty

S17 notes for M1

S17 notes for orientation day