20.109(S17):Module 2

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20.109(S17): Laboratory Fundamentals of Biological Engineering

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Schedule Spring 2017        Announcements        Assignments        Homework        Communication
       1. High-throughput ligand screening        2. Gene expression engineering        3. Biomaterials engineering              

Module 2

Lecturers: Leona Samson and Ernest Fraenkel
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas

TA: Rob Wilson
Lab manager: Hsinhwa Lee

Overview

Sp17 20.109 M2 overview.png


Lab links: day by day

M2D1: Confirm cell lines and practice tissue culture techniques
M2D2: Complete Western blot and induce DNA damage for survival and quantitative PCR assays
M2D3: Assess cell survival and harvest RNA for quantitative PCR assay
M2D4: Examine transcript levels in response to DNA damage
M2D5: Journal club I
M2D6: Practice data analysis methods
M2D7: Analyze RNA-seq data
M2D8: Journal club II
M2D9: Examine quantitative PCR results

Data

See all M2 student data on the Discussion page.

Assignments

Journal club presentation
Research article

References

Notes for teaching faculty

S17 notes for M2
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