Difference between revisions of "20.109(S17):Module 1"
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==Lab links: day by day== | ==Lab links: day by day== | ||
| − | [[20.109(S17):In silico cloning and induction of protein expression (Day1) |In silico cloning and induce protein expression]]<br> | + | M1D1: [[20.109(S17):In silico cloning and induction of protein expression (Day1) |In silico cloning and induce protein expression]]<br> |
| − | [[20.109(S17):Purification of induced protein (Day2) |Purify induced protein]]<br> | + | M1D2: [[20.109(S17):Purification of induced protein (Day2) |Purify induced protein]]<br> |
| − | [[20.109(S17):Evaluation of purified protein (Day3) |Evaluate purity and concentration of protein]]<br> | + | M1D3: [[20.109(S17):Evaluation of purified protein (Day3) |Evaluate purity and concentration of protein]]<br> |
| − | [[20.109(S17):Screen chemical library for FKBP12 binders (Day4) |Screen ligand library for FKBP12 binders]]<br> | + | M1D4: [[20.109(S17):Screen chemical library for FKBP12 binders (Day4) |Screen ligand library for FKBP12 binders]]<br> |
| − | [[20.109(S17):Scan slides to identify FKBP12 binders (Day5) |Scan slides to identify FKBP12 binders]]<br> | + | M1D5: [[20.109(S17):Scan slides to identify FKBP12 binders (Day5) |Scan slides to identify FKBP12 binders]]<br> |
| − | [[20.109(S17):Complete data analysis (Day6) |Complete data analysis]]<br> | + | M1D6: [[20.109(S17):Complete data analysis (Day6) |Complete data analysis]]<br> |
| − | [[20.109(S17):Identify chemical structures common among FKBP12 binders |Identify chemical structures common among FKBP12 binders]]<br> | + | M1D7: [[20.109(S17):Identify chemical structures common among FKBP12 binders |Identify chemical structures common among FKBP12 binders]]<br> |
==Data== | ==Data== | ||
Revision as of 22:48, 30 January 2017
Contents
Module 1
Lecturer: Angela Koehler
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA: Rob Wilson
Lab manager: Hsinhwa Lee
Overview
Lab links: day by day
M1D1: In silico cloning and induce protein expression
M1D2: Purify induced protein
M1D3: Evaluate purity and concentration of protein
M1D4: Screen ligand library for FKBP12 binders
M1D5: Scan slides to identify FKBP12 binders
M1D6: Complete data analysis
M1D7: Identify chemical structures common among FKBP12 binders
Data
See all M1 student data on the Discussion page.
Assignments
Data summary
Mini-presentation
References
A method for the covalent capture and screening of diverse small molecules in a microarray format. Nature Protocols. 1:2344-2352.
Recent discoveries and applications involving small-molecule microarrays. Chemical Biology. 18:21-28.
