Difference between revisions of "20.109(S17):Module 1"
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[[20.109(S17):In silico cloning and induction of protein expression (Day1) |In silico cloning and induce protein expression]]<br> | [[20.109(S17):In silico cloning and induction of protein expression (Day1) |In silico cloning and induce protein expression]]<br> | ||
[[20.109(S17):Purification of induced protein (Day2) |Purify induced protein]]<br> | [[20.109(S17):Purification of induced protein (Day2) |Purify induced protein]]<br> | ||
| − | [[20.109(S17):Evaluation of purified protein (Day3) |Evaluate purity and concentration of protein | + | [[20.109(S17):Evaluation of purified protein (Day3) |Evaluate purity and concentration of protein]]<br> |
[[20.109(S17):Screen chemical library for FKBP12 binders (Day4) |Screen ligand library for FKBP12 binders]]<br> | [[20.109(S17):Screen chemical library for FKBP12 binders (Day4) |Screen ligand library for FKBP12 binders]]<br> | ||
[[20.109(S17):Scan slides to identify FKBP12 binders (Day5) |Scan slides to identify FKBP12 binders]]<br> | [[20.109(S17):Scan slides to identify FKBP12 binders (Day5) |Scan slides to identify FKBP12 binders]]<br> | ||
Revision as of 22:46, 30 January 2017
Contents
Module 1
Lecturer: Angela Koehler
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA: Rob Wilson
Lab manager: Hsinhwa Lee
Overview
Lab links: day by day
In silico cloning and induce protein expression
Purify induced protein
Evaluate purity and concentration of protein
Screen ligand library for FKBP12 binders
Scan slides to identify FKBP12 binders
Complete data analysis
Identify chemical structures common among FKBP12 binders
Data
See all M1 student data on the Discussion page.
Assignments
Data summary
Mini-presentation
References
A method for the covalent capture and screening of diverse small molecules in a microarray format. Nature Protocols. 1:2344-2352.
Recent discoveries and applications involving small-molecule microarrays. Chemical Biology. 18:21-28.
