Light Field Microscopy

First Proposal

Here is the link to our proposal.

Literature Search

Light field imaging allows for the capture of the light field of a sample in a single photograph.^[1] This has been recently commercialized in the Lytro Camera^[2] which obtains a light field using a microlens and allows for processing of the image afterwards.

They also documented their setup in more detail in a technical memo.^[4]

Introduction to LFDisplay.^[5]

Light field rendering.^[6]

In 2009, the group published a better quality machine 4D Microscopy^[7]

Objectives

I would be thrilled to work on this project for a number of reasons:

• It will force me to work on image processing, I am novice status :p
• It would be an awesome project
• It is feasible
  • Only one additional physical component in a traditional bright field microscope
  • Lots of code
• It is relevant
  • This microscope can be used to compose a 3-dimensional image of the sample
  • Taking 3-dimensional images quickly is a current point of interest in microscopy today
• It is novel
  • With respect to this class, this is a novel project
  • Otherwise, this is not a new technology ^[6] but has been implemented into prototype microscopes in the past 7 years

Final Submission

Here is the link to our final report and documentation.

References

1. ↑ A practical introduction to light field microscopy - Stanford 2010 (pdf)
2. ↑ Light field camera
3. ↑ Light field microscopy - Stanford 2006 (pdf)
4. ↑ Optical recipes for light field microscopes - Stanford 2006 (pdf)
5. ↑ LFDisplay: A real time system for light field microscopy
6. ↑ ^{6.0 6.1} Light field rendering - Stanford 1996
7. ↑ Recording and controlling the 4D light field in a microscope - Stanford 2009 (pdf)