Difference between revisions of "Assignment 3 Overview"
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===Background reading=== | ===Background reading=== | ||
* [[Flat-field correction|Flat-field correction of non-uniform illumination]] | * [[Flat-field correction|Flat-field correction of non-uniform illumination]] | ||
− | + | * [[Geometrical optics and ray tracing]] | |
+ | * [[Physical optics and resolution]] | ||
+ | * [[Optical aberrations]] | ||
+ | * [[Aperture and field stops]] | ||
+ | * [[Optical detectors, noise, and the limit of detection]] | ||
+ | * [[Manta G032 camera measurements]] | ||
+ | * [[Understanding log plots]] | ||
{{Template:20.309 bottom}} | {{Template:20.309 bottom}} |
Revision as of 20:56, 14 August 2017
Assignment details
This assignment has 2 parts:
- Part 1: Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-field correction code you wrote for Assignment 2 to address non-uniform illumination;
- Part 2: Explore interesting calculations and considerations to guide your experimental design with fluorescence;
Turn in all of your work on Stellar in a single PDF file named <lastname><firstname>Assignment3.pdf.