Difference between revisions of "Assignment 3 Overview"
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# [[Assignment 3, Part 2: experimental design with fluorescence| Part 2:]] Explore interesting calculations and considerations to guide your experimental design with fluorescence. | # [[Assignment 3, Part 2: experimental design with fluorescence| Part 2:]] Explore interesting calculations and considerations to guide your experimental design with fluorescence. | ||
− | + | Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment3.pdf. Here is a checklist of all things you have to turn in: | |
− | Turn in: | + | |
+ | {{Template:Assignment Turn In|message= Here is a comprehensive list of what you need to turn in: | ||
+ | |||
# A figure with images of the 3.26 μm fluorescent microsphere samples, and the stained cell samples with and without Cyto-D. | # A figure with images of the 3.26 μm fluorescent microsphere samples, and the stained cell samples with and without Cyto-D. | ||
#* For each sample, create 1 figure with 5 panels. | #* For each sample, create 1 figure with 5 panels. | ||
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#* What differences did you observe between the cells with and without CytoD? | #* What differences did you observe between the cells with and without CytoD? | ||
# Answers to all questions in [[Assignment 3, Part 2: experimental design with fluorescence|Assignment 3, Part 2]]. | # Answers to all questions in [[Assignment 3, Part 2: experimental design with fluorescence|Assignment 3, Part 2]]. | ||
+ | }} | ||
+ | {{Template:Assignment 3 navigation}} | ||
− | + | ==Background reading== | |
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* [[Flat-field correction|Flat-field correction of non-uniform illumination]] | * [[Flat-field correction|Flat-field correction of non-uniform illumination]] | ||
* [[Geometrical optics and ray tracing]] | * [[Geometrical optics and ray tracing]] |
Revision as of 22:53, 11 September 2017
Assignment details
This assignment has 2 parts:
- Part 1: Use the epifluorescence microscope you built to image fixed biological samples, and use the flat-field correction code you wrote for Assignment 2 to address non-uniform illumination;
- Part 2: Explore interesting calculations and considerations to guide your experimental design with fluorescence.
Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment3.pdf. Here is a checklist of all things you have to turn in:
Here is a comprehensive list of what you need to turn in:
|
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