Difference between revisions of "20.109(F08): Fall 2008 schedule"

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|  
 
|  
 
| Thur Sept 4  
 
| Thur Sept 4  
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| [[Natalie Kuldell | NK]]
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| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|
+
−
| [[20.109(F07):Lab tour | Orientation]]
+
 
|  
 
|  
 +
| [[20.109(F08):Lab tour | Orientation]]
 +
| [[Media:20.109(F08) OrientationHandout.pdf| orientation day handout]]
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
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| Tue Sept 9  
 
| Tue Sept 9  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
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| [[20.109(F07):Start-up genome engineering | Start-up genome engineering]]
+
| [[20.109(F08): Mod 1 Day 1 DNA engineering using PCR | DNA engineering using PCR]]
−
|  
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| [[Media:MOD1DAY1.pdf| NK lab notes]], [[Media:F08_M1D1-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
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| Thur Sept 11   
 
| Thur Sept 11   
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Agarose gel electrophoresis | Agarose gel electrophoresis]]
+
| [[20.109(F08): Mod 1 Day 2 Clean and cut DNA | Clean and cut DNA]]
−
|  
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| [[Media:MOD1DAY2.pdf| NK lab notes]], [[Media:F08_M1D2-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
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| Tue Sept 16  
 
| Tue Sept 16  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): DNA ligation and bacterial transformation| DNA ligation and bacterial transformation]]
+
| [[20.109(F08): Mod 1 Day 3 Agarose gel electrophoresis| Agarose gel electrophoresis]]
−
|  
+
| [[Media:MOD1DAY3.pdf| NK lab notes]], [[Media:F08_M1D3-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
 
| 4
 
| 4
 
| Thur Sept 18  
 
| Thur Sept 18  
−
| [[http://web.mit.edu/be/people/engelward.htm BE]
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| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Examine candidate clones| Examine candidate clones]]
+
| [[20.109(F08): Mod 1 Day 4 DNA ligations and bacterial transformations| DNA ligation and bacterial transformation]]
−
|  
+
| [[Media:MOD1DAY4.pdf| NK lab notes]], [[Media:F08_M1D4-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
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| Tue Sept 23  
 
| Tue Sept 23  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): M13.1| M13.1]]
+
| [[20.109(F08): Mod 1 Day 5 Examine candidate clones| Examine candidate clones]]
−
|  
+
| [[Media:MOD1DAY5.pdf| NK lab notes]], [[Media:F08_M1D5-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
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| Thur Sept 25  
 
| Thur Sept 25  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Western analysis| Western analysis]]
+
| [[20.109(F08): Mod 1 Day 6 Restriction map and tissue culture | Restriction map and tissue culture]]
−
|  
+
| [[Media:Mod1day6.ppt| NK lab notes]], [[Media:F08_M1D6-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
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| Tue Sept 30  
 
| Tue Sept 30  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Probe western| Probe western]]
+
| [[20.109(F08): Mod 1 Day 7 Lipofection| Lipofection]]
−
|  
+
| your first draft of the [[20.109(F08):DNA engineering lab report guidelines| Mod 1 lab report]] is due before lab today<br>
 +
[[Media:F08_M1D7-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 1
 
| 1
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| Thur Oct 2  
 
| Thur Oct 2  
 
| [http://web.mit.edu/be/people/engelward.htm BE]
 
| [http://web.mit.edu/be/people/engelward.htm BE]
−
|  
+
| [http://openwetware.org/wiki/User:Michelle_R._Sukup MS]
−
| [[20.109(F07): Module 1 oral presentations| Oral Presentations]]
+
| [[20.109(F08): Mod 1 Day 8 FACS analysis| FACS analysis]]
 
|  
 
|  
 
|--
 
|--
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| 1
 
| 1
 
| Tues Oct 7
 
| Tues Oct 7
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): siRNA design| siRNA design and start-up in cell culture]]
+
| [[20.109(F08): Mod 2 Day 1 Protein engineering with PCR| Protein engineering with PCR]]
−
|  
+
| your [[20.109(F08):DNA engineering powerpoint pitch guidelines| PowerPoint Pitch]] is due before lab today<br>[[Media:20.109(F08) handoutM2D1.pdf| M2D1 lecture notes]]<br>[[Media:MOD2DAY1_wiki.ppt.pptx| NK lab notes]], [[Media:F08_M2D1-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 2
 
| 2
 
| 2
 
| 2
 
| Thur Oct 9
 
| Thur Oct 9
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Transfection| Transfection]]
+
| [[20.109(F08): Mod 2 Day 2 Yeast transformation| Yeast transformation]]
−
|  
+
| [[Media:20.109(F08) handout M2D2.pdf| M2D2 lecture notes]]<br>[[Media:MOD2DAY2_wiki.ppt| NK lab notes]], [[Media:F08_M2D2-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 2
 
| 2
 
| 3
 
| 3
 
| Tues Oct 14
 
| Tues Oct 14
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Luciferase assays and RNA prep| Luciferase assays and RNA prep]]
+
| [[20.109(F08): Mod 2 Day 3 Colony PCR and journal article discussion| Colony PCR, Journal article discussion]]
−
|
+
| [[Media:20.109(F08) handout M2D3.pdf| M2D3 lecture notes]]<br>[[Media:MOD2DAY3_wiki.ppt| NK lab notes]], [[Media:F08_M2D3-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 2
 
| 2
 
| 4
 
| 4
 
| Thur Oct 16
 
| Thur Oct 16
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Journal article discussion| Journal article discussion]]
+
| [[20.109(F08): Mod 2 Day 4 SDS-PAGE, screen for phenotypes| SDS-PAGE, screen for phenotypes]]
−
| Guest lecture: [[User:Nlerner| NL]] from the writing program.  
+
| [[Media:20.109(F08) handout M2D4.pdf| M2D4 lecture notes]] <br>[[Media:MOD2DAY4_wiki.ppt| NK lab notes]], [[Media:F08_M2D4-ANS.pdf| ANS lab notes]]
 +
 
 
|-
 
|-
 
| 2
 
| 2
 
| 5
 
| 5
 
| Tues Oct 21
 
| Tues Oct 21
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): cDNA synthesis and microarray| cDNA synthesis and microarray]]
+
| [[20.109(F08): Mod 2 Day 5 Probe western, isolate RNA| Probe Western, isolate RNA]]
−
|  
+
| guest lecture by [http://web.mit.edu/be/people/white.htm Prof. Forest White] about mass spec analysis of protein complexes. His slides are [[Media:20 109 MS lecture.ppt| here]]<br>[[Media:MOD2DAY5_wiki.ppt| NK lab notes]], [[Media:F08_M2D5-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 2
 
| 2
 
| 6
 
| 6
 
| Thurs Oct 23
 
| Thurs Oct 23
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Microarray data analysis| Microarray data analysis]]
+
| [[20.109(F08): Mod 2 Day 6 Journal Club I| Journal Club I]]
−
|  
+
| optional visit to [http://www.abcam.com/ Abcam] during lecture hour
 
|--
 
|--
 
| 2
 
| 2
 
| 7
 
| 7
 
| Tues Oct 28  
 
| Tues Oct 28  
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| Lecture on high throughput technologies;[[20.109(F07): Mod 2 Day 7| '''no lab''']]
+
| [[20.109(F08): Mod 2 Day 7 cDNA synthesis and microarray| cDNA synthesis and microarray]]
−
|  
+
| [[Media:20.109(F08) M2D7 handout.pdf| M2D7 lecture notes]]<br>[[Media:MOD2DAY7_wiki.ppt| NK lab notes]], [[Media:F08_M2D7-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 2
 
| 2
 
| 8
 
| 8
 
| Thurs Oct 30   
 
| Thurs Oct 30   
−
| [[Natalie Kuldell | NK]]
+
| [http://openwetware.org/wiki/Natalie_Kuldell  | NK]
−
|  
+
| [http://openwetware.org/wiki/User:Brian_Belmont BB]
−
| [[20.109(F07): Module 2 oral presentations| Oral presentations]]
+
| [[20.109(F08): Mod 2 Day 8 Microarray data analysis| Microarray data analysis]]
 
|  
 
|  
 
|--
 
|--
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| 1
 
| 1
 
| Tues Nov 4
 
| Tues Nov 4
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F07): Growth of phage materials| Growth of phage materials]]
+
| [[20.109(F08): Mod 3 Day 1 Growth of phage materials| Phage growth for materials]]
−
|  
+
| your first draft of your research article is due before lab today<br>[[Media:Mod3Day1.ppt| NK lab notes]], [[Media:F08_M3D1-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 3
 
| 3
 
| 2
 
| 2
−
| Tue Nov 6  
+
| Thurs Nov 6  
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F07): Phage nanowires| Phage nanowires]]
+
| [[20.109(F08): Mod 3 Day 2 Phage nanowires| Phage nanowires]]
−
|  
+
| [[Media:Mod3Day2.ppt| NK lab notes]], [[Media:F08_M3D2-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
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| 3
 
| 3
 
| Thur Nov 13
 
| Thur Nov 13
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F07): Transmission electron microscopy| TEM]]
+
| [[20.109(F08): Mod 3 Day 3 Journal Club II| Journal Club II]]
 
|  
 
|  
 
|--
 
|--
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| 4
 
| 4
 
| Tue Nov 18   
 
| Tue Nov 18   
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109 (F07): Phage by design| Phage by design]]
+
| [[20.109(F08): Mod 3 Day 4 Phage by design| Phage by design]]
−
|  
+
| [[Media:Mod3Day4.ppt| NK lab notes]], [[Media:F08_M3D4-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 3
 
| 3
 
| 5
 
| 5
 
| Thur Nov 20
 
| Thur Nov 20
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F07): Phage by design, pt2| Phage by design, pt 2]]
+
| [[20.109(F08): Mod 3 Day 5 Phage by design, pt2| Phage by design, pt 2]]
−
|  
+
| [[Media:Mod3Day5.ppt| NK lab notes]], [[Media:F08_M3D4-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
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| 6
 
| 6
 
| Tue Dec 2  
 
| Tue Dec 2  
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|  
+
| [http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F07): ECD assembly| ECD assembly]]
+
| [[20.109(F08): Mod 3 Day 6 ECD assembly| ECD assembly]]
−
|  
+
| [[Media:Mod3Day6.ppt| NK lab notes]], [[Media:F08_M3D6-ANS.pdf| ANS lab notes]]
 
|--
 
|--
 
| 3
 
| 3
 
| 7
 
| 7
 
| Thur Dec 4  
 
| Thur Dec 4  
−
| [[User:Abelcher| AB]]
+
| [http://openwetware.org/wiki/User:Abelcher | AB]
−
|
+
|[http://openwetware.org/wiki/Luis_Alvarez LA]
−
| [[20.109(F07): Module 3 oral presentations| Oral presentations]]
+
| [[20.109(F08): Mod 3 Day 7 Oral presentations| Oral presentations]]
 
|  
 
|  
 
|--
 
|--
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| 8
 
| 8
 
| Tue Dec 9
 
| Tue Dec 9
−
| [[User:Abelcher| AB]]
+
|  
 
|  
 
|  
 
| end of term celebration...
 
| end of term celebration...
−
| Lecture, no lab, for class evaluations then lunch from noon-1:30 PM,  
+
| Lecture for class evaluations then lunch from noon-1:30 PM, no lab
 
|}
 
|}
 
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Latest revision as of 19:05, 28 July 2015


20.109(F08): Laboratory Fundamentals of Biological Engineering

20.109(F08)pic.jpg

Home        People        Schedule Fall 2008        Assignments        Lab Basics        OWW Basics       
DNA Engineering        Protein Engineering        Biomaterials Engineering              

MODULE DAY DATE LECTURER TA LAB note
Thur Sept 4 NK] Orientation orientation day handout
1 1 Tue Sept 9 BE MS DNA engineering using PCR NK lab notes, ANS lab notes
1 2 Thur Sept 11 BE MS Clean and cut DNA NK lab notes, ANS lab notes
1 3 Tue Sept 16 BE MS Agarose gel electrophoresis NK lab notes, ANS lab notes
1 4 Thur Sept 18 BE MS DNA ligation and bacterial transformation NK lab notes, ANS lab notes
1 5 Tue Sept 23 BE MS Examine candidate clones NK lab notes, ANS lab notes
1 6 Thur Sept 25 BE MS Restriction map and tissue culture NK lab notes, ANS lab notes
1 7 Tue Sept 30 BE MS Lipofection your first draft of the Mod 1 lab report is due before lab today

ANS lab notes

1 8 Thur Oct 2 BE MS FACS analysis
2 1 Tues Oct 7 NK] BB Protein engineering with PCR your PowerPoint Pitch is due before lab today
M2D1 lecture notes
NK lab notes, ANS lab notes
2 2 Thur Oct 9 NK] BB Yeast transformation M2D2 lecture notes
NK lab notes, ANS lab notes
2 3 Tues Oct 14 NK] BB Colony PCR, Journal article discussion M2D3 lecture notes
NK lab notes, ANS lab notes
2 4 Thur Oct 16 NK] BB SDS-PAGE, screen for phenotypes M2D4 lecture notes
NK lab notes, ANS lab notes
2 5 Tues Oct 21 NK] BB Probe Western, isolate RNA guest lecture by Prof. Forest White about mass spec analysis of protein complexes. His slides are here
NK lab notes, ANS lab notes
2 6 Thurs Oct 23 NK] BB Journal Club I optional visit to Abcam during lecture hour
2 7 Tues Oct 28 NK] BB cDNA synthesis and microarray M2D7 lecture notes
NK lab notes, ANS lab notes
2 8 Thurs Oct 30 NK] BB Microarray data analysis
3 1 Tues Nov 4 AB] LA Phage growth for materials your first draft of your research article is due before lab today
NK lab notes, ANS lab notes
3 2 Thurs Nov 6 AB] LA Phage nanowires NK lab notes, ANS lab notes
Veteran's Day Break Nov 10,11 No lab Tuesday and Wednesday
3 3 Thur Nov 13 AB] LA Journal Club II
3 4 Tue Nov 18 AB] LA Phage by design NK lab notes, ANS lab notes
3 5 Thur Nov 20 AB] LA Phage by design, pt 2 NK lab notes, ANS lab notes
Thanksgiving Break Nov No lab or lecture all week
3 6 Tue Dec 2 AB] LA ECD assembly NK lab notes, ANS lab notes
3 7 Thur Dec 4 AB] LA Oral presentations
3 8 Tue Dec 9 end of term celebration... Lecture for class evaluations then lunch from noon-1:30 PM, no lab
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