Talk:20.109(S16):Design mutation primers (Day2)
From Course Wiki
Primers for SDM
| Team | Forward primer | Reverse primer | Mutation (X#Z in CaM) | |
| TR red | 5' CACCACAAAGagACTTGGCACCGTTATG 3' | 5' ATGGTGCCGTCCCCATCC 3' | E30R | |
| TR orange | 5' ATTCGACAAGcatGGGGACGGCA 3' | 5' AATGAGAAGGCTTCTTTGAACTCTG 3' | D21H | |
| TR yellow | CACCACAAAGaAACTTGGCACCG | ATGGTGCCGTCCCCATCC | E30K | |
| TR green | 5' TTCGACAAGGgTGGGGACGGC 3' | 5' TAATGAGAAGGCTTCTTTGAACTCTGC 3' | D62G | |
| TR blue | 5' CAAGGATGGGaACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23N | |
| TR pink | 5' GTT GAT GAA ATG ATA AGG GAA cCA GAT ATC GAT GGT GAT GGC C 3' | 5' TTC TTC ATC TGT TAA CTT CTC CCC GAG GTT TGT CAT 3' | A127P | |
| TR purple | 5' TTTTGACAAGcATGGGAACGGC 3' | 5' ACACGGAATGCTTCTCGG 3' | D94H | |
| WF red | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23H | |
| WF orange | 5'CCGT GTT TTT GAC gAG GAT GGG AAC 3' | 5' AAT GCT TCT CGG ATT TCC TCT TCG C 3' | K93E | |
| WF blue | 5' CAATGGAACGgaTTACTTTCCTGAATTTC 3' | 5' CCATCAGCATCGACTTCATTG 3' | I62D | |
| WF pink | 5' AAT GAA GTC GCT GCT GAT GGC 3' | 5' GAT CAT ATC CTG CAA TTC TG 3' | D55A | |
| WF purple | 5' CAAGGATGGGcACGGCACCAT 3' | 5' TCGAATAATGAGAAGGCTTCTTTG 3' | D23H |
