Difference between revisions of "Talk:20.109(S16):Design mutation primers (Day2)"
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(→Primers for SDM) |
MAXINE JONAS (Talk | contribs) (→Primers for SDM) |
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|'''Forward primer''' | |'''Forward primer''' | ||
|'''Reverse primer''' | |'''Reverse primer''' | ||
| + | |'''Mutation''' | ||
|- | |- | ||
|<font color = #FF0000>TR red</font color> | |<font color = #FF0000>TR red</font color> | ||
|5' CACCACAAAGagACTTGGCACCGTTATG 3' | |5' CACCACAAAGagACTTGGCACCGTTATG 3' | ||
|5' ATGGTGCCGTCCCCATCC 3' | |5' ATGGTGCCGTCCCCATCC 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #FF9900>TR orange</font color> | |<font color = #FF9900>TR orange</font color> | ||
|5' ATTCGACAAGcatGGGGACGGCA 3' | |5' ATTCGACAAGcatGGGGACGGCA 3' | ||
|5' AATGAGAAGGCTTCTTTGAACTCTG 3' | |5' AATGAGAAGGCTTCTTTGAACTCTG 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #FFFF00>TR yellow</font color> | |<font color = #FFFF00>TR yellow</font color> | ||
|CACCACAAAGaAACTTGGCACCG | |CACCACAAAGaAACTTGGCACCG | ||
|ATGGTGCCGTCCCCATCC | |ATGGTGCCGTCCCCATCC | ||
| + | | | ||
|- | |- | ||
|<font color = #00FF00>TR green</font color> | |<font color = #00FF00>TR green</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #0000FF>TR blue</font color> | |<font color = #0000FF>TR blue</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #FF99CC>TR pink</font color> | |<font color = #FF99CC>TR pink</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #CC00FF>TR purple</font color> | |<font color = #CC00FF>TR purple</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #FF0000>WF red</font color> | |<font color = #FF0000>WF red</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #FF9900>WF orange</font color> | |<font color = #FF9900>WF orange</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #0000FF>WF blue</font color> | |<font color = #0000FF>WF blue</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #FF99CC>WF pink</font color> | |<font color = #FF99CC>WF pink</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|- | |- | ||
|<font color = #CC00FF>WF purple</font color> | |<font color = #CC00FF>WF purple</font color> | ||
|5' insert here 3' | |5' insert here 3' | ||
|5' insert here 3' | |5' insert here 3' | ||
| + | | | ||
|} | |} | ||
Revision as of 00:14, 10 February 2016
Primers for SDM
| Team | Forward primer | Reverse primer | Mutation |
| TR red | 5' CACCACAAAGagACTTGGCACCGTTATG 3' | 5' ATGGTGCCGTCCCCATCC 3' | |
| TR orange | 5' ATTCGACAAGcatGGGGACGGCA 3' | 5' AATGAGAAGGCTTCTTTGAACTCTG 3' | |
| TR yellow | CACCACAAAGaAACTTGGCACCG | ATGGTGCCGTCCCCATCC | |
| TR green | 5' insert here 3' | 5' insert here 3' | |
| TR blue | 5' insert here 3' | 5' insert here 3' | |
| TR pink | 5' insert here 3' | 5' insert here 3' | |
| TR purple | 5' insert here 3' | 5' insert here 3' | |
| WF red | 5' insert here 3' | 5' insert here 3' | |
| WF orange | 5' insert here 3' | 5' insert here 3' | |
| WF blue | 5' insert here 3' | 5' insert here 3' | |
| WF pink | 5' insert here 3' | 5' insert here 3' | |
| WF purple | 5' insert here 3' | 5' insert here 3' |
