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- 22:15, 22 January 2021 (diff | hist) . . (-750) . . 20.109(S21):Homework (→Due M2D3)
- 22:10, 22 January 2021 (diff | hist) . . (+940) . . 20.109(S21):Homework (→Due M2D7)
- 22:10, 22 January 2021 (diff | hist) . . (+1,049) . . 20.109(S21):Homework (→Due M2D6)
- 22:09, 22 January 2021 (diff | hist) . . (+866) . . 20.109(S21):Homework (→Due M2D5)
- 22:08, 22 January 2021 (diff | hist) . . (+373) . . 20.109(S21):Homework (→Due M2D4)
- 22:06, 22 January 2021 (diff | hist) . . (+1,551) . . 20.109(S21):Homework (→Due M2D3)
- 22:04, 22 January 2021 (diff | hist) . . (+904) . . 20.109(S21):Homework (→Due M2D2)
- 21:56, 22 January 2021 (diff | hist) . . (+739) . . 20.109(S21):Homework (→Due M3D5)
- 21:55, 22 January 2021 (diff | hist) . . (+785) . . 20.109(S21):Homework (→Due M3D4)
- 21:54, 22 January 2021 (diff | hist) . . (+1,090) . . 20.109(S21):Homework (→Due M3D3)
- 21:54, 22 January 2021 (diff | hist) . . (+815) . . 20.109(S21):Homework (→Due M3D2)
- 21:53, 22 January 2021 (diff | hist) . . (+173) . . 20.109(S21):Homework (→Due M3D1)
- 21:52, 22 January 2021 (diff | hist) . . (+554) . . 20.109(S21):Homework
- 21:47, 22 January 2021 (diff | hist) . . (+377) . . 20.109(S21):Homework (→Module 2: Screening small molecules)
- 21:43, 22 January 2021 (diff | hist) . . (+188) . . 20.109(S21):Homework (→Module 2: Screening small molecules)
- 21:37, 22 January 2021 (diff | hist) . . (0) . . 20.109(S21):Homework (→Due M1D2)
- 21:36, 22 January 2021 (diff | hist) . . (+130) . . 20.109(S21):Homework (→Due M1D6)
- 21:35, 22 January 2021 (diff | hist) . . (+511) . . 20.109(S21):Homework (→Due M1D6)
- 21:34, 22 January 2021 (diff | hist) . . (+381) . . 20.109(S21):Homework (→Due M1D7)
- 21:30, 22 January 2021 (diff | hist) . . (+758) . . 20.109(S21):Homework (→Due M1D6)
- 21:28, 22 January 2021 (diff | hist) . . (-207) . . 20.109(S21):Homework (→Due M1D3)
- 21:28, 22 January 2021 (diff | hist) . . (+702) . . 20.109(S21):Homework (→Due M1D5)
- 21:26, 22 January 2021 (diff | hist) . . (+1,482) . . 20.109(S21):Homework (→Due M1D4)
- 21:21, 22 January 2021 (diff | hist) . . (+518) . . 20.109(S21):Homework (→Due M1D5)
- 21:20, 22 January 2021 (diff | hist) . . (+551) . . 20.109(S21):Homework (→Due M1D4)
- 21:20, 22 January 2021 (diff | hist) . . (+548) . . 20.109(S21):Homework (→Due M1D3)
- 21:19, 22 January 2021 (diff | hist) . . (+703) . . 20.109(S21):Homework (→Due M1D3)
- 21:17, 22 January 2021 (diff | hist) . . (-1) . . 20.109(S21):Homework (→Due M1D2)
- 21:16, 22 January 2021 (diff | hist) . . (+201) . . 20.109(S21):Homework (→Due M1D2)
- 21:02, 22 January 2021 (diff | hist) . . (+8) . . 20.109(S21):Homework (→Due M1D2)
- 19:16, 22 January 2021 (diff | hist) . . (+2) . . 20.109(S21):M2D3 (→Part 4: Scan SMM slides)
- 19:15, 22 January 2021 (diff | hist) . . (+2) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 16:35, 22 January 2021 (diff | hist) . . (+20) . . 20.109(S21):M2D6 (→Navigation links)
- 16:35, 22 January 2021 (diff | hist) . . (+20) . . 20.109(S21):Module 2 (→Lab links: day by day)
- 16:34, 22 January 2021 (diff | hist) . . (+20) . . 20.109(S21):Spring 2021 schedule
- 16:30, 22 January 2021 (diff | hist) . . (+8,127) . . N 20.109(S21):M2D7 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== Traditionally, only one species of antibody could be used o...")
- 16:27, 22 January 2021 (diff | hist) . . (0) . . 20.109(S21):M2D6 (→Navigation links)
- 16:26, 22 January 2021 (diff | hist) . . (+11,192) . . N 20.109(S21):M2D6 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== At the start of the module, we discussed the DSF assay as it...")
- 16:04, 22 January 2021 (diff | hist) . . (-2) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 01:07, 22 January 2021 (diff | hist) . . (+33) . . 20.109(S21):M2D1 (→Navigation links)
- 01:05, 22 January 2021 (diff | hist) . . (+27) . . 20.109(S21):M1D7 (→Navigation links)
- 01:04, 22 January 2021 (diff | hist) . . (0) . . 20.109(S21):M1D7
- 01:04, 22 January 2021 (diff | hist) . . (0) . . 20.109(S21):M1D6
- 01:04, 22 January 2021 (diff | hist) . . (0) . . 20.109(S21):M1D4
- 01:03, 22 January 2021 (diff | hist) . . (-1) . . 20.109(S21):Module 1
- 00:59, 22 January 2021 (diff | hist) . . (+60) . . 20.109(S21):Module 1 (→Engineering Antibodies Using Yeast Display)
- 00:58, 22 January 2021 (diff | hist) . . (+20) . . 20.109(S21):Module 2 (→Overview)
- 00:57, 22 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2 overview schematic w labels.png (current)
- 00:55, 22 January 2021 (diff | hist) . . (+3,181) . . N 20.109(S21):Module 2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} =<center>Module 2</center>= '''Lecturer:''' [http://be.mit.edu/directory/ang...")
- 00:51, 22 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2 overview.pptx (current)
- 00:02, 22 January 2021 (diff | hist) . . (+1,226) . . 20.109(S21):Homework
- 23:55, 21 January 2021 (diff | hist) . . (-1) . . 20.109(S21):Homework (→Due M1D2)
- 23:54, 21 January 2021 (diff | hist) . . (+3,661) . . N 20.109(S21):Homework (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Module 1: Engineering antibodies== ===Due M1D1=== #Review the 20.109(S2...")
- 23:35, 21 January 2021 (diff | hist) . . (0) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 23:34, 21 January 2021 (diff | hist) . . (-34) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 23:32, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D2 SDSPAGE images.png (current)
- 23:00, 21 January 2021 (diff | hist) . . (+19) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 22:58, 21 January 2021 (diff | hist) . . (+12) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 22:42, 21 January 2021 (diff | hist) . . (-2) . . 20.109(S21):M2D5 (→Protocols)
- 22:41, 21 January 2021 (diff | hist) . . (+528) . . 20.109(S21):M2D5 (→Protocols)
- 22:33, 21 January 2021 (diff | hist) . . (-1) . . 20.109(S21):Guidelines for working in the tissue culture facility (current)
- 22:33, 21 January 2021 (diff | hist) . . (+7,247) . . N 20.109(S21):Guidelines for working in the tissue culture facility (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} Good cell culture technique will simultaneously protect you from anything dan...")
- 22:32, 21 January 2021 (diff | hist) . . (0) . . 20.109(S21):M2D5 (→Part 2: Learn cell culture best practices)
- 22:31, 21 January 2021 (diff | hist) . . (-12) . . 20.109(S21):M2D5 (→Introduction)
- 22:30, 21 January 2021 (diff | hist) . . (+27) . . 20.109(S21):M2D5 (→Introduction)
- 22:29, 21 January 2021 (diff | hist) . . (-42) . . 20.109(S21):M2D5 (→Introduction)
- 22:24, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D5 CAD cells.png (current)
- 22:06, 21 January 2021 (diff | hist) . . (-205) . . 20.109(S21):M2D4
- 22:06, 21 January 2021 (diff | hist) . . (0) . . 20.109(S21):M2D4 (→Part 3: Design experiments to test putative small molecule binders)
- 22:05, 21 January 2021 (diff | hist) . . (+6,789) . . 20.109(S21):M2D4 (→Protocols)
- 21:59, 21 January 2021 (diff | hist) . . (0) . . 20.109(S21):M2D4 (→Part 1: Identify hits from SMM results)
- 21:57, 21 January 2021 (diff | hist) . . (-2) . . 20.109(S21):M2D4 (→Part 1: Identify hits from SMM results)
- 21:56, 21 January 2021 (diff | hist) . . (-74) . . 20.109(S21):M2D4 (→Part 1: Identify hits from SMM results)
- 21:56, 21 January 2021 (diff | hist) . . (+188) . . 20.109(S21):M2D5 (→Protocols)
- 21:55, 21 January 2021 (diff | hist) . . (-190) . . 20.109(S21):M2D4 (→Protocols)
- 21:54, 21 January 2021 (diff | hist) . . (+6,089) . . N 20.109(S21):M2D4 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== Today is the culminating day of Module 2 and hopefully you ...")
- 21:48, 21 January 2021 (diff | hist) . . (+3) . . 20.109(S21):M2D3 (→Part 4: Scan SMM slides)
- 21:47, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D3 SMM complex, scan v2.png (current)
- 21:43, 21 January 2021 (diff | hist) . . (-32) . . 20.109(S21):M2D3 (→Protocols)
- 21:42, 21 January 2021 (diff | hist) . . (-49) . . 20.109(S21):M2D3 (→Reagents list)
- 21:42, 21 January 2021 (diff | hist) . . (-672) . . 20.109(S21):M2D3 (→Part 3: Perform SMM screen)
- 21:39, 21 January 2021 (diff | hist) . . (+105) . . 20.109(S21):M2D3 (→Reagents list)
- 21:37, 21 January 2021 (diff | hist) . . (+199) . . 20.109(S21):M2D3 (→Protocols)
- 21:37, 21 January 2021 (diff | hist) . . (+1,211) . . 20.109(S21):M2D3 (→Part 3: Scan SMM slides)
- 21:34, 21 January 2021 (diff | hist) . . (+13) . . 20.109(S21):M2D3 (→Part 3: Scan SMM slides)
- 21:31, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D3 SMM complex, scan.png (current)
- 21:13, 21 January 2021 (diff | hist) . . (-5) . . 20.109(S21):M2D3 (→Protocols)
- 20:53, 21 January 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D1
- 20:53, 21 January 2021 (diff | hist) . . (+318) . . 20.109(S21):M1D1 (→Protocols:)
- 20:52, 21 January 2021 (diff | hist) . . (-317) . . 20.109(S21):M2D5 (→Protocols)
- 20:52, 21 January 2021 (diff | hist) . . (+10,271) . . N 20.109(S21):M2D5 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== In the past century, we have learned a tremendous amount by ...")
- 20:40, 21 January 2021 (diff | hist) . . (+10,594) . . N 20.109(S21):M2D3 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== A small molecule microarray (SMM) requires the covalent atta...")
- 20:30, 21 January 2021 (diff | hist) . . (-137) . . 20.109(S21):M2D2 (→Part 3: Measure protein concentration)
- 20:27, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D2 BCA data.png
- 20:21, 21 January 2021 (diff | hist) . . (-13) . . 20.109(S21):M2D2 (→Navigation links)
- 20:18, 21 January 2021 (diff | hist) . . (+11) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:18, 21 January 2021 (diff | hist) . . (-11) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:15, 21 January 2021 (diff | hist) . . (+2) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:15, 21 January 2021 (diff | hist) . . (+103) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:12, 21 January 2021 (diff | hist) . . (+2) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:11, 21 January 2021 (diff | hist) . . (0) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:10, 21 January 2021 (diff | hist) . . (+233) . . 20.109(S21):M2D2 (→Part 2: Visualize TDP43-RRM12 protein using polyacrylamide gel electrophoresis (PAGE))
- 20:10, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D2 uninduced control SDSPAGE.png (current)
- 20:07, 21 January 2021 (diff | hist) . . (0) . . N File:Sp21 M2D2 protein purification SDSPAGE.png (current)
- 19:57, 21 January 2021 (diff | hist) . . (+15,777) . . N 20.109(S21):M2D2 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== Two metrics will be used to evaluate the success of the pro...")
- 19:46, 21 January 2021 (diff | hist) . . (+120) . . 20.109(S21):M2D1 (→Reagents list)
- 19:45, 21 January 2021 (diff | hist) . . (+22) . . 20.109(S21):M2D1 (→Purify TDP43-RRM12 from cell lysate)
- 19:44, 21 January 2021 (diff | hist) . . (-110) . . 20.109(S21):M2D1 (→Reagents list)
- 19:39, 21 January 2021 (diff | hist) . . (-2) . . 20.109(S21):M2D1 (→Part 2: Purify PF3D7_1351100 protein)
- 19:39, 21 January 2021 (diff | hist) . . (-2) . . 20.109(S21):M2D1 (→Introduction)
- 19:37, 21 January 2021 (diff | hist) . . (+1) . . 20.109(S21):M2D1 (→Purify TDP43-RRM12 from cell lysate)
- 19:36, 21 January 2021 (diff | hist) . . (-2) . . 20.109(S21):M2D1 (→Purify PF3D7_1351100 from cell lysate)
- 19:36, 21 January 2021 (diff | hist) . . (+12) . . 20.109(S21):M2D1 (→Purify PF3D7_1351100 from cell lysate)
- 19:34, 21 January 2021 (diff | hist) . . (+1) . . 20.109(S21):M2D1 (→Purify PF3D7_1351100 from cell lysate)
- 19:33, 21 January 2021 (diff | hist) . . (+809) . . 20.109(S21):M2D1 (→Part 2: Purify PF3D7_1351100 protein)
- 19:14, 21 January 2021 (diff | hist) . . (+180) . . 20.109(S21):M2D1 (→Part 1: Induce expression of TDP43-RRM12)
- 19:06, 21 January 2021 (diff | hist) . . (+601) . . 20.109(S21):M2D1 (→Introduction)
- 19:03, 21 January 2021 (diff | hist) . . (+10,773) . . N 20.109(S21):M2D1 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Introduction== To induce production of TDP43-RRM12 protein from the express...")
- 21:48, 19 January 2021 (diff | hist) . . (+38) . . 20.109(S21):Spring 2021 schedule
- 21:43, 19 January 2021 (diff | hist) . . (+135) . . 20.109(S21):Spring 2021 schedule
- 21:33, 19 January 2021 (diff | hist) . . (+17) . . 20.109(S21):Spring 2021 schedule
- 19:19, 19 January 2021 (diff | hist) . . (+1) . . 20.109(S21):Laboratory tour (→Laboratory Partner questionnaire)
- 19:18, 19 January 2021 (diff | hist) . . (0) . . 20.109(S21):Laboratory tour (→Prep notes)
- 19:18, 19 January 2021 (diff | hist) . . (-3) . . 20.109(S21):Laboratory tour (→Station 8: Introduction to course wiki)
- 19:17, 19 January 2021 (diff | hist) . . (0) . . N File:Sp21 toolbar image.png (current)
- 19:16, 19 January 2021 (diff | hist) . . (+13) . . 20.109(S21):Laboratory tour (→Lab Partner questionnaire)
- 19:15, 19 January 2021 (diff | hist) . . (-1) . . 20.109(S21):Laboratory tour (→Orientation questionnaire)
- 19:14, 19 January 2021 (diff | hist) . . (+27,923) . . N 20.109(S21):Laboratory tour (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} =Introduction= The main goals for today are to get to know one another and as...")
- 19:09, 19 January 2021 (diff | hist) . . (-4) . . 20.109(S21):Spring 2021 schedule
- 19:06, 19 January 2021 (diff | hist) . . (0) . . 20.109(S21):Announcements (→Office hours for Spring 2021)
- 19:02, 19 January 2021 (diff | hist) . . (+15) . . 20.109(S21):Communication (→Workshop Slides)
- 19:02, 19 January 2021 (diff | hist) . . (+13) . . 20.109(S21):Communication
- 18:58, 19 January 2021 (diff | hist) . . (+2,303) . . N 20.109(S21):Communication (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==BE Communication Lab information== Throughout the semester you will parti...")
- 18:52, 19 January 2021 (diff | hist) . . (-49) . . 20.109(S21):Assignments (→Overview)
- 18:51, 19 January 2021 (diff | hist) . . (-8) . . 20.109(S21):Assignments (→Participation and Blogging (5% of final grade))
- 18:50, 19 January 2021 (diff | hist) . . (-28) . . 20.109(S21):Assignments (→Participation and Blogging (5% of final grade))
- 18:49, 19 January 2021 (diff | hist) . . (+12,943) . . N 20.109(S21):Assignments (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Overview== You will complete three experimental modules over the course of...")
- 18:44, 19 January 2021 (diff | hist) . . (-26) . . 20.109(S21):Announcements (→Office hours for Spring 2021)
- 18:44, 19 January 2021 (diff | hist) . . (-1) . . 20.109(S21):Announcements (→Teaching team for Spring 2021)
- 18:43, 19 January 2021 (diff | hist) . . (+2,049) . . N 20.109(S21):Announcements (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #1e0c9f;"> {{Template:20.109(S21)}} ==Teaching team for Spring 2021== '''Teaching faculty:''' * [https://be.mit.e...")
- 17:05, 19 January 2021 (diff | hist) . . (+274) . . 20.109(S21):Spring 2021 schedule
- 17:13, 1 December 2020 (diff | hist) . . (+111) . . 20.109(F20): Fall 2020 schedule
- 20:19, 19 November 2020 (diff | hist) . . (+112) . . 20.109(F20): Fall 2020 schedule
- 20:58, 13 November 2020 (diff | hist) . . (+385) . . 20.109(S21):Spring 2021 schedule
- 20:56, 13 November 2020 (diff | hist) . . (-6,814) . . 20.109(S21):Spring 2021 schedule
- 00:05, 13 November 2020 (diff | hist) . . (-1,381) . . 20.109(S21):Spring 2021 schedule
- 23:55, 12 November 2020 (diff | hist) . . (-118) . . 20.109(S21):Spring 2021 schedule
- 23:55, 12 November 2020 (diff | hist) . . (+72) . . 20.109(S21):Spring 2021 schedule
- 23:52, 12 November 2020 (diff | hist) . . (-6,454) . . 20.109(S21):Spring 2021 schedule
- 23:32, 12 November 2020 (diff | hist) . . (-157) . . 20.109(S21):Spring 2021 schedule
- 23:32, 12 November 2020 (diff | hist) . . (+7) . . 20.109(S21):Spring 2021 schedule
- 23:31, 12 November 2020 (diff | hist) . . (+123) . . 20.109(S21):Spring 2021 schedule
- 23:21, 12 November 2020 (diff | hist) . . (+3) . . 20.109(S21):Spring 2021 schedule
- 23:19, 12 November 2020 (diff | hist) . . (0) . . 20.109(S21):Spring 2021 schedule
- 23:18, 12 November 2020 (diff | hist) . . (+1) . . Template:20.109(S21)
- 23:17, 12 November 2020 (diff | hist) . . (+1) . . Template:20.109(S21)
- 23:17, 12 November 2020 (diff | hist) . . (+1) . . Template:20.109(S21)
- 23:15, 12 November 2020 (diff | hist) . . (-1) . . Template:20.109(S21)
- 23:14, 12 November 2020 (diff | hist) . . (+2) . . Template:20.109(S21)
- 23:13, 12 November 2020 (diff | hist) . . (0) . . Template:20.109(S21)
- 23:01, 12 November 2020 (diff | hist) . . (+3) . . Template:20.109(S21)
- 23:01, 12 November 2020 (diff | hist) . . (0) . . N File:Sp21 banner image v2.png (current)
- 22:59, 12 November 2020 (diff | hist) . . (-3) . . Template:20.109(S21)
- 22:58, 12 November 2020 (diff | hist) . . (0) . . N File:Sp21 banner image.png (current)
- 19:14, 12 November 2020 (diff | hist) . . (+21,373) . . N 20.109(S21):Spring 2021 schedule (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #0489B1;"> {{Template:20.109(S21)}} Welcome to 20.109! It is our goal to make this class a useful and fun introdu...")
- 19:06, 12 November 2020 (diff | hist) . . (0) . . Template:20.109(S21)
- 19:05, 12 November 2020 (diff | hist) . . (+2,060) . . N Template:20.109(S21) (Created page with "Category: 20.109ExportF20 <div class=noprint> <!-- This div tag ensures that the header won't be printed when students go to print course materials from the site. --> <d...")
- 17:10, 12 November 2020 (diff | hist) . . (+111) . . 20.109(F20): Fall 2020 schedule
- 20:38, 5 November 2020 (diff | hist) . . (+82) . . 20.109(F20): Fall 2020 schedule
- 20:37, 5 November 2020 (diff | hist) . . (+208) . . 20.109(F20): Fall 2020 schedule
- 20:36, 5 November 2020 (diff | hist) . . (+211) . . 20.109(F20): Fall 2020 schedule
- 20:41, 3 November 2020 (diff | hist) . . (-5) . . 20.109(F20): Fall 2020 schedule
- 20:40, 3 November 2020 (diff | hist) . . (+167) . . 20.109(F20): Fall 2020 schedule
- 20:02, 3 November 2020 (diff | hist) . . (+112) . . 20.109(F20): Fall 2020 schedule
- 14:20, 3 November 2020 (diff | hist) . . (+97) . . 20.109(F20): Fall 2020 schedule
- 17:10, 29 October 2020 (diff | hist) . . (+418) . . 20.109(F20): Fall 2020 schedule
- 21:24, 26 October 2020 (diff | hist) . . (+201) . . 20.109(F20):M2D7 (→Part 2: Design experiments to test putative small molecule binders)
- 21:21, 26 October 2020 (diff | hist) . . (+31) . . 20.109(F20):M2D7 (→Part 1: Visually evaluate chemical structures of positive hits)
- 21:20, 26 October 2020 (diff | hist) . . (+1,943) . . 20.109(F20):M2D7 (→Protocols)
- 21:08, 26 October 2020 (diff | hist) . . (+2,634) . . 20.109(F20):M2D7 (→Introduction)
- 21:04, 26 October 2020 (diff | hist) . . (-2) . . 20.109(F20): Fall 2020 schedule
- 20:13, 23 October 2020 (diff | hist) . . (0) . . 20.109(F20): Fall 2020 schedule
- 15:30, 16 October 2020 (diff | hist) . . (+71) . . 20.109(F20): Fall 2020 schedule
- 19:23, 15 October 2020 (diff | hist) . . (+208) . . 20.109(F20): Fall 2020 schedule
- 18:23, 9 October 2020 (diff | hist) . . (+71) . . 20.109(F20): Fall 2020 schedule
- 18:21, 9 October 2020 (diff | hist) . . (+209) . . 20.109(F20): Fall 2020 schedule
- 16:02, 7 October 2020 (diff | hist) . . (+36) . . Template:20.109(F20) (current)
- 16:01, 7 October 2020 (diff | hist) . . (+2) . . Template:20.109(F20)
- 15:59, 7 October 2020 (diff | hist) . . (0) . . Template:20.109(F20)
- 15:58, 7 October 2020 (diff | hist) . . (+112) . . Template:20.109(F20)
- 13:04, 7 October 2020 (diff | hist) . . (+1) . . 20.109(F20): Fall 2020 schedule
- 13:03, 7 October 2020 (diff | hist) . . (+23) . . 20.109(F20): Fall 2020 schedule
- 13:02, 7 October 2020 (diff | hist) . . (0) . . N File:Fa20 M2D3 nll.pdf (current)
- 02:12, 7 October 2020 (diff | hist) . . (+208) . . 20.109(F20): Fall 2020 schedule
- 16:17, 2 October 2020 (diff | hist) . . (+71) . . 20.109(F20): Fall 2020 schedule
- 15:48, 2 October 2020 (diff | hist) . . (+36) . . 20.109(F20): Assignments (→Participation and Blogging (5% of final grade)) (current)
- 15:48, 2 October 2020 (diff | hist) . . (+144) . . 20.109(F20): Fall 2020 schedule
- 23:00, 1 October 2020 (diff | hist) . . (+1) . . 20.109(F20): Fall 2020 schedule
- 23:00, 1 October 2020 (diff | hist) . . (+207) . . 20.109(F20): Fall 2020 schedule
- 15:27, 1 October 2020 (diff | hist) . . (-2) . . 20.109(F20):Module 2 (→Overview)
- 15:17, 30 September 2020 (diff | hist) . . (+232) . . 20.109(F20): Fall 2020 schedule
- 14:03, 28 September 2020 (diff | hist) . . (-22) . . 20.109(F20):Module 2 (→Lab links: day by day)
- 14:00, 28 September 2020 (diff | hist) . . (+1) . . 20.109(F20):Module 2 (→Overview)
- 14:00, 28 September 2020 (diff | hist) . . (-202) . . 20.109(F20):Module 2 (→Overview)
- 13:59, 28 September 2020 (diff | hist) . . (+129) . . 20.109(F20):Module 2 (→Overview)
- 13:58, 28 September 2020 (diff | hist) . . (+1,045) . . 20.109(F20):Module 2 (→Overview)
- 18:52, 25 September 2020 (diff | hist) . . (+10) . . 20.109(F20): Fall 2020 schedule
- 16:03, 25 September 2020 (diff | hist) . . (+24) . . 20.109(F20): Fall 2020 schedule
- 16:02, 25 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D7 nll.pdf (current)
- 15:55, 24 September 2020 (diff | hist) . . (+344) . . 20.109(F20):M1D7 (→Part 2: Complete data analysis) (current)
- 15:36, 23 September 2020 (diff | hist) . . (+24) . . 20.109(F20): Fall 2020 schedule
- 15:36, 23 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D6 nll.pdf (current)
- 12:52, 16 September 2020 (diff | hist) . . (+24) . . 20.109(F20): Fall 2020 schedule
- 12:51, 16 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D4 nll.pdf (current)
- 16:45, 14 September 2020 (diff | hist) . . (0) . . 20.109(F20):M1D4 (→Part 4: Analyze γH2AX images by counting foci)
- 16:44, 14 September 2020 (diff | hist) . . (+273) . . 20.109(F20):M1D4 (→Protocols)
- 16:43, 14 September 2020 (diff | hist) . . (+334) . . 20.109(F20):M1D4 (→Part 4: Analyze γH2AX images by counting foci)
- 16:38, 14 September 2020 (diff | hist) . . (+76) . . 20.109(F20):M1D4 (→Part 4: Analyze γH2AX images by measuring fluorescence intensity)
- 16:37, 14 September 2020 (diff | hist) . . (+95) . . 20.109(F20):M1D4 (→Protocols)
- 14:27, 11 September 2020 (diff | hist) . . (+24) . . 20.109(F20): Fall 2020 schedule
- 14:27, 11 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D3 nll.pdf (current)
- 23:14, 10 September 2020 (diff | hist) . . (-110) . . 20.109(F20):M1D3 (→Part 1: Begin antibody staining for γH2AX assay) (current)
- 18:28, 9 September 2020 (diff | hist) . . (0) . . File:Fa20 M1D2 nll.pdf (Noreen Lyell uploaded a new version of "File:Fa20 M1D2 nll.pdf") (current)
- 13:08, 9 September 2020 (diff | hist) . . (+24) . . 20.109(F20): Fall 2020 schedule
- 13:07, 9 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D2 nll.pdf
- 23:47, 8 September 2020 (diff | hist) . . (+23) . . 20.109(F20):M1D2 (→Introduction)
- 21:08, 4 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D1 nll marked.pdf (current)
- 15:34, 4 September 2020 (diff | hist) . . (+1) . . 20.109(F20): Fall 2020 schedule
- 15:34, 4 September 2020 (diff | hist) . . (+23) . . 20.109(F20): Fall 2020 schedule
- 15:33, 4 September 2020 (diff | hist) . . (0) . . N File:Fa20 M1D1 nll.pdf (current)
- 17:47, 2 September 2020 (diff | hist) . . (-29) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 17:46, 2 September 2020 (diff | hist) . . (-26) . . 20.109(F20):M2D1 (→Part 3: Ligation of PF3D7_1351100 insert and pET-28b(+) expression vector)
- 17:45, 2 September 2020 (diff | hist) . . (-3) . . 20.109(F20):M2D1 (→Introduction)
- 17:45, 2 September 2020 (diff | hist) . . (-4) . . 20.109(F20):M2D1 (→Introduction)
- 17:43, 2 September 2020 (diff | hist) . . (+5) . . 20.109(F20):M1D6 (→Introduction)
- 13:00, 2 September 2020 (diff | hist) . . (+129) . . 20.109(F20): Fall 2020 schedule
- 18:02, 1 September 2020 (diff | hist) . . (+44) . . 20.109(F20): Fall 2020 schedule
- 18:01, 1 September 2020 (diff | hist) . . (0) . . N File:Fa20 Orientation lecture.pdf (current)
- 17:48, 1 September 2020 (diff | hist) . . (+134) . . 20.109(F20): Fall 2020 schedule
- 16:04, 1 September 2020 (diff | hist) . . (0) . . 20.109(F20): Fall 2020 schedule
- 23:45, 31 August 2020 (diff | hist) . . (0) . . 20.109(F20): Fall 2020 schedule
- 23:41, 31 August 2020 (diff | hist) . . (+42) . . 20.109(F20): Announcements (→Office hours for Fall 2020)
- 23:40, 31 August 2020 (diff | hist) . . (+19) . . 20.109(F20): Announcements (→Teaching team for Fall 2020)
- 23:40, 31 August 2020 (diff | hist) . . (+57) . . 20.109(F20): Fall 2020 schedule
- 23:39, 31 August 2020 (diff | hist) . . (+1,124) . . 20.109(F20): Fall 2020 schedule
- 23:36, 31 August 2020 (diff | hist) . . (+47) . . 20.109(F20): Fall 2020 schedule
- 20:40, 3 August 2020 (diff | hist) . . (+5) . . In silico cloning of pdCas9 construct (→Step 1: Amplification) (current)
- 18:28, 3 August 2020 (diff | hist) . . (+328) . . In silico cloning of pdCas9 construct (→Related module)
- 18:00, 3 August 2020 (diff | hist) . . (-1) . . In silico cloning of pdCas9 construct (→References)
- 17:59, 3 August 2020 (diff | hist) . . (0) . . In silico cloning of pdCas9 construct (→References)
- 17:57, 3 August 2020 (diff | hist) . . (-530) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 17:53, 3 August 2020 (diff | hist) . . (+223) . . In silico cloning of pdCas9 construct (→Step 2: Digestion)
- 17:47, 3 August 2020 (diff | hist) . . (+8) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 17:45, 3 August 2020 (diff | hist) . . (-29) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 17:36, 3 August 2020 (diff | hist) . . (+4) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 17:35, 3 August 2020 (diff | hist) . . (+193) . . In silico cloning of pdCas9 construct (→References)
- 17:31, 3 August 2020 (diff | hist) . . (+13) . . In silico cloning of pdCas9 construct (→Overview)
- 17:29, 3 August 2020 (diff | hist) . . (+80) . . In silico cloning of pdCas9 construct (→Overview)
- 17:25, 3 August 2020 (diff | hist) . . (+582) . . In silico cloning of pdCas9 construct (→Overview)
- 16:54, 3 August 2020 (diff | hist) . . (+4) . . In silico cloning of pdCas9 construct (→Overview)
- 16:53, 3 August 2020 (diff | hist) . . (+573) . . In silico cloning of pdCas9 construct (→Overview)
- 16:44, 3 August 2020 (diff | hist) . . (-1) . . In silico cloning of pdCas9 construct (→Overview)
- 16:42, 3 August 2020 (diff | hist) . . (-3) . . In silico cloning of pdCas9 construct (→References)
- 16:41, 3 August 2020 (diff | hist) . . (-4) . . In silico cloning of pdCas9 construct (→Overview)
- 16:41, 3 August 2020 (diff | hist) . . (+215) . . In silico cloning of pdCas9 construct (→References)
- 15:58, 3 August 2020 (diff | hist) . . (-2) . . In silico cloning of pdCas9 construct (→Overview)
- 15:57, 3 August 2020 (diff | hist) . . (+6) . . In silico cloning of pdCas9 construct (→References)
- 15:56, 3 August 2020 (diff | hist) . . (-1) . . In silico cloning of pdCas9 construct (→References)
- 15:56, 3 August 2020 (diff | hist) . . (+164) . . In silico cloning of pdCas9 construct (→References)
- 15:52, 3 August 2020 (diff | hist) . . (+6) . . In silico cloning of pdCas9 construct (→Overview)
- 15:51, 3 August 2020 (diff | hist) . . (+4) . . In silico cloning of pdCas9 construct (→References)
- 15:50, 3 August 2020 (diff | hist) . . (-2) . . In silico cloning of pdCas9 construct (→Overview)
- 15:48, 3 August 2020 (diff | hist) . . (+148) . . In silico cloning of pdCas9 construct (→References)
- 15:42, 3 August 2020 (diff | hist) . . (+178) . . In silico cloning of pdCas9 construct (→References)
- 15:37, 3 August 2020 (diff | hist) . . (+161) . . In silico cloning of pdCas9 construct (→References)
- 15:31, 3 August 2020 (diff | hist) . . (+1) . . In silico cloning of pdCas9 construct (→References)
- 15:30, 3 August 2020 (diff | hist) . . (-3) . . In silico cloning of pdCas9 construct (→References)
- 15:30, 3 August 2020 (diff | hist) . . (+566) . . In silico cloning of pdCas9 construct (→References)
- 15:19, 3 August 2020 (diff | hist) . . (+69) . . In silico cloning of pdCas9 construct (→Overview)
- 14:54, 3 August 2020 (diff | hist) . . (+181) . . In silico cloning of pdCas9 construct
- 14:05, 3 August 2020 (diff | hist) . . (+22) . . 20.109(F20):Module 2 (→Lab links: day by day)
- 23:58, 2 August 2020 (diff | hist) . . (-3) . . In silico cloning of pdCas9 construct (→Related modules)
- 23:58, 2 August 2020 (diff | hist) . . (+4) . . In silico cloning of pdCas9 construct (→Related modules)
- 23:57, 2 August 2020 (diff | hist) . . (+578) . . In silico cloning of pdCas9 construct (→Overview)
- 23:54, 2 August 2020 (diff | hist) . . (-294) . . In silico cloning of pdCas9 construct (→Related modules)
- 23:53, 2 August 2020 (diff | hist) . . (-1,990) . . In silico cloning of pdCas9 construct (→Step 4: Confirmation)
- 22:02, 2 August 2020 (diff | hist) . . (-26) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 22:02, 2 August 2020 (diff | hist) . . (-96) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 21:03, 2 August 2020 (diff | hist) . . (-209) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 21:01, 2 August 2020 (diff | hist) . . (-309) . . In silico cloning of pdCas9 construct (→Reagents)
- 20:57, 2 August 2020 (diff | hist) . . (-1) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 20:56, 2 August 2020 (diff | hist) . . (-1) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 20:56, 2 August 2020 (diff | hist) . . (-574) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 20:52, 2 August 2020 (diff | hist) . . (+5) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 20:51, 2 August 2020 (diff | hist) . . (+17) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 20:46, 2 August 2020 (diff | hist) . . (-95) . . In silico cloning of pdCas9 construct (→Step 3: Ligation)
- 20:42, 2 August 2020 (diff | hist) . . (-308) . . In silico cloning of pdCas9 construct (→Step 2: Digestion)
- 20:27, 2 August 2020 (diff | hist) . . (+128) . . In silico cloning of pdCas9 construct (→Step 2: Digestion)
- 20:23, 2 August 2020 (diff | hist) . . (0) . . In silico cloning of pdCas9 construct (→Step 2: Digestion)
- 20:22, 2 August 2020 (diff | hist) . . (-845) . . In silico cloning of pdCas9 construct (→Step 2: Digestion)
- 20:22, 2 August 2020 (diff | hist) . . (0) . . N File:NLL expression vector sequence.docx (current)
- 20:15, 2 August 2020 (diff | hist) . . (+4) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 20:13, 2 August 2020 (diff | hist) . . (+124) . . In silico cloning of pdCas9 construct (→Step 2: Digestion)
- 20:13, 2 August 2020 (diff | hist) . . (-223) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 20:12, 2 August 2020 (diff | hist) . . (-135) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 20:04, 2 August 2020 (diff | hist) . . (0) . . N File:NLL dCas9 sequence.docx (current)
- 19:59, 2 August 2020 (diff | hist) . . (-1) . . In silico cloning of pdCas9 construct (→Laboratory exercises)
- 19:58, 2 August 2020 (diff | hist) . . (+258) . . In silico cloning of pdCas9 construct (→Laboratory exercises)
- 19:57, 2 August 2020 (diff | hist) . . (-190) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 19:57, 2 August 2020 (diff | hist) . . (-232) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 19:45, 2 August 2020 (diff | hist) . . (-65) . . In silico cloning of pdCas9 construct (→Step 1: Amplification)
- 19:34, 2 August 2020 (diff | hist) . . (-27) . . In silico cloning of pdCas9 construct
- 19:32, 2 August 2020 (diff | hist) . . (+98) . . In silico cloning of pdCas9 construct (→Overview)
- 19:28, 2 August 2020 (diff | hist) . . (+1) . . In silico cloning of pdCas9 construct
- 19:28, 2 August 2020 (diff | hist) . . (0) . . N File:NLL pdCas9 cloning schematic.png (current)
- 19:14, 2 August 2020 (diff | hist) . . (0) . . In silico cloning of pdCas9 construct
- 19:13, 2 August 2020 (diff | hist) . . (+42) . . In silico cloning of pdCas9 construct (→Overview)
- 18:16, 2 August 2020 (diff | hist) . . (-2,041) . . In silico cloning of pdCas9 construct (→Step 4: Confirmation digest)
- 18:11, 2 August 2020 (diff | hist) . . (0) . . In silico cloning of pdCas9 construct (→Overview)
- 18:09, 2 August 2020 (diff | hist) . . (-70) . . In silico cloning of pdCas9 construct (→Overview)
- 17:57, 2 August 2020 (diff | hist) . . (+49) . . In silico cloning of pdCas9 construct (→Step 4: Confirmation digest)
- 17:55, 2 August 2020 (diff | hist) . . (+21,969) . . In silico cloning of pdCas9 construct (→Overview)
- 14:47, 2 August 2020 (diff | hist) . . (+345) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Overview) (current)
- 14:44, 2 August 2020 (diff | hist) . . (+9) . . Template:LaboratoryExercisesbyNLL (current)
- 14:44, 2 August 2020 (diff | hist) . . (0) . . Template:LaboratoryExercisesbyNLL
- 14:43, 2 August 2020 (diff | hist) . . (0) . . Template:LaboratoryExercisesbyNLL
- 14:43, 2 August 2020 (diff | hist) . . (0) . . Template:LaboratoryExercisesbyNLL
- 14:40, 2 August 2020 (diff | hist) . . (-17) . . Template:LaboratoryExercisesbyNLL
- 14:39, 2 August 2020 (diff | hist) . . (+15) . . Template:LaboratoryExercisesbyNLL
- 14:35, 2 August 2020 (diff | hist) . . (-1) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Related modules)
- 14:35, 2 August 2020 (diff | hist) . . (+542) . . N In silico cloning of pdCas9 construct (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #0404B4;"> {{Template:LaboratoryExercisesbyNLL}} ==Overview== ==Laboratory exercises== ==Related modules== #...")
- 14:32, 2 August 2020 (diff | hist) . . (+37) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Related modules)
- 14:30, 2 August 2020 (diff | hist) . . (+20) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Related modules)
- 14:28, 2 August 2020 (diff | hist) . . (+5) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Related modules)
- 14:28, 2 August 2020 (diff | hist) . . (+2) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Related modules)
- 14:27, 2 August 2020 (diff | hist) . . (+637) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Related modules)
- 14:12, 2 August 2020 (diff | hist) . . (+25) . . Using CRISPRi to increase ethanol yield in E. coli MG1655 (→Laboratory exercises)
- 14:11, 2 August 2020 (diff | hist) . . (+13) . . Using CRISPRi to increase ethanol yield in E. coli MG1655
- 14:10, 2 August 2020 (diff | hist) . . (+46) . . Template:LaboratoryExercisesbyNLL
- 14:09, 2 August 2020 (diff | hist) . . (0) . . Template:LaboratoryExercisesbyNLL
- 14:09, 2 August 2020 (diff | hist) . . (-1,254) . . Template:LaboratoryExercisesbyNLL
- 14:06, 2 August 2020 (diff | hist) . . (0) . . N File:Banner LaboratoryExcercisesbyNLL.png (current)
- 13:59, 2 August 2020 (diff | hist) . . (+2,081) . . N Template:LaboratoryExercisesbyNLL (Created page with "Category: 20.109ExportS19 <div class=noprint> <!-- This div tag ensures that the header won't be printed when students go to print course materials from the site. --> <d...")
- 13:52, 2 August 2020 (diff | hist) . . (+304) . . N Using CRISPRi to increase ethanol yield in E. coli MG1655 (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #0404B4;"> {{Template:20.109(S19)}} ==Overview== ==Laboratory exercises== Day 1: Day 2: Day 3: Day 4: Day 5: ...")
- 13:49, 2 August 2020 (diff | hist) . . (-201) . . CRISPRi module, Part II: Increasing ethanol yield in E. coli MG1655 (Blanked the page) (current)
- 13:26, 2 August 2020 (diff | hist) . . (+201) . . N CRISPRi module, Part II: Increasing ethanol yield in E. coli MG1655 (Created page with " ==Introduction== ==Protocols== ==Related modules== CRISPRi module, Part I: In silico cloning of pdCas9 construct CRISPRi module, Part III: Optimizing sgRNA design to furt...")
- 11:35, 31 July 2020 (diff | hist) . . (-78) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 11:31, 31 July 2020 (diff | hist) . . (+26) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 00:15, 31 July 2020 (diff | hist) . . (0) . . 20.109(F20):M2D3 (→Part 3: Measure protein concentration)
- 00:08, 31 July 2020 (diff | hist) . . (+395) . . 20.109(F20):M3D5 (→Part 2: Design optimized sgRNA_target for improved ethanol yield) (current)
- 00:03, 31 July 2020 (diff | hist) . . (+123) . . 20.109(F20):M3D5 (→Part 2: Design optimized sgRNA_target for improved ethanol yield)
- 23:58, 30 July 2020 (diff | hist) . . (+19) . . 20.109(F20):M3D2 (→Part 4: Align sgRNA_target sequences to host genome) (current)
- 21:05, 30 July 2020 (diff | hist) . . (+29) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 21:00, 30 July 2020 (diff | hist) . . (+603) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:51, 30 July 2020 (diff | hist) . . (+35) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:49, 30 July 2020 (diff | hist) . . (+71) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:48, 30 July 2020 (diff | hist) . . (+45) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:45, 30 July 2020 (diff | hist) . . (+493) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:40, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:39, 30 July 2020 (diff | hist) . . (+533) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:38, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 PWM table example.png (current)
- 20:29, 30 July 2020 (diff | hist) . . (+972) . . 20.109(F20):M3D4 (→Protocols)
- 20:28, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 M3D4 PWM example.png (current)
- 20:10, 30 July 2020 (diff | hist) . . (+96) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:09, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:09, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Protocols)
- 20:08, 30 July 2020 (diff | hist) . . (+80) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 20:08, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 M3D4 virtual footprint example.png (current)
- 19:56, 30 July 2020 (diff | hist) . . (+68) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 19:45, 30 July 2020 (diff | hist) . . (+483) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 19:33, 30 July 2020 (diff | hist) . . (+844) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 19:12, 30 July 2020 (diff | hist) . . (-92) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 19:11, 30 July 2020 (diff | hist) . . (+92) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 19:09, 30 July 2020 (diff | hist) . . (-4) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 19:07, 30 July 2020 (diff | hist) . . (+435) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 18:57, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 18:57, 30 July 2020 (diff | hist) . . (+56) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 18:56, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 M3D4 E. coli promoter.png (current)
- 18:51, 30 July 2020 (diff | hist) . . (+325) . . 20.109(F20):M3D4 (→Part 1: Examine targeted gene sequences for regulatory binding sites)
- 18:35, 30 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M3D4 (→Protocols)
- 18:34, 30 July 2020 (diff | hist) . . (+20) . . 20.109(F20):M3D4 (→Introduction)
- 18:30, 30 July 2020 (diff | hist) . . (-28) . . 20.109(F20):M3D4 (→Introduction)
- 18:27, 30 July 2020 (diff | hist) . . (+14) . . 20.109(F20):M3D4 (→Introduction)
- 18:26, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Introduction)
- 18:26, 30 July 2020 (diff | hist) . . (+686) . . 20.109(F20):M3D4 (→Introduction)
- 18:13, 30 July 2020 (diff | hist) . . (+6) . . 20.109(F20):M3D4 (→Part 3: Consider societal implications of your Research proposal)
- 17:54, 30 July 2020 (diff | hist) . . (+81) . . 20.109(F20):M3D4 (→Protocols)
- 17:46, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Introduction)
- 17:45, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Introduction)
- 17:44, 30 July 2020 (diff | hist) . . (+122) . . 20.109(F20):M3D4 (→Introduction)
- 17:43, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 M3D4 transcription factor binding.png (current)
- 17:28, 30 July 2020 (diff | hist) . . (+237) . . 20.109(F20):M3D4 (→Introduction)
- 17:20, 30 July 2020 (diff | hist) . . (+15) . . 20.109(F20):M3D4 (→Introduction)
- 17:18, 30 July 2020 (diff | hist) . . (+432) . . 20.109(F20):M3D4 (→Introduction)
- 17:00, 30 July 2020 (diff | hist) . . (+1,002) . . 20.109(F20):M3D4 (→Introduction)
- 16:42, 30 July 2020 (diff | hist) . . (+109) . . 20.109(F20):M3D4 (→Introduction)
- 16:39, 30 July 2020 (diff | hist) . . (+3) . . 20.109(F20):M3D4 (→Introduction)
- 16:39, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 M3D4 RNAP and sigma factor v2.png (current)
- 15:51, 30 July 2020 (diff | hist) . . (+12) . . 20.109(F20):M3D4 (→Introduction)
- 15:50, 30 July 2020 (diff | hist) . . (+566) . . 20.109(F20):M3D4 (→Introduction)
- 15:41, 30 July 2020 (diff | hist) . . (+27) . . 20.109(F20):M3D4 (→Introduction)
- 15:39, 30 July 2020 (diff | hist) . . (+14) . . 20.109(F20):M3D4 (→Introduction)
- 15:37, 30 July 2020 (diff | hist) . . (+408) . . 20.109(F20):M3D4 (→Introduction)
- 15:31, 30 July 2020 (diff | hist) . . (0) . . N File:Fa20 M3D4 RNAP and sigma factor.png (current)
- 14:16, 30 July 2020 (diff | hist) . . (+1) . . 20.109(F20):M3D4 (→Protocols)
- 14:12, 30 July 2020 (diff | hist) . . (-110) . . 20.109(F20):M3D4 (→Part 1: Examine DNA features in targeted gene sequences)
- 14:10, 30 July 2020 (diff | hist) . . (+143) . . 20.109(F20):M3D3 (→Part 2: Analyze ethanol yield data)
- 14:06, 30 July 2020 (diff | hist) . . (+162) . . 20.109(F20):M3D2 (→Part 4: Align sgRNA_target sequences to host genome)
- 14:03, 30 July 2020 (diff | hist) . . (+76) . . 20.109(F20):M3D2 (→Part 4: Align sgRNA_target sequences to host genome)
- 12:55, 30 July 2020 (diff | hist) . . (+8) . . 20.109(F20):M3D4 (→Part 1: Examine DNA features in genomic targets)
- 12:54, 30 July 2020 (diff | hist) . . (-119) . . 20.109(F20):M3D4 (→Part 1: Examine DNA features in genomic targets)
- 12:36, 30 July 2020 (diff | hist) . . (+705) . . 20.109(F20):M3D4 (→Part 1: Examine DNA features in genomic targets)
- 12:32, 30 July 2020 (diff | hist) . . (0) . . 20.109(F20):M3D4 (→Part 1: Examine DNA features in genomic targets)
- 11:50, 30 July 2020 (diff | hist) . . (+875) . . 20.109(F20):M3D4 (→Protocols)
- 20:37, 29 July 2020 (diff | hist) . . (-40) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 20:36, 29 July 2020 (diff | hist) . . (-45) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 20:23, 29 July 2020 (diff | hist) . . (+1) . . 20.109(F20):M1D7 (→Part 2: Complete data analysis)
- 20:21, 29 July 2020 (diff | hist) . . (+330) . . 20.109(F20):M1D7 (→Part 2: Complete data analysis)
- 20:18, 29 July 2020 (diff | hist) . . (+785) . . 20.109(F20):M1D7 (→Part 2: Complete data analysis)
- 20:14, 29 July 2020 (diff | hist) . . (0) . . N File:Fa20 M1D7 CometChip data dataset2.xlsx (current)
- 19:58, 29 July 2020 (diff | hist) . . (+119) . . 20.109(F20):M1D7 (→Introduction)
- 19:41, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M2D6 (→Introduction)
- 19:41, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M2D6 (→Introduction)
- 19:40, 29 July 2020 (diff | hist) . . (+315) . . 20.109(F20):M2D6 (→Part 2: Identify hits from SMM results)
- 19:36, 29 July 2020 (diff | hist) . . (-19) . . 20.109(F20):M2D6 (→Reagents list)
- 19:36, 29 July 2020 (diff | hist) . . (+1,327) . . 20.109(F20):M2D6 (→Protocols)
- 19:31, 29 July 2020 (diff | hist) . . (+26) . . 20.109(F20):M2D6 (→Introduction)
- 19:31, 29 July 2020 (diff | hist) . . (-26) . . 20.109(F20):M2D6 (→Introduction)
- 19:31, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M2D6 (→Introduction)
- 19:30, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M2D6 (→Introduction)
- 19:30, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M2D6 (→Introduction)
- 19:30, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M2D6 (→Introduction)
- 19:30, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M2D6 (→Introduction)
- 19:29, 29 July 2020 (diff | hist) . . (+27) . . 20.109(F20):M2D6 (→Introduction)
- 19:29, 29 July 2020 (diff | hist) . . (+755) . . 20.109(F20):M2D6 (→Introduction)
- 18:41, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 18:02, 29 July 2020 (diff | hist) . . (+1) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 17:57, 29 July 2020 (diff | hist) . . (+82) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 17:56, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 17:55, 29 July 2020 (diff | hist) . . (+1,083) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 17:41, 29 July 2020 (diff | hist) . . (0) . . N File:Fa20 M1D6 CometChip data.xlsx (current)
- 17:07, 29 July 2020 (diff | hist) . . (+35) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 17:05, 29 July 2020 (diff | hist) . . (+1,396) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 16:29, 29 July 2020 (diff | hist) . . (-360) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 16:25, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 16:24, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 16:24, 29 July 2020 (diff | hist) . . (-119) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 16:19, 29 July 2020 (diff | hist) . . (+18) . . 20.109(F20):M1D7 (→Introduction)
- 16:16, 29 July 2020 (diff | hist) . . (+66) . . 20.109(F20):M1D7 (→Part 2: Complete data analysis)
- 16:07, 29 July 2020 (diff | hist) . . (+831) . . 20.109(F20):M1D7 (→Introduction)
- 15:53, 29 July 2020 (diff | hist) . . (+9) . . 20.109(F20):M1D7 (→Introduction)
- 15:49, 29 July 2020 (diff | hist) . . (+10) . . 20.109(F20):M1D7 (→Introduction)
- 15:44, 29 July 2020 (diff | hist) . . (-88) . . 20.109(F20):M1D6 (→Part 2: Analyze CometChip images)
- 15:26, 29 July 2020 (diff | hist) . . (+463) . . 20.109(F20):M1D2 (→Part 2: Prepare coverslips for γH2AX assay)
- 15:24, 29 July 2020 (diff | hist) . . (+60) . . 20.109(F20):M1D2 (→Protocols)
- 02:09, 29 July 2020 (diff | hist) . . (+1) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 02:07, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M1D2 (→Part 2: Define treatment conditions that will be used for γH2AX experiment)
- 02:07, 29 July 2020 (diff | hist) . . (-726) . . 20.109(F20):M1D2 (→Part 1: Seed cells for γH2AX assay)
- 02:04, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M1D2 (→Reagents list)
- 02:03, 29 July 2020 (diff | hist) . . (-76) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 02:01, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 02:01, 29 July 2020 (diff | hist) . . (+3) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 02:01, 29 July 2020 (diff | hist) . . (+703) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 01:51, 29 July 2020 (diff | hist) . . (+244) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 01:41, 29 July 2020 (diff | hist) . . (-239) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 01:41, 29 July 2020 (diff | hist) . . (+338) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 01:36, 29 July 2020 (diff | hist) . . (-11) . . 20.109(F20):M1D5 (→Reagents list)
- 01:35, 29 July 2020 (diff | hist) . . (+47) . . 20.109(F20):M1D5 (→Reagents list)
- 01:33, 29 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 01:31, 29 July 2020 (diff | hist) . . (-32) . . 20.109(F20):M1D5 (→Reagents list)
- 01:28, 29 July 2020 (diff | hist) . . (-7) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 01:27, 29 July 2020 (diff | hist) . . (+3) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 01:26, 29 July 2020 (diff | hist) . . (+278) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 01:10, 29 July 2020 (diff | hist) . . (-1,636) . . 20.109(F20):M1D5 (→Treat MCL-5 cells with H2O2)
- 01:02, 29 July 2020 (diff | hist) . . (-3) . . 20.109(F20):M1D5 (→Expose MCL-5 cells to As)
- 01:00, 29 July 2020 (diff | hist) . . (+33) . . 20.109(F20):M1D5 (→Expose MCL-5 cells to As)
- 01:00, 29 July 2020 (diff | hist) . . (+604) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 00:43, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 00:42, 29 July 2020 (diff | hist) . . (+414) . . 20.109(F20):M1D5 (→Part 4: Perform CometChip experiment)
- 00:41, 29 July 2020 (diff | hist) . . (+2) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 00:10, 29 July 2020 (diff | hist) . . (0) . . 20.109(F20):M1D2 (→Part 2: Define treatment conditions that will be used for γH2AX experiment)
- 00:08, 29 July 2020 (diff | hist) . . (+470) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 23:56, 28 July 2020 (diff | hist) . . (+3) . . 20.109(F20):M1D2 (→Part 2: Define treatment conditions that will be used for γH2AX experiment)
- 23:53, 28 July 2020 (diff | hist) . . (+547) . . 20.109(F20):M1D2 (→Part 2: Define treatment conditions that will be used for γH2AX experiment)
- 23:39, 28 July 2020 (diff | hist) . . (+4) . . 20.109(F20):Module 1 (→Overview) (current)
- 23:36, 28 July 2020 (diff | hist) . . (+12) . . 20.109(F20):M1D2 (→Introduction)
- 20:35, 28 July 2020 (diff | hist) . . (+37) . . 20.109(F20):M1D1 (→Reagents list)
- 20:22, 28 July 2020 (diff | hist) . . (+197) . . 20.109(F20):M1D2 (→Part 3: Treat cells for γH2AX assay)
- 20:22, 28 July 2020 (diff | hist) . . (+3) . . 20.109(F20):M1D2 (→Part 1: Seed cells for γH2AX assay)
- 20:21, 28 July 2020 (diff | hist) . . (+85) . . 20.109(F20):M1D2 (→Protocols)
- 19:57, 28 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M1D5 (→Electrophorese CometChip)
- 19:51, 28 July 2020 (diff | hist) . . (+9) . . 20.109(F20):M1D4 (→Part 2: Complete staining for gamma-H2AX assay)
- 18:47, 28 July 2020 (diff | hist) . . (-47) . . 20.109(F20):M2D3 (→Reagents list)
- 18:46, 28 July 2020 (diff | hist) . . (-4) . . 20.109(F20):M2D3 (→Part 1: Concentrate PF3D7_1351100 protein solution)
- 18:45, 28 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M2D3 (→Reagents list)
- 18:42, 28 July 2020 (diff | hist) . . (-18) . . 20.109(F20):M2D3 (→Part 3: Measure protein concentration)
- 18:38, 28 July 2020 (diff | hist) . . (+14) . . 20.109(F20):M2D3 (→Part 3: Measure protein concentration)
- 18:36, 28 July 2020 (diff | hist) . . (+27) . . 20.109(F20):M2D3 (→Part 2: Visualize PF3D7_1351100 protein using polyacrylamide gel electrophoresis (PAGE))
- 18:35, 28 July 2020 (diff | hist) . . (-1) . . 20.109(F20):M2D3 (→Part 2: Visualize PF3D7_1351100 protein using polyacrylamide gel electrophoresis (PAGE))
- 18:32, 28 July 2020 (diff | hist) . . (+88) . . 20.109(F20):M2D3 (→Part 3: Measure protein concentration)
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