Difference between revisions of "20.109(S24):Module 2"
Noreen Lyell (Talk | contribs) (Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> {{Template:20.109(S24)}}") |
Becky Meyer (Talk | contribs) |
||
| Line 1: | Line 1: | ||
<div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> | <div style="padding: 10px; width: 820px; border: 5px solid #434a43;"> | ||
{{Template:20.109(S24)}} | {{Template:20.109(S24)}} | ||
| + | |||
| + | |||
| + | |||
| + | ==Module 2: protein engineering== | ||
| + | Heavy metal environmental contamination is an increasing concern. Heavy metals are released into the environment during activities like mining and fertilizer release in farming. Additionally, heavy metals are released into the environment from the disposal of electronic waste. As such, the ability to remove the metal contamination from soil and water is a topic of great interest. | ||
| + | |||
| + | One way to approach this problem is by harnessing known biological mechanisms and repurpose them to clean environmental pollution. This is known as bioremediation. In this module, we will use protein engineering to attempt to create a model system for bioremediation by taking advantage of a genetically tractable model organism Saccharomyces cerevisiae (baker's yeast). We will be utilizing a version of this organism that has been genetically modified to produce hydrogen sulfate, which will precipitate cadmium in the media. | ||
| + | |||
| + | Our aim in Mod 2 is to use this strain of hydrogen sulfide producing yeast and engineer a cell surface display system to display a peptide of your design in order to capture precipitating cadmium. We will then assess the quantity of cadmium captured as well as it's quality for recycling back into the manufacturing process. | ||
| + | |||
| + | |||
| + | <font color= #015526 >'''Research goal: Genetically engineer a cell surface display peptide to capture cadmium in a model for bioremediation '''</font color> | ||
| + | |||
| + | |||
| + | |||
| + | [[Image:Sp24 Mod2 overview.png|center|750px|thumb|Image generated using BioRender.]] | ||
| + | |||
| + | |||
| + | <br style="clear:both;"/> | ||
| + | |||
| + | ==Lab links: day by day== | ||
| + | M2D1: [[20.109(S24):M2D1 |Determine mutagenesis strategy]] <br> | ||
| + | M2D2: [[20.109(S24):M2D2 |Perform site-directed mutagenesis]] <br> | ||
| + | M2D3: [[20.109(S24):M2D3 |Sequence clones and transform into yeast cells]] <br> | ||
| + | M2D4: [[20.109(S24):M2D4 |Determine transporter mutation and prepare metal uptake experiment]] <br> | ||
| + | M2D5: [[20.109(S24):M2D5 | Analyze ICP-OES data]] <br> | ||
| + | M2D6: [[20.109(S24):M2D6 | IF staining and prepare for cytotoxicity experiment]] <br> | ||
| + | M2D7: [[20.109(S24):M2D7 |Confirm transporter expression and cell survival of yeast exposed to metal]] <br> | ||
| + | M2D8: [[20.109(S24):M2D8 |Complete data analysis and organize Research article figures]] <br> | ||
| + | |||
| + | ==Major assignments== | ||
| + | [[20.109(S24):Journal article presentation| Journal article presentation]] <br> | ||
| + | [[20.109(S24):Research article|Research article]] <br> | ||
| + | |||
| + | ==References== | ||
| + | |||
| + | |||
| + | ==Notes for Instructors== | ||
| + | [[20.109(S24): Prep notes for M2| Prep notes for M2]] | ||
Revision as of 19:52, 28 February 2024
Contents
Module 2: protein engineering
Heavy metal environmental contamination is an increasing concern. Heavy metals are released into the environment during activities like mining and fertilizer release in farming. Additionally, heavy metals are released into the environment from the disposal of electronic waste. As such, the ability to remove the metal contamination from soil and water is a topic of great interest.
One way to approach this problem is by harnessing known biological mechanisms and repurpose them to clean environmental pollution. This is known as bioremediation. In this module, we will use protein engineering to attempt to create a model system for bioremediation by taking advantage of a genetically tractable model organism Saccharomyces cerevisiae (baker's yeast). We will be utilizing a version of this organism that has been genetically modified to produce hydrogen sulfate, which will precipitate cadmium in the media.
Our aim in Mod 2 is to use this strain of hydrogen sulfide producing yeast and engineer a cell surface display system to display a peptide of your design in order to capture precipitating cadmium. We will then assess the quantity of cadmium captured as well as it's quality for recycling back into the manufacturing process.
Research goal: Genetically engineer a cell surface display peptide to capture cadmium in a model for bioremediation
Lab links: day by day
M2D1: Determine mutagenesis strategy
M2D2: Perform site-directed mutagenesis
M2D3: Sequence clones and transform into yeast cells
M2D4: Determine transporter mutation and prepare metal uptake experiment
M2D5: Analyze ICP-OES data
M2D6: IF staining and prepare for cytotoxicity experiment
M2D7: Confirm transporter expression and cell survival of yeast exposed to metal
M2D8: Complete data analysis and organize Research article figures
Major assignments
Journal article presentation
Research article
