Difference between revisions of "20.109(S20):Class data"

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===M1D7: Evaluate class hits===
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*Red Team: [[ ]]
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*Orange Team: [[ ]]
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*Yellow Team: [[ ]]
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*Green Team: [[ ]]
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*Light Blue Team: [[ ]]
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*Pink Team: [[ ]]
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*Purple Team: [[ File:Top5Hits.xlsx]]
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*White Team: [[ ]]
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*Silver Team: [[ ]]
  
 
==Module 2: Measuring gene expression==
 
==Module 2: Measuring gene expression==
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[[Media:Sp20 M2 qPCR pilot.xlsx | qPCR data]]
  
 
==Module 3: Engineering antibodies==
 
==Module 3: Engineering antibodies==
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===M3D3: Sequencing results===
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[[Media:Benchling_Aligning_to_template.docx |Benchling Sequence Alignment]]<br>
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[[Media:Sp20M3_Clone2&5seq_abi.zip |Sequencing Data for Clone 2 and Clone 5]]
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===M3D5: Flow Cytometry Data===
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[[Media:Flow_Cytometry_Data_for_M3D5.zip|Flow Cytometry Data]]

Latest revision as of 14:34, 21 April 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 1: Screening ligand binders

M1D3: Evaluate protein purity and concentration

TR Section

BCA Assay results (A562 measurements): linked here

SDS-PAGE results:

LiCOR Alexa 647 results:

WF Section

BCA Assay results (A562 measurements): linked here

SDS-PAGE results:

LiCOR Alexa 647 results:

M1D4: Perform small molecule microarray (SMM) with TDP43 protein

SMM slide assignments:

TR Team Slide barcodes WF Team Slide barcodes
Red 0011-03, 0011-04 Red 0011-11, 0013-11
Orange 0012-09, 0012-10 Orange 0013-2, 0014-9
Yellow 0012-05, 0012-06 Yellow 0013-1, 0014-1
Green 0012-03, 0012-04 Green 0011-7, 0013-10
Blue 0012-01, 0012-02 Blue 0013-12, 0014-12
Pink 0012-03, 0012-04 Pink 0014-11, 0014-8
Purple 0012-11, 0012-12 Purple 0013-9, 0014-10
White 0011-05, 0011-06 White 0013-7, 0014-7
Grey 0012-07, 0012-08 Silver 0011-8, 0011-12

M1D7: Evaluate class hits

  • Red Team: [[ ]]
  • Orange Team: [[ ]]
  • Yellow Team: [[ ]]
  • Green Team: [[ ]]
  • Light Blue Team: [[ ]]
  • Pink Team: [[ ]]
  • Purple Team: File:Top5Hits.xlsx
  • White Team: [[ ]]
  • Silver Team: [[ ]]

Module 2: Measuring gene expression

qPCR data

Module 3: Engineering antibodies

M3D3: Sequencing results

Benchling Sequence Alignment
Sequencing Data for Clone 2 and Clone 5

M3D5: Flow Cytometry Data

Flow Cytometry Data