Difference between revisions of "20.109(S20):Class data"

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==Module 1: Screening ligand binders==
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===M1D3: Evaluate protein purity and concentration===
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 +
====TR Section====
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BCA Assay results (A<sub>562</sub> measurements):  [[Media:Sp20_TR_BCA.xlsx| linked here]]
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SDS-PAGE results:
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*Red Team: [[Media:Sp20 TR Red SDSPAGE.HEIC |image here]]
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*Orange Team: [[Media:Sp20 TR Orange SDSPAGE.HEIC |image here]]
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*Yellow Team: [[Media:Sp20 TR Yellow SDSPAGE.HEIC |image here]]
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*Green Team: [[Media:Sp20 TR Green SDSPAGE.HEIC |image here]]
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*Blue Team: [[Media:Sp20 TR Blue SDSPAGE.HEIC |image here]]
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*Pink Team: [[Media:Sp20 TR Pink SDSPAGE.HEIC |image here]]
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*Purple Team: [[Media:Sp20 TR Purple SDSPAGE.HEIC |image here]]
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*White Team: [[Media:Sp20 TR White SDSPAGE.HEIC |image here]]
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*Grey Team: [[Media:Sp20 TR Grey SDSPAGE.HEIC |image here]]
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LiCOR Alexa 647 results:
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*Red Team: [[Media:Sp20 TR Red.tif |image here]]
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*Orange Team: [[Media:TR Orange Licor.tif |image here]]
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*Yellow Team: [[Media:TR Yellow Licor.tif |image here]]
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*Green Team: [[Media:TR Green Licor.tif |image here]]
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*Blue Team: [[Media:TR Red Licor.tif |image here]]
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*Pink Team: [[Media:TR Pink Licor.tif |image here]]
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*Purple Team: [[Media:TR Purple Licor.tif |image here]]
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*White Team: [[Media:TR White Licor.tif |image here]]
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*Grey Team: [[Media:TR Grey Licor.tif |image here]]
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====WF Section====
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BCA Assay results (A<sub>562</sub> measurements):  [[Media:Sp20 WF BCA.xlsx| linked here]]
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SDS-PAGE results:
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*Red Team: [[Media:WF Red SDS.tif |image here]]
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*Orange Team: [[Media:WF Orange SDS.tif |image here]]
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*Yellow Team: [[Media:WF Yellow SDS.tif |image here]]
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*Green Team: [[Media:WF Green SDS.tif |image here]]
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*Light Blue Team: [[Media:WF Blue SDS.tif |image here]]
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*Pink Team: [[Media:WF Pink SDS.tif |image here]]
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*Purple Team: [[Media:WF Purple SDS.tif |image here]]
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*White Team: [[Media:WF White SDS.tif |image here]]
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*Silver Team: [[Media:WF Silver SDS.tif |image here]]
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LiCOR Alexa 647 results:
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*Red Team: [[Media:Sp20 WF Red.tif |image here]]
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*Orange Team: [[Media:Licor WF Orange.tif |image here]]
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*Yellow Team: [[Media:Licor WF Yellow.tif |image here]]
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*Green Team: [[Media:Licor WF Green.tif |image here]]
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*Blue Team: [[Media:Licor WF Blue.tif |image here]]
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*Pink Team: [[Media:Licor WF Pink.tif |image here]]
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*Purple Team: [[Media:Licor WF Purple.tif |image here]]
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*White Team: [[Media:Licor WF White.tif |image here]]
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*Silver Team: [[Media:Licor WF Silver.tif |image here]]
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===M1D4: Perform small molecule microarray (SMM) with TDP43 protein===
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SMM slide assignments:
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{| border="1"
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|TR Team
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|Slide barcodes
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|WF Team
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|Slide barcodes
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|-
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| Red
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| 0011-03, 0011-04
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| Red
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| 0011-11, 0013-11
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|-
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| Orange
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| 0012-09, 0012-10
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| Orange
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| 0013-2, 0014-9
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|-
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| Yellow
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| 0012-05, 0012-06
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| Yellow
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| 0013-1, 0014-1
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|-
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| Green
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| 0012-03, 0012-04
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| Green
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| 0011-7, 0013-10
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|-
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| Blue
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| 0012-01, 0012-02
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| Blue
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| 0013-12, 0014-12
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|-
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| Pink
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| 0012-03, 0012-04
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| Pink
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| 0014-11, 0014-8
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|-
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| Purple
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| 0012-11, 0012-12
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| Purple
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| 0013-9, 0014-10
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|-
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| White
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| 0011-05, 0011-06
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| White
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| 0013-7, 0014-7
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|-
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| Grey
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| 0012-07, 0012-08
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| Silver
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| 0011-8, 0011-12
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|}
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===M1D7: Evaluate class hits===
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*Red Team: [[ ]]
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*Orange Team: [[ ]]
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*Yellow Team: [[ ]]
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*Green Team: [[ ]]
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*Light Blue Team: [[ ]]
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*Pink Team: [[ ]]
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*Purple Team: [[ File:Top5Hits.xlsx]]
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*White Team: [[ ]]
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*Silver Team: [[ ]]
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==Module 2: Measuring gene expression==
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[[Media:Sp20 M2 qPCR pilot.xlsx | qPCR data]]
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==Module 3: Engineering antibodies==
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===M3D3: Sequencing results===
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[[Media:Benchling_Aligning_to_template.docx |Benchling Sequence Alignment]]<br>
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[[Media:Sp20M3_Clone2&5seq_abi.zip |Sequencing Data for Clone 2 and Clone 5]]
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===M3D5: Flow Cytometry Data===
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[[Media:Flow_Cytometry_Data_for_M3D5.zip|Flow Cytometry Data]]

Latest revision as of 14:34, 21 April 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 1: Screening ligand binders

M1D3: Evaluate protein purity and concentration

TR Section

BCA Assay results (A562 measurements): linked here

SDS-PAGE results:

LiCOR Alexa 647 results:

WF Section

BCA Assay results (A562 measurements): linked here

SDS-PAGE results:

LiCOR Alexa 647 results:

M1D4: Perform small molecule microarray (SMM) with TDP43 protein

SMM slide assignments:

TR Team Slide barcodes WF Team Slide barcodes
Red 0011-03, 0011-04 Red 0011-11, 0013-11
Orange 0012-09, 0012-10 Orange 0013-2, 0014-9
Yellow 0012-05, 0012-06 Yellow 0013-1, 0014-1
Green 0012-03, 0012-04 Green 0011-7, 0013-10
Blue 0012-01, 0012-02 Blue 0013-12, 0014-12
Pink 0012-03, 0012-04 Pink 0014-11, 0014-8
Purple 0012-11, 0012-12 Purple 0013-9, 0014-10
White 0011-05, 0011-06 White 0013-7, 0014-7
Grey 0012-07, 0012-08 Silver 0011-8, 0011-12

M1D7: Evaluate class hits

  • Red Team: [[ ]]
  • Orange Team: [[ ]]
  • Yellow Team: [[ ]]
  • Green Team: [[ ]]
  • Light Blue Team: [[ ]]
  • Pink Team: [[ ]]
  • Purple Team: File:Top5Hits.xlsx
  • White Team: [[ ]]
  • Silver Team: [[ ]]

Module 2: Measuring gene expression

qPCR data

Module 3: Engineering antibodies

M3D3: Sequencing results

Benchling Sequence Alignment
Sequencing Data for Clone 2 and Clone 5

M3D5: Flow Cytometry Data

Flow Cytometry Data
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