Difference between revisions of "20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)"

From Course Wiki
Jump to: navigation, search
(Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #A4A4A4;"> {{Template:20.109(F19)}} ==Introduction== <font color = red> CETSA </font color> ==Protocols== ===Pa...")
 
(Protocols)
Line 11: Line 11:
 
===Part 1: Incubate cells with ligand===
 
===Part 1: Incubate cells with ligand===
  
===Part 2: Apply heat treatment===
+
===Part 2: Apply heat treatment and snap freeze cells===
 
+
===Part 3: Snap freeze cells===
+
  
 
==Reagents list==
 
==Reagents list==

Revision as of 20:48, 1 September 2019

20.109(F19): Laboratory Fundamentals of Biological Engineering

Fa19 20109 Banner image.png

Fall 2019 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Testing chemical probes              


Introduction

CETSA

Protocols

Part 1: Incubate cells with ligand

Part 2: Apply heat treatment and snap freeze cells

Reagents list

Navigation links

Next day: Begin Western blot analysis

Previous day: Prepare for cellular thermal shift assay