Difference between revisions of "20.109(F17): Notes for M1"
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*1ml of trypsin | *1ml of trypsin | ||
*set aside T75 flask | *set aside T75 flask | ||
| + | *printout of today's protocol | ||
Per group | Per group | ||
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===M1D2=== | ===M1D2=== | ||
| + | Per group, in Tissue Culture, sterile | ||
| + | *10 mL MEF media | ||
| + | *1ml trypsin | ||
| + | *5ml of PBS | ||
| + | *printout of today's protocol | ||
| + | |||
Per group, in main lab | Per group, in main lab | ||
*1x PBS in glass bottle | *1x PBS in glass bottle | ||
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*40ml Neutralization buffer | *40ml Neutralization buffer | ||
*print a large scheme of the plate so they can keep track of how to load chips | *print a large scheme of the plate so they can keep track of how to load chips | ||
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Latest revision as of 18:12, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep)
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
- printout of today's protocol
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
- Secure line II pen
- scraped lid for comet chip stamp
M1D2
Per group, in Tissue Culture, sterile
- 10 mL MEF media
- 1ml trypsin
- 5ml of PBS
- printout of today's protocol
Per group, in main lab
- 1x PBS in glass bottle
- 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- 40ml alkaline lysis solution
- 40ml Neutralization buffer
- print a large scheme of the plate so they can keep track of how to load chips
