20.109(F17): Notes for M1

M1D1

Per group, aliquot in Tissue Culture, sterile

• 14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep)
• 3ml of PBS
• 1ml of trypsin
• set aside T75 flask
• printout of today's protocol

Per group

• 50 mL 1x PBS in bottle
• cylinder near weight station for PBS
• 100ml glass bottles near weigh station
• pre-cut gel bond at front bench
• Secure line II pen
• scraped lid for comet chip stamp

M1D2

Per group, in Tissue Culture, sterile

• 10 mL MEF media
• 1ml trypsin
• 5ml of PBS
• printout of today's protocol

Per group, in main lab

• 1x PBS in glass bottle
• 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
• 4 binder clips
• 1 glass slide
• 1 bottomless 96-well plate
• 1 razor blade
• Pasteur pipets
• sharp jar
• pipet aid
• 40ml alkaline lysis solution
• 40ml Neutralization buffer
• print a large scheme of the plate so they can keep track of how to load chips