Difference between revisions of "20.109(F17): Notes for M1"
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(Created page with "===M1D1=== Per group, aliquot *50 mL 1x PBS *in TC room, 15 mL of “TK6 media” ===M1D2=== Per group, aliquot *in main lab **10 mL 1x PBS **2 eppendorf tubes of 0.3% LMP a...") |
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+ | {{Template:20.109(F17)}} | ||
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===M1D1=== | ===M1D1=== | ||
− | Per group, aliquot | + | Per group, aliquot in Tissue Culture, sterile |
− | *50 mL 1x PBS | + | *14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep) |
− | * | + | *3ml of PBS |
+ | *1ml of trypsin | ||
+ | *set aside T75 flask | ||
+ | *printout of today's protocol | ||
+ | |||
+ | Per group | ||
+ | *50 mL 1x PBS in bottle | ||
+ | *cylinder near weight station for PBS | ||
+ | *100ml glass bottles near weigh station | ||
+ | *pre-cut gel bond at front bench | ||
+ | *Secure line II pen | ||
+ | *scraped lid for comet chip stamp | ||
+ | |||
===M1D2=== | ===M1D2=== | ||
− | Per group, | + | Per group, in Tissue Culture, sterile |
− | + | *10 mL MEF media | |
− | + | *1ml trypsin | |
− | ** | + | *5ml of PBS |
− | * | + | *printout of today's protocol |
− | + | ||
− | * | + | Per group, in main lab |
− | + | *1x PBS in glass bottle | |
− | + | *2 eppendorf tubes of LMP agarose (in eppendorf water bath) | |
− | + | ||
− | + | ||
*4 binder clips | *4 binder clips | ||
*1 glass slide | *1 glass slide | ||
Line 22: | Line 35: | ||
*sharp jar | *sharp jar | ||
*pipet aid | *pipet aid | ||
− | * | + | *40ml alkaline lysis solution |
− | * | + | *40ml Neutralization buffer |
− | + | *print a large scheme of the plate so they can keep track of how to load chips | |
− | *large scheme of the plate | + |
Latest revision as of 18:12, 11 September 2017
M1D1
Per group, aliquot in Tissue Culture, sterile
- 14ml of MEF media (DMEM, 10% FBS, 1X Pen/Strep)
- 3ml of PBS
- 1ml of trypsin
- set aside T75 flask
- printout of today's protocol
Per group
- 50 mL 1x PBS in bottle
- cylinder near weight station for PBS
- 100ml glass bottles near weigh station
- pre-cut gel bond at front bench
- Secure line II pen
- scraped lid for comet chip stamp
M1D2
Per group, in Tissue Culture, sterile
- 10 mL MEF media
- 1ml trypsin
- 5ml of PBS
- printout of today's protocol
Per group, in main lab
- 1x PBS in glass bottle
- 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- 40ml alkaline lysis solution
- 40ml Neutralization buffer
- print a large scheme of the plate so they can keep track of how to load chips