Difference between revisions of "20.109(F17): Notes for M1"

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(→‎M1D1)
(→‎M1D2)
Line 18: Line 18:
 
Per group, aliquot
 
Per group, aliquot
 
*in main lab
 
*in main lab
−
**10 mL 1x PBS  
+
**1x PBS glass bottle
−
**2 eppendorf tubes of 0.3% LMP agarose (in eppendorf water bath)
+
**2 eppendorf tubes of LMP agarose (in eppendorf water bath)
−
**large scheme of the plate so they can keep track of how to load chips
+
**print a large scheme of the plate so they can keep track of how to load chips
 +
 
 
*and separately, in the TC hood,  
 
*and separately, in the TC hood,  
−
**10 mL 1x PBS
+
**5 mL 1x PBS
−
**25 mL TK6 media
+
**10 mL MEF media  
−
**2 eppendorf tubes of 0.3% LMP agarose (in water bath '''at 43 °C''')
+
 
−
**2x 15 mL of TK6 cells at 500,000 cells/mL
+
−
Set up hoods in TC room with, per group:
+
 
*4 binder clips
 
*4 binder clips
 
*1 glass slide
 
*1 glass slide
Line 34: Line 33:
 
*sharp jar
 
*sharp jar
 
*pipet aid
 
*pipet aid
−
*pen
+
 
 
*team sticker to identify glass slides
 
*team sticker to identify glass slides
 
*printout of today's protocol
 
*printout of today's protocol
 
*large scheme of the plate to hang in TC so they can keep track of how to load chips
 
*large scheme of the plate to hang in TC so they can keep track of how to load chips

Revision as of 17:44, 11 September 2017

20.109(F17): Laboratory Fundamentals of Biological Engineering

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       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

M1D1

Per group, aliquot in Tissue Culture, sterile

  • 14ml of DMEM media
  • 3ml of PBS
  • 1ml of trypsin
  • set aside T75 flask

Per group

  • 50 mL 1x PBS in bottle
  • cylinder near weight station for PBS
  • 100ml glass bottles near weigh station
  • pre-cut gel bond at front bench

M1D2

Per group, aliquot

  • in main lab
    • 1x PBS glass bottle
    • 2 eppendorf tubes of LMP agarose (in eppendorf water bath)
    • print a large scheme of the plate so they can keep track of how to load chips
  • and separately, in the TC hood,
    • 5 mL 1x PBS
    • 10 mL MEF media
  • 4 binder clips
  • 1 glass slide
  • 1 bottomless 96-well plate
  • 1 razor blade
  • Pasteur pipets
  • sharp jar
  • pipet aid
  • team sticker to identify glass slides
  • printout of today's protocol
  • large scheme of the plate to hang in TC so they can keep track of how to load chips
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