Difference between revisions of "20.109(F17): Notes for M1"
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Revision as of 17:37, 11 September 2017
M1D1
Per group, aliquot
- 50 mL 1x PBS
- in TC room, 15 mL of “TK6 media”
M1D2
Per group, aliquot
- in main lab
- 10 mL 1x PBS
- 2 eppendorf tubes of 0.3% LMP agarose (in eppendorf water bath)
- large scheme of the plate so they can keep track of how to load chips
- and separately, in the TC hood,
- 10 mL 1x PBS
- 25 mL TK6 media
- 2 eppendorf tubes of 0.3% LMP agarose (in water bath at 43 °C)
- 2x 15 mL of TK6 cells at 500,000 cells/mL
Set up hoods in TC room with, per group:
- 4 binder clips
- 1 glass slide
- 1 bottomless 96-well plate
- 1 razor blade
- Pasteur pipets
- sharp jar
- pipet aid
- pen
- team sticker to identify glass slides
- printout of today's protocol
- large scheme of the plate to hang in TC so they can keep track of how to load chips
