Difference between revisions of "20.109(S17):Confirm cell lines and practice tissue culture techniques (Day1)"
From Course Wiki
Noreen Lyell (Talk | contribs) (→Navigation links) |
Noreen Lyell (Talk | contribs) (→Protocols) |
||
| Line 5: | Line 5: | ||
==Protocols== | ==Protocols== | ||
| + | |||
| + | ===Part 1: Practice tissue culture techniques=== | ||
| + | |||
| + | ====Part 1a: Demonstration of best practices==== | ||
| + | |||
| + | ====Part 1b: Seed cells for survival assay==== | ||
| + | |||
| + | ===Part 2: Prepare cell lysates for Western blot=== | ||
==Reagents== | ==Reagents== | ||
Revision as of 00:45, 1 February 2017
Contents
Introduction
Protocols
Part 1: Practice tissue culture techniques
Part 1a: Demonstration of best practices
Part 1b: Seed cells for survival assay
Part 2: Prepare cell lysates for Western blot
Reagents
Next day: Complete Western blot and induce DNA damage for survival and quantitative PCR assays
Previous day: Last day of M1