Difference between revisions of "20.109(F23):Fall 2023 schedule"
Noreen Lyell (Talk | contribs) |
|||
(46 intermediate revisions by 3 users not shown) | |||
Line 41: | Line 41: | ||
| T/W Sep 19/20 | | T/W Sep 19/20 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> [[Media:BE109 Fall23 Mod1 Day3 HANDOUT.pdf| Lecture slides]] | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> [[Media:BE109 Fall23 Mod1 Day3 HANDOUT.pdf| Lecture slides]] | ||
− | | [[20.109(F23):M1D3 | Use immunoflourescence staining to assess γH2AX experiment]]<br> | + | | [[20.109(F23):M1D3 | Use immunoflourescence staining to assess γH2AX experiment]]<br> [[Media:Fa23 M1D3 bcm.pdf | T/R prelab]]<br>[[Media:Fa23_M1D3_jz_fixed.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M1D3|Homework due]] | | [[20.109(F23):Homework#Due_M1D3|Homework due]] | ||
|-- | |-- | ||
Line 52: | Line 52: | ||
| M1D4 | | M1D4 | ||
| T/W Sep 26/27 | | T/W Sep 26/27 | ||
− | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> | + | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> [[Media:BE109 Fall23 Mod1 Day4 HANDOUT.pdf| Lecture slides]] |
− | | [[20.109(F23):M1D4 | Complete data analysis for γH2AX experiment]]<br> | + | | [[20.109(F23):M1D4 | Complete data analysis for γH2AX experiment]]<br> [[Media:Fa23 M1D4 bcm.pdf | T/R prelab]] <br>[[Media:Fa23_M1D4_jz.pdf | W/F prelab]] |
| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M1D4|Homework due]] | | <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M1D4|Homework due]] | ||
|-- | |-- | ||
| M1D5 | | M1D5 | ||
| R/F Sep 28/29 | | R/F Sep 28/29 | ||
− | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> | + | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> [[Media:BE109 Fall23 Mod1 Day5 HANDOUT.pdf| Lecture slides]] |
− | | [[20.109(F23):M1D5 | Treat cells for CometChip assay]]<br> | + | | [[20.109(F23):M1D5 | Treat cells for CometChip assay]]<br> [[Media:Fa23 M1D5 bcm.pdf| T/R prelab]] <br>[[Media:Fa23_M1D5_jz.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M1D5|Homework due]] <br> [[20.109(F23):Research talk| <font color = #227d49 >'''Research talk due'''</font color>]] Sat, Sep 30 at 10 pm | | [[20.109(F23):Homework#Due_M1D5|Homework due]] <br> [[20.109(F23):Research talk| <font color = #227d49 >'''Research talk due'''</font color>]] Sat, Sep 30 at 10 pm | ||
|-- | |-- | ||
| M1D6 | | M1D6 | ||
| T/W Oct 3/4 | | T/W Oct 3/4 | ||
− | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> | + | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> [[Media:BE109 Fall23 Mod1 Day6 HANDOUT.pdf| Lecture slides]] |
− | | [[20.109(F23):M1D6 | Image and analyze data for CometChip assay]]<br> | + | | [[20.109(F23):M1D6 | Image and analyze data for CometChip assay]]<br> [[Media:Fa23 M1D6 bcm.pdf | T/R prelab]] <br>[[Media:Fa23_M1D6_jz.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M1D6|Homework due]] | | [[20.109(F23):Homework#Due_M1D6|Homework due]] | ||
|-- | |-- | ||
Line 71: | Line 71: | ||
| R/F Oct 5/6 | | R/F Oct 5/6 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BPE] <br> | ||
− | | [[20.109(F23):M1D7 | Examine experimental data using statistical methods]]<br> | + | | [[20.109(F23):M1D7 | Examine experimental data using statistical methods]]<br> [[Media:Fa23 M1D8 nll.pdf| T/R prelab]]<br> [[Media:Fa23 M1D8 nll.pdf| W/F prelab]] <br> [[Media:Fa23 DS discussion.pdf| Data summary discussion]] |
| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M1D7|Homework due]] | | <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M1D7|Homework due]] | ||
|-- | |-- | ||
Line 82: | Line 82: | ||
| M2D1 | | M2D1 | ||
| R/F Oct 12/13 | | R/F Oct 12/13 | ||
− | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | + | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> [[Media:L01 Intro Drug Discovery 20109 2023-10-12 presented).pdf| Lecture slides]] |
− | | [[20.109(F23):M2D1 |Complete in-silico cloning of protein expression plasmid ]]<br> | + | | [[20.109(F23):M2D1 |Complete in-silico cloning of protein expression plasmid ]]<br> [[Media:Fa23 M2D1 bcm.pdf | T/R prelab]]<br>[[Media:Fa23_M2D1_jz.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M2D1|Homework due]] | | [[20.109(F23):Homework#Due_M2D1|Homework due]] | ||
|-- | |-- | ||
Line 89: | Line 89: | ||
| T/W Oct 17/18 | | T/W Oct 17/18 | ||
| BE Comm Lab <br> | | BE Comm Lab <br> | ||
− | | [[20.109(F23):M2D2 |Perform protein purification protocol]] <br> | + | | [[20.109(F23):M2D2 |Perform protein purification protocol]] <br> [[Media:Fa23 M2D2 saw.pdf | T/R prelab]]<br>[[Media:Fa23_M2D2_jz.pdf | W/F prelab]]<br> [[Media:Fa23 JADiscussion nll.pdf| Journal article presentation discussion]] |
| [[20.109(F23):Homework#Due_M2D2|Homework due]] | | [[20.109(F23):Homework#Due_M2D2|Homework due]] | ||
|-- | |-- | ||
| M2D3 | | M2D3 | ||
| R/F Oct 19/20 | | R/F Oct 19/20 | ||
− | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | + | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> [[Media:L02 Therapeutic Target Selection 20.109 2023-10-19 (Niles).pdf| Lecture slides]] |
− | | [[20.109(F23):M2D3 |Assess purity and concentration of purified protein]] <br> | + | | [[20.109(F23):M2D3 |Assess purity and concentration of purified protein]] <br> [[Media:Fa23 M2D3 bcm.pdf | T/R prelab]]<br>[[Media:Fa23_M2D3_jz.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M2D3|Homework due]] <br> [[20.109(F23):Data Summary| <font color = #227d49 >'''Data Summary revision due'''</font color>]] Sat, Oct 21 at 10 pm <br> | | [[20.109(F23):Homework#Due_M2D3|Homework due]] <br> [[20.109(F23):Data Summary| <font color = #227d49 >'''Data Summary revision due'''</font color>]] Sat, Oct 21 at 10 pm <br> | ||
|-- | |-- | ||
Line 112: | Line 112: | ||
| M2D4 | | M2D4 | ||
| T/W Oct 30/Nov 1 | | T/W Oct 30/Nov 1 | ||
− | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | + | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> [[Media:Lecture 03 Choosing an intervention modality 20.109 2023-10-31 (Niles).pdf| Lecture slides]] |
− | | [[20.109(F23):M2D4 | | + | | [[20.109(F23):M2D4 |Evaluate protein structures]] <br> [[Media:Fa23 M2D4 bcm.pdf| T/R prelab]] <br> [[Media:Fa23 M2D4 bcm.pdf| W/F prelab]] |
| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D4|Homework due]] | | <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D4|Homework due]] | ||
|-- | |-- | ||
| M2D5 | | M2D5 | ||
| R/F Nov 2/3 | | R/F Nov 2/3 | ||
− | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | + | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> [[Media:L04 Intro to Screening 1.pdf| Lecture slides]] |
− | | [[20.109(F23):M2D5 |Prepare protein for functional assay]] <br> | + | | [[20.109(F23):M2D5 |Prepare protein for functional assay]] <br> [[Media:Fa23 M2D5 bcm.pdf | T/R prelab]] <br> [[Media:Fa23 M2D5 bcm.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M2D5|Homework due]] | | [[20.109(F23):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
| M2D6 | | M2D6 | ||
| T/W Nov 7/8 | | T/W Nov 7/8 | ||
− | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | + | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> [[Media:L05 Intro to Screening II Phenotypic Screens (Niles).pdf| Lecture slides]] |
− | | [[20.109(F23):M2D6 |Complete data analysis of functional assay results]] <br> | + | | [[20.109(F23):M2D6 |Complete data analysis of functional assay results]] <br> [[Media:Fa23 M2D6 bcm.pdf | T/R prelab]] <br>[[Media:Fa23 M2D6 bcm.pdf | W/F prelab]] |
| [[20.109(F23):Homework#Due_M2D5|Homework due]] | | [[20.109(F23):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
Line 137: | Line 137: | ||
| T/W Nov 14/15 | | T/W Nov 14/15 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JCN] <br> | ||
− | | [[20.109(F23):M2D7 | | + | | [[20.109(F23):M2D7 |Organize results and incorporate class data]] <br> [[Media:Fa23 M2D7 bcm.pdf | T/R prelab]] <br> [[Media:Fa23 M2D7 bcm.pdf | W/F prelab]]<br> [[Media:Fa23 RA discussion.pdf|Research article discussion]] |
| <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D7|Homework due]] | | <font color= #d57830 >'''Laboratory quiz'''</font color> <br> [[20.109(F23):Homework#Due_M2D7|Homework due]] | ||
|-- | |-- | ||
Line 143: | Line 143: | ||
| R/F Nov 16/17 | | R/F Nov 16/17 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> | ||
− | | [[20.109(F23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> | + | | [[20.109(F23):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> [[Media:Fa23_M3D1_nll.pdf | T/R prelab]] <br> [[Media:Fa23_M3D1_nll.pdf | W/F prelab]] |
− | | [[20.109(F23):Homework#Due_M3D1|Homework due]] | + | | [[20.109(F23):Homework#Due_M3D1|Homework due]] |
|-- | |-- | ||
| | | | ||
Line 161: | Line 161: | ||
| T/W Nov 28/29 | | T/W Nov 28/29 | ||
| [http://be.mit.edu/directory/angela-belcher AB]<br> | | [http://be.mit.edu/directory/angela-belcher AB]<br> | ||
− | | [[20.109(F23):M3D2 |Pitch research proposal presentation ideas ]] <br> | + | | [[20.109(F23):M3D2 |Pitch research proposal presentation ideas ]] <br> [[Media:Fa23 M3D2 nll.pdf|T/R prelab]] <br> [[Media:Fa23_M3D2_jz.pdf|W/F prelab]] |
| | | | ||
|-- | |-- | ||
Line 167: | Line 167: | ||
| R/F Nov 30/Dec1 | | R/F Nov 30/Dec1 | ||
| BE Comm Lab | | BE Comm Lab | ||
− | | [[20.109(F23):M3D3 |Develop ideas for Research proposal presentation ]] <br> | + | | [[20.109(F23):M3D3 |Develop ideas for Research proposal presentation ]] <br> [[Media:Fa23 M3D3 nll.pdf|T/R prelab]] <br> [[Media:Fa23_M3D3_jz.pdf|W/F prelab]] |
| [[20.109(F23):Homework#Due_M3D3|Homework due]]<br> | | [[20.109(F23):Homework#Due_M3D3|Homework due]]<br> | ||
|-- | |-- |
Latest revision as of 17:39, 1 December 2023
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 16-220
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322
ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).