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• Cellular microrheology
  ... <ref>[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1302394/ Yiider Tseng, Thomas P Kole, and Denis Wirtz, "Micromechanical mapping of live cells by multiple
  5 KB (750 words) - 18:34, 18 October 2013
• 20.109(S08):DNA engineering/DNA engineering by PCR (Day 1)
  ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u ...then analyze your sequence. If your melting temperature (Tm) is not 60&deg;C try adding or deleting bases from your landing sequence and repeating the a
  27 KB (3,752 words) - 14:24, 5 June 2015
• 20.109(S08):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://web.mit.edu/engelward-lab/publica
  4 KB (637 words) - 14:24, 5 June 2015
• 20.109(F08):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://web.mit.edu/engelward-lab/publica
  3 KB (512 words) - 19:05, 28 July 2015
• 20.109(F08):DNA engineering/DNA engineering by PCR (Day 1)
  ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u ...then analyze your sequence. If your melting temperature (Tm) is not 60&deg;C try adding or deleting bases from your landing sequence and repeating the a
  23 KB (3,892 words) - 19:30, 28 July 2015
• 20.109(F08): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.org/wiki/20 ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  22 KB (3,807 words) - 19:29, 28 July 2015
• 20.109(F08): Mod 1 Day 7 Lipofection
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [http://openwet *7. Return the plate to the 37&deg;C incubator.
  10 KB (1,701 words) - 19:05, 28 July 2015
• 20.109(F09):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://web.mit.edu/engelward-lab/publica
  3 KB (506 words) - 19:38, 28 July 2015
• 20.109(F09): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.org/wiki/20 ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  22 KB (3,783 words) - 19:44, 28 July 2015
• 20.109(F09): Mod 1 Day 7 Lipofection
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [http://openwet ...different things we might test, and we will use wells labeled 'A' 'B' and 'c' to test a parameter that might affect the frequency of inter-plasmid homol
  10 KB (1,594 words) - 19:38, 28 July 2015
• 20.109(F10):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://web.mit.edu/engelward-lab/publica
  3 KB (504 words) - 21:00, 28 July 2015
• 20.109(F10): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.org/wiki/20 ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  22 KB (3,783 words) - 21:00, 28 July 2015
• 20.109(F11):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://web.mit.edu/engelward-lab/publica
  3 KB (501 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [http://openwetware.org/wiki/20 ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  22 KB (3,809 words) - 21:08, 28 July 2015
• 20.109(F11): Mod 1 Day 6 Lipofection & lab practical
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [http://openwet ...different things we might test, and we will use wells labeled 'A' 'B' and 'C' to test a parameter that might affect the frequency of inter-plasmid homol
  10 KB (1,541 words) - 21:08, 28 July 2015
• 20.109(S13):Preparing cells for analysis (Day4)
  ...background reading and critical thinking about the topic. '''Check in with Thomas and get his feedback about your ideas for a few minutes before leaving toda #Add 3 mL of pre-warmed EDTA-citrate buffer, and incubate at 37 &deg;C for 10 min.
  25 KB (4,196 words) - 13:48, 29 July 2015
• 20.109(F14): Mod 1 Day 6 Lipofection and CometChip Analysis
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F14):M ...ive cells with low temperature agarose (this means it melts around 37 &deg;C) and casting a small gel on a microscope slide. Cells were exposed to DNA d
  14 KB (2,345 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 1 DNA engineering using PCR
  ...rmation on homologous recombination, please obtain the excellent review by Thomas Helleday from the References section of the [[20.109(F14):Module 1| Module ...ll be detecting recombination between an N-terminally truncated EGFP and a C-terminally truncated version. The primers you are designing today will be u
  23 KB (3,882 words) - 14:02, 29 July 2015
• 20.109(F14): Mod 1 Day 6 Lipofection and paper discussion
  ...rize yourself with these mechanisms by (re)reading the excellent review by Thomas Helleday that you can find in the References section of the [[20.109(F12):M ...different things we might test, and we will use wells labeled 'A' 'B' and 'C' to test a parameter that might affect the frequency of inter-plasmid homol
  11 KB (1,850 words) - 14:02, 29 July 2015
• 20.109(F14):Module 1
  ...ll also test whether this N-terminally truncated EGFP can recombine with a C-terminally truncated version to regenerate full length EGFP in vivo. Finall ...er treatment'''<br>''DNA Repair'' 2007<br> Thomas Helleday, Justin Lo, Dik C. van Gent, Bevin P. Engelward<br> [http://dx.doi.org/10.1016/j.dnarep.2007.
  4 KB (575 words) - 14:02, 29 July 2015