Spring 2012:LFM

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20.345: Bioinstrumentation Project Lab

LFM Main        Literature and Interest        Proposal        Documentation        Report       

Light Field Microscopy

First Proposal

Here is the link to our proposal.

Literature Search

Light field imaging allows for the capture of the light field of a sample in a single photograph.[1] This has been recently commercialized in the Lytro Camera[2] which obtains a light field using a microlens and allows for processing of the image afterwards.
Light Field Microscope Diagram from the Stanford 2006 paper.[3]

They also documented their setup in more detail in a technical memo.[4]

Introduction to LFDisplay.[5]

Light field rendering.[6]

In 2009, the group published a better quality machine 4D Microscopy[7]

Objectives

I would be thrilled to work on this project for a number of reasons:

  • It will force me to work on image processing, I am novice status :p
  • It would be an awesome project
  • It is feasible
    • Only one additional physical component in a traditional bright field microscope
    • Lots of code
  • It is relevant
    • This microscope can be used to compose a 3-dimensional image of the sample
    • Taking 3-dimensional images quickly is a current point of interest in microscopy today
  • It is novel
    • With respect to this class, this is a novel project
    • Otherwise, this is not a new technology [6] but has been implemented into prototype microscopes in the past 7 years

Final Submission

Here is the link to our final report and documentation.

References

  1. A practical introduction to light field microscopy - Stanford 2010 (pdf)
  2. Light field camera
  3. Light field microscopy - Stanford 2006 (pdf)
  4. Optical recipes for light field microscopes - Stanford 2006 (pdf)
  5. LFDisplay: A real time system for light field microscopy
  6. 6.0 6.1 Light field rendering - Stanford 1996
  7. Recording and controlling the 4D light field in a microscope - Stanford 2009 (pdf)